Growth and metabolic response of whiteleg shrimp Litopenaeus vannamei and Nile tilapia Oreochromis niloticus in polyculture fed with potential probiotic microorganisms on different schedules

Abstract

Probiotics and co-culture of shrimp and tilapia are two strategies to improve yield and ecoefficiency of both species. However, only few studies have analyzed the combination of these two strategies. This study analyzes the effect of a mixture of potential probiotics supplied in the diet at different frequencies in a system of shrimp (10/m2) and tilapia (5/m2) in a trial lasting 84 days. The probiotics mixture was composed of four lactic acid bacteria and one yeast strain that were supplied either daily, every 5 days, or every 10 days in the diet and compared with a control without any supply of probiotics. At the end of the experiment, blood chemistry and hematology of shrimp and tilapia were analyzed as an index of physiological condition. Water quality did not differ between treatments. The final weight, feed conversion ratio, and yield of shrimp were significantly higher with daily supply of probiotics compared to shrimp that did not receive probiotics supply, with intermediate values for treatments with probiotics supply every 5 or 10 days. However, level of supplementation every 5 days could be considered as optimal because no significant differences with daily supply were observed for any variable, whereas the supply each 10 days resulted in a significantly lower yield. Significantly higher final weight, feed conversion ratio, and yield of tilapia occurred with daily supplements. Blood cholesterol in tilapia significantly decreased with increasing frequency of probiotics supplementation. These results indicate that probiotics supplements for shrimp and tilapia cultivated simultaneously improved yields and physiological condition.

Lupin kernel meal as fishmeal replacement in formulated feeds for the Whiteleg Shrimp (Litopenaeus vannamei)

“…Acylglyceride (AG) levels in the haemolymph of shrimp fed the commercial diet (240 mg dl −1 ) and the L10 diet (180 mg dl −1 ) are much higher than levels from other studies (Apún-Molina et al, 2015;Mercier et al, 2006). This might be partly explained by the acylglyceride content measured including the acylglyceride content of lysed haemocyte cells, as for the other heamolymph metabolites due to sample treatment.…”

Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor)

“…These eels were fed a paste prepared from a 1:2.2 powdered feed to water ratio. Based on earlier studies (Aisyah et al, 2020;Apún-Molina et al, 2015), a new culture of Bacillus sp. PCP1, Lactococcus sp.…”

Multistrain probiotics use in main commercially cultured freshwater fish: a systematic review of evidence

“…freundii, Candida parapsilosis, Pseudomonas spp. (with P. aeruginosa highly represented), Micrococcus luteus, Lactobacillus plantarum, L. fermentum, L. acidophilus, L. brevis, L. rhamnosus, L. lactis, Pediococcus pentosaceus and P. parvulus (Aly et al 2008a;El-Rhman et al 2009;Giri et al 2014;Ap un-Molina et al 2015;Ridha & Azad 2016;Maji et al 2017;Amir et al 2018;Van Doan et al 2018;Xia et al 2018). Giri et al (2014) showed that a mixture of B. subtilis, P. aeruginosa and L. plantarum isolated from the gut of roho labeo enhanced growth, immune response and survival after A. hydrophila challenge in juvenile L. rohita.…”

“…Similarly, Apún‐Molina et al . (2015) showed that four strains of the LAB, Ped. parvulus and the yeast Candida parapsilosis , all isolated from Nile tilapia intestine, enhanced growth.…”