Growing E. coli to high cell density—A historical perspective on method development

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

“…High production cost has hindered the use of PHAs, since their final price is considerably much higher than that of petrochemical-based synthetic plastic materials (30,40,41,158,266,267). Factors involved include the product yield, complexity of the technology and hence the capital cost of the plant, and the ease or difficulty of product separation.…”

“…Due to wide industrial application, PHB are considered as green plastics. Unfortunately, the current production costs are much higher than for petroleum-based synthetic plastics, which make PHAs substantially more expensive and hamper the widespread usage of this high-quality material (30,40,41). However, environmental-friendly features such as biodegradability and biocompatibility, which are lacking in conventional plastics, have continued as the commercial interest in PHAs (42).…”

Bench-scale Overproduction and Purification of recombinant GCSF in Escherichia coli fed-batch process

“…When E. coli is grown under oxygen-limited or anaerobic conditions, acetate is manufactured as main by-product (Babaeipour et al, 2013). Type of E. coli strain influences on acetate production in aerobic fermentation processes (Phue and Shiloach, 2005;Rao et al, 2008) and take places when growth rate on glucose is high, (Phue and Shiloach, 2005;Shiloach and Fass, 2005) oxygen concentration is low (Phue and Shiloach, 2005;Bylund et al, 1998) and glucose concentration is excess (Phue and Shiloach, 2005;Bylund et al, 1998;Thiry and Cingolani, 2002). Then heterogeneous condition due to scale up makes points with excess glucose and low oxygen concentration (Bylund et al, 1998), thereby acetate production is increased in larger bioreactor.…”

High Yield Production of Heterologous Proteins with Escherichia coli

“…Accumulation of nutrients at high concentration inhibits growth and recombinant protein expression 7 . High glucose causes Crabtree effect and leads to accumulation of acetate which is inhibitory to cell growth 40 .…”

“…Presence of yeast extract in the medium provides nutrients and precursors for synthesis of building blocks of cells and thus glucose is used mainly as an energy source. Presence of yeast extract in the medium also helps in lowering the inhibitory effect of acetic acid and also works as a better physiological buffer in comparison to the minimal medium 7,34,64 . Therefore, its use in the feeding medium along with glucose helped in avoiding the need of complex genetic manipulation to lower the acetic acid secretion 5 .…”