Aim Extrapolation of tower CO 2 fluxes will be greatly facilitated if robust relationships between flux components and remotely sensed factors are established. Long-term measurements at five Northern Great Plains locations were used to obtain relationships between CO 2 fluxes and photosynthetically active radiation ( Q ), other on-site factors, and Normalized Difference Vegetation Index ( NDVI ) from the SPOT VEGETATION data set.Location CO 2 flux data from the following stations and years were analysed: Lethbridge, Alberta 1998 Fort Peck, MT 2000 Miles City, MT 2000 -01; Mandan, ND 1999 and Cheyenne, WY 1997-98.Results Analyses based on light-response functions allowed partitioning net CO 2 flux ( F ) into gross primary productivity ( P g ) and ecosystem respiration ( R e ). Weekly averages of daytime respiration, γ day , estimated from light responses were closely correlated with weekly averages of measured night-time respiration, γ night ( R 2 0.64 to 0.95). Daytime respiration tended to be higher than night-time respiration, and regressions of γ day on γ night for all sites were different from 1 : 1 relationships. Over 13 site-years, gross primary production varied from 459 to 2491 g CO 2 m − 2 year , and net ecosystem exchange from − 537 (source) to +610 g CO 2 m − 2 year − 1 (sink). Maximum daily ecological light-use efficiencies, ε d , max = P g /Q , were in the range 0.014 to 0.032 mol CO 2 (mol incident quanta)Main conclusions Ten-day average P g was significantly more highly correlated with NDVI than 10-day average daytime flux, P d ( R 2 = 0.46 to 0.77 for P g -NDVI and 0.05 to 0.58 for P d -NDVI relationships). Ten-day average R e was also positively correlated with NDVI , with R 2 values from 0.57 to 0.77. Patterns of the relationships of P g and R e with NDVI and other factors indicate possibilities for establishing multivariate functions allowing scaling-up local fluxes to larger areas using GIS data, temporal NDVI, and other factors.