Grouping oral Candida species by multilocus enzyme electrophoresis.

Rosa, Edvaldo Antônio Ribeiro; Pereira, Cassiano Costa Silva; Rosa, Ribeiro; Höfling, José Francisco

doi:10.1099/00207713-50-3-1343

Cited by 17 publications

(11 citation statements)

References 28 publications

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“…However, according to Rosa et al. [34] MLEE patterns are only efficient for intra‐specific characterisations for systematic or epidemiological purposes. Vanhee et al.…”

Section: Discussionmentioning

confidence: 99%

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Santos

Melo

Ponzzes

et al. 2011

Mycoses

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Twenty-eight Candida albicans strains obtained from women with vaginal candidiasis were tested for phospholipase and proteinase production and clustered by multilocus enzyme electrophoresis (MLEE). The proteolytic and phospholipidic activity were considered moderate (0.56 ± 0.12 mm and 0.53 ± 0.09 mm, respectively) for all isolates. The isoenzymes malate dehydrogenase (MDH) and sorbitol dehydrogenase (SDH) showed strong intra-specific discriminatory power. The numerical and genetic interpretation of the bands produced by the isoenzymes tested presented similar discriminatory power. The genetic diversity of the isolates was measured by allelic and genic frequency, perceptual index of polymorphic loci (P = 87.5%), average number of alleles per locus, average number of alleles per polymorphic locus, average heterozygosity observed and average heterozygosity expected. We verified that three isoenzymatic loci (Adh, Gdh and Sdh-2) were not in Hardy-Weinberg equilibrium. A dendrogram constructed based on the genetic distance matrix of Nei showed seven clusters; 57.15% (16) of the isolates were considered highly related or indistinguishable, and 42.85% were considered moderately related or unrelated. We did not find a relationship between the clusters and the exoenzymes production.

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“…However, according to Rosa et al. [34] MLEE patterns are only efficient for intra‐specific characterisations for systematic or epidemiological purposes. Vanhee et al.…”

Section: Discussionmentioning

confidence: 99%

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Santos

Melo

Ponzzes

et al. 2011

Mycoses

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“…The study of 51 used SDS-PAGe and silver staining technique to identify yeast presence in the oral cavities. Another report described the characterization of Candida species from oral cavities using meticulous enzyme electrophoresis 52 .…”

Section: Discussionmentioning

confidence: 99%

Intraspecific Variations Among Strains of Penicillium aurantiogriseum using Ubiquinone and Protein Profiles

Helmy¹,

Farrag²,

Mohamadein³

2013

Biosci., Biotechnol. Res. Asia

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Ubiquinone and protein profiles of twenty nine Penicillium aurantiogriseum type strains were studied as chemotaxonomic markers. Both markers succeeded in classifying the investigated type strains into three (for ubiquinone) and four (for protein) main groups. The present study provides useful markers for the intraspecific discrimination of the investigated strains and concluded unique in profiling the ubiquinone and protein patterns using HPLC and SDS-PAGE, respectively, of twenty three P. aurantiogriseum type strains.

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“…Newer methods used to quickly speciate Candida take advantage of species-specific enzymatic characteristics [27,31,32]. Various molecular techniques, including real-time PCR [23,[33][34][35][36][37], karyotypes by pulsed field gel electrophoresis [25,38], fluorescent in situ hybridization [39,40], and pyrosequencing [41], have been developed to speed the identification of bloodborne yeasts.…”

Section: Introductionmentioning

confidence: 99%