Sourgrass (Digitaria insularis) is one of the main weeds in the soybean crop. In order to control its growth, an increase of herbicide rates is required to simplify its management as it a plant with high vegetative capacity and seed production. It implies to select the herbicide-resistent Digitaria insularis biotypes. Nevertheless, some information is still contrasting the antagonist of synthetic auxinic herbicides, associated with glyphosate and ACCase inhibitors mixtures, for the control of weeds resistant or tolerant to herbicides. This study aimed to evaluate the D. insularis control, with a mixture of herbicides applied in soybean pre-emergence, with sequential application in soybean post-emergence, and to check possible antagonism between ACCase inhibitors herbicides with synthetic auxins and other latifolicides. The experiment was conducted in Palotina, Paraná (Brazil) and Corpus Christi, Canindeyú, (Paraguay.) The treatments consisted of associations of glyphosate, ACCase inhibitors (clethodim, haloxyfop), and latifolicides (2,4-D, triclopyr, dicamba, carfentrazone, saflufenacil, chlorimuron). A randomized block design was used. Only in Palotina, the weed control was satisfactory after sequential application in post-emergence. An antagonism for all synthetic auxins was observed with glyphosate+clethodim or haloxyfop mixtures, in both locations. As a result, in Palotina an efficacious control of perennial D. insularis was found in pre-emergence burndown for some mixtures such as glyphosate+ACCase inhibitor added to carfentrazone, saflufenacil, or chlorimuron. Antagonism was observed for all synthetic auxins, in both locations. In Corpus Christi, the herbicide associations were not effective, even with the postemergence application in soybean of glyphosate+clethodim. With ineffective control for treatments composed with synthetic auxins, the post-emergence application in soybean increased the weed control with satisfactory final controls for all treatments.