Growth and apoptosis during larval forelimb development and adult forelimb regeneration in the newt (Notophthalmus viridescens)

Vlaskalin, Tatjana; Wong, Christine J.; Tsilfidis, Catherine

doi:10.1007/s00427-004-0417-1

Cited by 63 publications

(65 citation statements)

References 29 publications

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“…The result suggests that the regenerating limb has a degree of resistance to stress, but is inadequate to counter the stress response at the very early phase, leading to a slight increase of apoptosis at the very early phase of newt limb regeneration. Our result was consistent with the findings of Vlaskalin et al, (Vlaskalin et al, 2004) who observed that there was a massive apoptosis in the adult newt forelimbs within the first 3 days post-amputation which did not appear to be present in the axolotl forelimbs, probably due to the fact that cells in the forelimb of the larval axolotl were not fully differentiated.…”

Section: Immune Response and Cell Deathsupporting

confidence: 93%

iTRAQ-based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt

Geng¹,

Guo²,

Zang³

et al. 2015

Int. J. Dev. Biol.

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The newt has the powerful capacity to regenerate lost limbs following amputation, and represents an excellent model organism to study regenerative processes. However, the molecular basis of the adaptive response in the regenerating limb of the Chinese fire-bellied newt Cynops orientalis immediately after amputation remains unclear. To better understand the adaptive response immediately after limb amputation at the protein level, we used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS methods to analyze changes in the proteome of the regenerating newt limb that occurred 2 h and 8 h after amputation. We identified 152 proteins with more than 1.5-fold change in expression compared to control. GO annotation analysis classified these proteins into several categories such as signaling, Ca 2+ binding and translocation, transcription and translation, immune response, cell death, cytoskeleton, metabolism, etc. Further ingenuity pathway analysis (IPA) showed that several signaling pathways were significantly changed at 2 h and 8 h after amputation, including EIF2 signaling, acute phase response signaling, tight junction signaling and calcium signaling, suggesting these pathways may be closely related to the adaptive response immediately after limb amputation. This work provides novel insights into understanding the molecular processes related to newt limb regeneration immediately after amputation, and a basis for further study of regenerative medicine. KEY WORDS: Cynops orientalis, limb regeneration, proteomic, stress response, cell deathAmong vertebrates, urodele salamanders possess remarkable capability to regenerate appendages from any level of amputation through blastema formation. Subsequently, blastema cells that morphologically resemble mesenchymal stem-like cells self-organize into the amputated limb parts , Nye et al., 2003. Adult newt has already been used as an important model for the limb regeneration studies. The process of limb regeneration can be divided into three major phases: wound healing and dedifferentiation; blastema accumulation and blastema growth; differentiation and morphogenesis (Iten and Bryant, 1973). Following amputation, the wound surface is covered rapidly by epithelial cells, which form the wound epidermis at the end of the stump. More importantly, a specialized epithelium provides signals to the underlying cells of Int. J. Dev. Biol. 59: 487-496 (2015) Abbreviations used in this paper: iTRAQ , isobaric tags for relative and absolute quantitation; IPA, ingenuity pathway analysis.

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Section: Immune Response and Cell Deathsupporting

confidence: 93%

iTRAQ-based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt

Geng¹,

Guo²,

Zang³

et al. 2015

Int. J. Dev. Biol.

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“…(19) Apoptotic cells have been observed during early phases of regeneration in several animals that can regenerate missing tissues, for examples planarians, xenopus, and newts. (20)(21)(22)(23)(24) Apoptotic cells have been shown to provide a number of signals that can regulate wound healing and regeneration. Mitogen-activated protein (MAP) kinases signaling through Jun kinase is important both for initiating apoptosis and production of wingless/ integrated (Wnt) and bone morphogenic protein (BMP) mitogens.…”

Section: Introductionmentioning

confidence: 99%

Stem Cell Therapy in Wound Healing and Tissue Regeneration

2016

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BACKGROUND: Recent advances in our basic knowledge of the tissue damage and regeneration pathology have combined with a remarkable progress in stem cell biology so the prospect of clinical tissue repair strategies is a tangible reality. We tried to describe a better view about mesenchymal stem cell (MSC) mechanisms in wound healing and tissue regeneration, sending any ideas for next advanced therapies.CONTENT: Sustaining injury, whether minor or major, is part of every organism life. Therefore, efficient response mechanisms to damage have developed. Wound healing is a perplexing multi-step processes which can be divided into three major phases: inflammation, proliferation, and scar formation/remodeling. Though the compartementalization of this process into discrete stages give the illusion of simplicity, but in reality it is much more complicated. So that efficient healing can occur, complex interactions between multiple cell types, soluble factors and extracellular matrix components are required to rebuild the tissue. Even under optimal conditions, the healing process drives to fibrosis or scar. The latest technology that makes a huge difference in the wound healing process is stem cell therapy, which offers a novel approach to many diseases.SUMMARY: Wound healing therapies continue to rapidly evolve, with advances in basic science and engineering research heralding the development of new therapies, as well as ways to modify existing treatments. Stem cell-based therapy is one of the most promising therapeutic concepts for wound healing. Advances in stem cell biology have enabled researchers and clinicians alike with access to cells capable of actively modulating the healing response. KEYWORDS: wound healing, tissue regeneration, stem cells therapy

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“…More recently, larval newts have been used to compare known processes of forelimb development with those of adult forelimb regeneration (Cadinouche et al, 1999;Vlaskalin et al, 2004) to better understand these processes. While urodeles have fallen out of favour for embryological studies, what has become evident is that some developmental processes are not conserved in this amphibian.…”

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confidence: 99%