Stem Cell Therapy in Wound Healing and Tissue Regeneration

Meiliana, Anna; Dewi, Nurrani Mustika; Wijaya, Andi

doi:10.18585/inabj.v8i2.191

Cited by 7 publications

(7 citation statements)

References 85 publications

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“…Studies in search for an ideal biomaterial, the synthetics or natural, as cartilage replacement is extensive as its significant needs in the field of musculoskeletal, degenerative diseases, and in otolaryngology reconstruction. Mesenchymal stem cells have ability to differentiate into tissue-specific cells and already used in most tissue engineering studies (Meiliana et al 2016). Mesenchymal stem cells (MSC) implanted in chondroitin-collagen scaffolds showed higher immunosuppression activity as compared to those implanted in collagen onlybased scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Genipin Crosslinked Blended Collagen-Chondroitin: A Promising Biomaterial Scaffold Candidate for Cartilage Reconstruction

et al. 2022

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Tissue engineering offers a solution to the shortage of materials for cartilage reconstruction surgery by providing various potential biomaterial scaffolds. Tissue engineering utilizes biological or synthetic biomaterials as a scaffold for the host cells to repopulate and regenerate the tissue. The natural biomaterials such collagen and chondroitin imitates native cartilage matrix composition. Genipin as one of natural crosslinkers was added to improve the matrix biomechanical properties. This study was done to investigate biocomposition of blended collagen type 1, collagen type 2, chondroitin sulphate (Col1-Col2-CS) and genipin for its cytotoxicity using human umbilical mesenchymal stem cells (hUCMSCs), surface morphology, and biochemical composition. Genipin-crosslinked collagen-chondroitin biocomposite showed a homogeneous shape while uncrosslinked biocomposite had rough surface and fibrillar folds size. Spectroscopy demonstrated both biocomposites had similar peak resemble to no alternation of the biocomposition by crosslinking. Both types of biocomposites were biocompatible and had no toxic effects, as compared to the cell colony only (p value = 0.26). The conclusion are blended composite of collagen chondroitin crosslinked with genipin had generated a fine microstructure scaffold with smaller pore size, had similar biomolecular component spectrum absorption, and no exhibition of residual toxicity.

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Section: Discussionmentioning

confidence: 99%

Genipin Crosslinked Blended Collagen-Chondroitin: A Promising Biomaterial Scaffold Candidate for Cartilage Reconstruction

et al. 2022

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“…It is an important normal process during tissue repair and wound healing, and this process can be increased by the paracrine effect of MSCs that secrete several angiogenic factors. 60,61 BM-MSCs, ASCs, and DPSCs increase CD31 production on endothelial cells 16,17,21,22 , but the best results were obtained in DPSCs. 17 MSCs increase pro-angiogenesis factors, such as vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor (TGF)-β, and von Willebrand factor (vWF) 16,18,20,21 , and produce proliferating cell nuclear antigen (PCNA).…”

Section: Efficacy Of Mscs In Burn Therapymentioning

confidence: 99%

“…An animal study using BM-MSCs shows that the percentage of vital tissue from the burn stasis zone is notably higher in the MSCs group (83.6±4.9%) compared to the control group (62.6±8.3%). 15 Meanwhile, rat models of burn injury treated by BM-MSCs have 33.1%±7.58% of repaired area, with less epidermal desquamation, loose collagen matrix, tissue edema, hair [55][56][57][58][59][60][61][62] Secretion of bioactive molecules from MSCs regulates immune system, angiogenesis, apoptosis, and oxidative stress. MSCs increase inflammation when the immune system is underactive and suppress inflammation when the immune system becomes overactive to avoid excessive self-attacks against healthy cells and tissues by suppressing leukocyte activity.…”

Section: Efficacy Of Mscs In Burn Therapymentioning

confidence: 99%

Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review

Dini¹,

Nugraha²,

Revina³

et al. 2022

Mol Cell Biomed Sci

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The experimental research on the use of mesenchymal stem cells (MSCs) for burn therapy has been published several times. However, current clinical procedure remains a challenging discussion. This systematic review assesses the safety and efficacy of administering mesenchymal stem cells (MSCs) to burns and determines the most effective source of MSCs for burn therapy. We reviewed several studies through PubMed, Google Scholar, Science Direct, and DOAJ online databases. PRISMA-P 2020 method was used based on inclusion and exclusion criteria that were re-selected through Joanna Briggs Institute (JBI) Critical Appraisal Tools. Results from 13 articles showed that MSCs are safe for burn therapy with minimal side effects/complications and have the potential to repair tissue and accelerate burn healing through several mechanisms. The treatment of MSCs in burns is influenced by donor characteristics and related to the severity and area of the burn. It can be concluded that the administration of MSCs is safe and effective in burn therapy. Keywords: burns, mesenchymal stem cells, therapeutic safety, therapeutic efficacy, wound healing

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“…Recently, cell therapy using stem cells has the potential to accelerate the healing of chronic wounds such as burns and diabetic wounds [1,2]. Adipose Tissue Mesenchymal Stem Cells (AT-MSCs) are stem cells derived from fatty tissue that have the ability to differentiate into other cells that are osteogenic, myogenic, neurogenic, and hematopoietic.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Microencapsulation Human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and Nanoparticle of Conditioned Medium of AT-MSCs

Faried

Adam²,

Widowati³

et al. 2023

Trends Sci

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This study aims to characterize the microencapsulated type of human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and nanoparticles containing a conditioned medium of hAT-MSCs (CM-hATMSCs). The hAT-MSCs microencapsulation was measured the cells viability on 1st, 7th, and 14th days, by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethylphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, stability test was performed using magnetic stirrer at a speed of 300, 700, 1100, and 1200 rpm (revolutions per minutes) and applied on hAT-MSCs microencapsulation. The particle size of CM-hATMSCs nanoparticles were measured by particle size analyzer (PSA) and the growth factor levels namely Epidermal Growth Factor (EGF), Interleukin-6 (IL-6), Vascular Endothelial Growth Factor (VEGF), Insulin-Like Growth Factor-1 (IGF-1) were investigated using ELISA method. In the 14th day showed decreasing of cell viability percentage from 90 - 100% to 50% in both of 1.5×105 and 3×105 cells hAT-MSCs microencapsulation. CM-hATMSCs microencapsulation are quite stable and has a fairly good mechanical resistance when tested for stability using rotation at a speed of 1,200 rpm only damages 10 % of it. The CM-hATMSCs nanoparticles collected from hAT-MSCs growth medium in PSA test have an average particle size of 141 nm. Based on measurement of growth factor level, hAT-MSCs microencapsulation (3×105 cells) and CM-hATMSCs nanoparticles showed secretion of growth factors of EGF, IL-6, VEGF, and IGF-1 compared to positive controls. In summary, hAT-MSCs microencapsulation formulation using alginate showed good product based on the viability, stability, structure, and growth factor secretion ability. The characterization results showed that microencapsulated hAT-MSCs and CM-hATMSCs nanoparticles were successfully synthesized with the appropriate criteria. HIGHLIGHTS Stem cell therapy using Human ATMSCs (hAT-MSCs) has the potential to accelerate wound healing on the skin both directly and indirectly hMSC transplantation is affected by environment and inflammation which can trigger unwanted cell differentiation, so the solution is to use the nanoparticle method (CM-hATMSC) as a drug mobilization system hAT-MSCs and CM-hATMSCs display high secretion of growth factors namely EGF, IL-6, VEGF, and IGF-1 hAT-MSCs and CM-hATMSCs characterization was successfully synthesized according to the criteria GRAPHICAL ABSTRACT

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Stem Cell Therapy in Wound Healing and Tissue Regeneration

Cited by 7 publications

References 85 publications

Genipin Crosslinked Blended Collagen-Chondroitin: A Promising Biomaterial Scaffold Candidate for Cartilage Reconstruction

Genipin Crosslinked Blended Collagen-Chondroitin: A Promising Biomaterial Scaffold Candidate for Cartilage Reconstruction

Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review

Characterization of Microencapsulation Human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and Nanoparticle of Conditioned Medium of AT-MSCs

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