Group C rotavirus associated with fatal enteritis in a family outbreak

Abstract

A family outbreak of gastroenteritis involving three adults and three children is described in which diarrhoea and vomiting were the main clinical features. One infant died in whom no pathogens could be detected in either small or large intestinal postmortem samples. Stool samples from two symptomatic siblings contained rotaviruses as demonstrated by electron microscopy. Both of these faecal samples were negative when assayed in a group A specific rotavirus enzyme-linked immunosorbent assay (ELISA) and subsequent genomic analysis of these rotaviruses was suggestive of group C rotavirus. Serological evidence showed that these atypical rotaviruses were members of serogroup C. Other atypical rotaviruses in faecal samples from sporadic cases in symptomatic children were detected over a similar time period and location. These had electrophoretic RNA profiles similar to those in the family outbreak. Furthermore, seroepidemiological studies detected group C rotavirus antibody in blood donors resident in the location of the family outbreak.

Structure-Function Analysis of Rotavirus NSP2 Octamer by Using a Novel Complementation System

“…In contrast, group C rotaviruses are generally associated with limited outbreaks of diarrheal disease. The group C Bristol strain originated from a family outbreak of gastroenteritis that resulted in the death of a young child (5). Sequencing shows that NSP2c shares limited similarity (57%) and identity (35%) with NSP2a ( Fig.…”

Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

“…Group A rotaviruses cause severe diarrhoea in infants and young children, but they can also infect adults. Group C rotavirus infection occurs both in children and in adults, usually in sporadic cases or clustered outbreaks (Rodger et al, 1982;Caul et al, 1990;Jiang et al,1995; Kuzuya et al, 1996; Nilsson et al, 2000;Adah et al, 2002;Chen et al, 2002;Ji et al, 2002;Schnagl et al, 2004).…”

“…Group A rotaviruses cause severe diarrhoea in infants and young children, but they can also infect adults. Group C rotavirus infection occurs both in children and in adults, usually in sporadic cases or clustered outbreaks (Rodger et al, 1982;Caul et al, 1990;Jiang et al,1995; Kuzuya et al, 1996; Nilsson et al, 2000;Adah et al, 2002;Chen et al, 2002;Ji et al, 2002;Schnagl et al, 2004).In China, large waterborne epidemics caused by the human group B rotavirus strain adult diarrhoea rotavirus (ADRV) infected thousands of people aged between 10 and 40 years of age in the 1980s (Hung et al, 1983(Hung et al, , 1984Chen et al, 1985Chen et al, , 1990 et al, 1987). The same rotavirus was implicated in gastroenteritis outbreaks in Beijing city in 1994 and in Shijiazhuang city in 1997.…”

Characterization of Field Isolates of Porcine Group C Rotaviruses using Gene 5 (VP6) and Gene 8 (VP7) cDNA Probes

“…The recent association of human Gp C rotavirus with a fatal case of enteritis within a family 3 indicates the severity of the disease caused by this virus.…”

“…To overcome these disadvantages, rapid, sensitive, and type-specific assays have been developed by several investigators. [3][4][5][6]5 In 1991, the Minnesota Board of Animal Health reported 28 cases of AIV infection involving 9 serotypes in 110 flocks on 64 farms in 16 counties of the state of Minnesota. These cases provided an opportunity to compare a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with standard virus isolation technique (VIT) for samples obtained from commercial turkey flocks.…”