We introduce a theory of jeu de taquin for increasing tableaux, extending fundamental work of [Sch ützenberger '77] for standard Young tableaux. We apply this to give a new combinatorial rule for the K-theory Schubert calculus of Grassmannians via K-theoretic jeu de taquin, providing an alternative to the rules of [Buch '02] and others. This rule naturally generalizes to give a conjectural root-system uniform rule for any minuscule flag variety G/P, extending [Thomas-Yong '06]. We also present analogues of results of Fomin, Haiman, Schensted and Sch ützenberger.