Growth and development of rabbit oocytes in vitro: Effect of fetal bovine serum concentration on culture medium

Sugimoto, Hironobu; Kida, Yutaka; Miyamoto, Yuki; Kitada, Kensaku; Matsumoto, Kazuya; Saeki, Kazuhiro; Taniguchi, Takeshi; Hosoi, Yoshihiko

doi:10.1016/j.theriogenology.2012.04.007

Cited by 11 publications

(14 citation statements)

References 38 publications

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“…1B). In our previous study, we confirmed that follicles < 399 mm in diameter collected from rabbit follicles did not possess competence to resume meiosis after IVM without in vitro follicle culture [28]. Follicles < 200 mm in diameter were too difficult to isolate without causing damage and could not be dissected for in vitro OCGC culture.…”

Section: Animal Use and Caresupporting

confidence: 51%

“…We recently developed a method for obtaining oocytes that develop to the blastocyst stage from rabbit ovarian follicles via in vitro follicle culture [28]; therefore, we attempted to utilize this system for establishing ESCs. First, we determined the pluripotency of cells in rabbit ICMs following immunosurgical isolation using qRT-PCR for pluripotency-related genes.…”

Section: Discussionmentioning

confidence: 99%

“…No viable newborns have yet been reported from the in vitro culture of immature follicles from rabbits [28] and it is known that ICM pluripotency is a prerequisite for fetus formation. Although limited development to blastocyst stages has been reported for rabbit embryos, the pluripotency of embryonic ICM cells has not been confirmed in immature follicular cultures.…”

Section: Discussionmentioning

confidence: 99%

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Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles

Teramura

Sugimoto

Frampton

et al. 2013

Stem Cells and Development

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In mammalian ovaries, many immature follicles remain after the dominant follicles undergo ovulation. Here we report the successful production of rabbit embryonic stem cells (ESCs) from oocytes produced by in vitro culture of immature follicles and subsequent in vitro maturation treatment. In total, we obtained 53 blastocysts from oocytes that received intracytoplasmic sperm injection followed by in vitro culture. Although only weak expression of POU5f1 was observed in the inner cell masses of in-vitro-cultured follicle-derived embryos, repeated careful cloning enabled establishment of 3 stable ESC lines. These ESC lines displayed the morphological characteristics of primed pluripotent stem cells. The ESC lines also expressed the pluripotent markers Nanog, POU5f1, and Sox2. Further, these ESCs could be differentiated into each of the 3 different germ layers both in vitro and in vivo. These results demonstrate that immature follicles from rabbits can be used to generate ESCs. Moreover, the use of rabbit oocytes as a cell source provides an experimental system that closely matches human reproductive and stem cell physiology.

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Section: Animal Use and Caresupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles

Teramura

Sugimoto

Frampton

et al. 2013

Stem Cells and Development

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“…Some previous research studied aspects of in vitro culture conditions for rabbit oocytes such as the culturing time, medium compositions, gas atmosphere and temperature (Sugimoto et al,2012). Many scholars have studied the age difference and the superovulation method and found that the in vitro maturation rates of oocytes from different age groups were different.…”

Section: Introductionmentioning

confidence: 99%

Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

Zhang

Cheng

et al. 2017

World Rabbit Sci.

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<p>To optimise the use of juvenile in vitro embryo transfer technologies in young rabbit females, superovulation was performed in New Zealand White young rabbit females at different ages and the expression mode of follicle-stimulating hormone receptor (FSHR) was explored using real-time quantitative polymerase chain reaction, and in vitro maturation (IVM) together with fertilisation (IVF) was conducted immediately after superovulation. The results showed that (1) the age factor significantly affected superovulation in young rabbit females, with 60 d as an optimal age; (2) the mRNA level of FSHR exhibited a rising trend, though it was lower at 30 to 40 d of age; (3) the maturation rate of the oocytes from 60 d old rabbits was significantly higher than in those from 50 d old rabbits; (4) the fertilisation rate of oocytes was not significantly different among rabbits 50, 60 and 70 d old.</p>

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“…Cryopreserved oocytes at germinal vesicle state (GV) might overcome the problem of damage in meiotic spindle followed by associated microtubule depolymerisation in matured cryopreserved oocytes (MII) (Aman and Parks 1994;Shaw et al, 2000; because the meiotic spindle is not organised yet . However, although rabbit oocytes are widely used as an in vitro model in experimental procedures on mammalian oocytes and embryos, to date there is little information about production of oocytes able to undergo successful maturation (Arias-Alvarez et al, 2010;Sugimoto et al, 2012). Regarding IVF, early works done in rabbit provided a background to facilitate the spread of IVF technology to other mammalian species (Chang 1959;.…”

Section: Referencesmentioning

confidence: 99%

Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes

Trigos¹,

Estrella²

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The general aim of this thesis was to optimise the current methodologies of oocyte cryopreservation in order to obtain live offspring from cryopreserved rabbit oocytes. In chapter 1, meiotic spindle configuration, cortical granules (CGs) distribution ABBREVIATIONS XV ABBREVIATIONS µg microgram µL microliters 6-DMAP 6-dimethylaminopurine AI Artificial insemination ART Assisted reproductive technologies ATP Adenosine triphosphate BM Base medium BSA Bovine serum albumin CaCl2 Calcium chloride Cai intracellular calcium CCD Charged-couple device CLC Cholesterol-loaded-cyclodextrin cm centimeter CO2 Carbon dioxide DMSO Dimethyl sulphoxide DNA Deoxyribonucleic acid DPBS Dulbecco's phosphate-buffered saline without calcium chloride EG Ethylene glycol ET Embryo transfer FBS Foetal bovine serum FITC Fluorescein isothiocyanate FITC-LCA Lens culinaris agglutinin labelled with fluorescein isothiocyanate GCs Cortical granules Analysis of development after parthenogenetic activation…………………………………………………..………………..84 4.4. Discussion……………..…………………………………………………………..85 4.5. References……………..………………………………………………………...87 5. CHAPTER III: Treatment with cholesterol-loaded methyl-β-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes……………………………………………….93 5.

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Growth and development of rabbit oocytes in vitro: Effect of fetal bovine serum concentration on culture medium

Cited by 11 publications

References 38 publications

Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles

Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles

Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes

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