This study examined the effect of potential probiotic bacteria on growth and survival of the tilapia Oreochromis niloticus, under high density and suboptimum temperature. Presumptive Bacillus and lactic acid bacteria (LAB) were isolated from tilapia and from their culture system and were characterized for haemolytic and enzymatic activity, and antagonism against Vibrio. Selected strains were included in the diet of juvenile tilapia and evaluated during a 134‐day assay. The experiment was conducted with four treatments: (1) fish fed with commercial feed plus Dry Oil®; (2) fish fed with commercial feed plus LAB; (3) fish with bacilli in water; (4) fish with a mixture of treatments 2 and 3. Tilapias in all treatments, including bacteria, grew significantly better than fish fed with commercial feed plus Dry Oil® (control group). Survival was similar in all treatments. The physicochemical parameters of the culture system were maintained within the optimal ranges for the species, with the exception of temperature (19.9–24.82 °C). Animals fed diet supplemented with bacilli and LAB had good survival and the best growth performance, suggesting that bacteria are appropriate growth‐stimulating additives in tilapia cultivation.

No abstract

Consumer demand for aquatic animals has led to increased interest in using new species for aquaculture purposes. This study appraises the potential for aquaculture of the cauque prawn Macrobrachium americanum using wild juveniles from the west coast of Mexico. Four treatments were applied; treatments A and B consisted of 196 prawns/m2 and treatments C and D of 98 prawns/m2, with and without shelter, respectively. Growth and survival under these conditions were examined for 84 d. The combination of 98 prawns/m2 and shelter resulted in the best survival and growth, whereas the poorest performance was in the treatment with 196 prawns/m2 and no shelter.

No abstract

Trypsin from Pacific white shrimp Litopenaeus vannamei is a polymorphic protein composed of three alleles (A, B, and C). The C allele is monomorphic for the first region, while A and B are allelic variations for the second region that are segregated according to Mendelian rules. These three alleles give rise to three individual trypsin phenotypes displayed in sodium dodecyl sulfate polyacrylamide gel electrophoresis: CBA, CB, and CA. The expected frequencies from mating CBA males and females resulted in offspring that consisted of 25% CB, 25% CA, and 50% CBA trypsin phenotypes. To define a management protocol for studying shrimp performance associated with the trypsin phenotype, the goal of this study was to determine if the expected proportions of the three trypsin phenotypes are represented in the population structure at breeding age in 11 hatcheries. Here, we hypothesized that the population structure of the trypsin phenotypes is represented by frequencies that are subject to selection, and it is possible to establish a management protocol to study shrimp performance. A total of 2,828 breeding shrimp from 11 hatcheries were analyzed to describe their population structure. The results showed a high frequency of CBA breeders and a near absence of the CA phenotype in the population structure. To discard the possibility that man‐made selection is the cause of the trypsin phenotype frequency, the offspring of CBA breeders were cultured in nine commercial ponds, and the phenotype frequencies were monitored during 75 d. Interestingly, the CA phenotype was only present during the early stages of culture, which suggests that the population structure is not due to human selection of CBA shrimp in the hatcheries. In this condition, poslarvae production could be directed to make trials that contribute to describe the difference between the culture performance between phenotypes, but not to support the commercial production of larvae with the highest culture performance trypsin phenotype.