Growth and Persistence of Campylobacter jejuni in Foodstuffs

Stetsenko, V V; Efimochkina, N R; Pichugina, T V

doi:10.1007/s10517-019-04435-x

Cited by 13 publications

(9 citation statements)

References 3 publications

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“…Additionally, the manifestation of campylobacteriosis in humans is dependent on the virulence of the infecting strain and on the health of the host. Although Campylobacter lacks classical virulence factors, such as endo- or enterotoxins, colonisation and survival determinants associated with motility, adhesion, invasion, capsule and biofilm synthesis, cytolethal distending toxin synthesis, molecular mimicry and secretion systems can result in the manifestation of more severe illness and persistence in the environment ( Bolton, 2015 ; Stetsenko and Efimochkina, 2018 ; Wysok et al, 2020 ). Therefore, a more in-depth understanding of the changes in strains, virulence and antimicrobial resistance during broiler production would facilitate a more accurate assessment of the risk to public health associated with the broiler rearing cycle.…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterisation of Campylobacter jejuni Isolates Recovered During Commercial Broiler Production

et al. 2021

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Background:Campylobacter is commonly transmitted to humans from chickens. Campylobacter jejuni is the species most frequently associated with human illness, and the most prevalent species recovered from poultry.Objective: The objective of this study was to analyse a sub-population of C. jejuni from two broiler flocks on the farm and at slaughter using whole-genome sequencing to gain insights into the changes in the Campylobacter population during broiler production, including changes in virulence and antimicrobial resistance profiles.Methods: In this study, ten composite faecal samples (n=10), obtained by pooling ten fresh faecal samples (n=10), were collected in the broiler house on two farms on days 14, 21, 28, and 34 (n=80) and ten composite (n=10) caecal samples were collected at the time of slaughter for each flock (n=20). These were tested for C. jejuni using the ISO 10272-2:2016 method. Seven isolates were randomly selected from each of the nine Campylobacter-positive sampling points (n=63) and were subjected to antimicrobial susceptibility tests. Their genomes were sequenced and the data obtained was used to characterise the population structure, virulence, antimicrobial resistance determinants and inter-strain variation.Results: The Farm 1 isolates had three MLST types (ST257-257, ST814-661 and ST48-48) while those on Farm 2 were ST6209-464 and ST9401. Interestingly, only the MLST types positive for most of the virulence genes tested in this study persisted throughout the production cycle, and the detection of antimicrobial resistance determinants (gyrA T86I and tetO) increased after thinning and at slaughter, with the detection of new strains.Conclusion: The persistence of the most virulent strains detected in this study throughout the production cycle has important implications for the risk to consumers and requires further investigation. The detection of new strains within the population corresponding with the time of thinning and transportation reflects previous reports and provides further evidence that these activities pose a risk of introducing new Campylobacter strains to broiler batches.

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Section: Introductionmentioning

confidence: 99%

Genomic Characterisation of Campylobacter jejuni Isolates Recovered During Commercial Broiler Production

et al. 2021

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“…Planktonic C. jejuni is extremely susceptible to multiple stressors (Lee et al, 2019;Oh et al, 2019), especially to oxygen pressure in the environment, as aerotolerance factors heavily in the transmission of C. jejuni to humans via foods under aerobic conditions (Euna et al, 2015;Oh et al, 2018). C. jejuni can be observed in a viable but non-culturable (VBNC) state in vitro (Magajna and Schraft, 2015;Stetsenko et al, 2019). However, it is easy to acquire C. jejuni from water, soil, and so on (Teh et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuni Biofilm Formation Under Aerobic Conditions and Inhibition by ZnO Nanoparticles

Zhong

Zhang

et al. 2020

Front. Microbiol.

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Campylobacter jejuni is a major foodborne pathogen worldwide. As it forms biofilms, it can become a persistent contaminant in the food and pharmaceutical industries. In this study, it was demonstrated that C. jejuni could make more biofilm in aerobic conditions than in microaerobic conditions, and only 13.9% C. jejuni entered coccus (a VBNC state) under microaerobic conditions; however, the rate increased to 95.5% under aerobic conditions. C. jejuni could form more biofilm in mixed culture with Escherichia coli or Pseudomonas aeruginosa than in pure culture. Scanning electron microscope results showed that C. jejuni retained its normal spiral shape under aerobic conditions for 48 h by forming crosslinks with the aerobic and facultative anaerobic bacteria. Additionally, culture medium containing 0.5 mg/ml ZnO nanoparticles inhibited biofilm formation. Our results provide information on a new approach to controlling contamination via C. jejuni.

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“…Oxygen saturation may also be a key factor in biofilm formation where, under aerobic conditions, microaerophilic C. jejuni demonstrates a marked increase in the amount of biofilm formed, suggesting biofilm formation to be a mechanism by which C. jejuni withstands oxidative stress [24,51]. In addition, the ability of C. jejuni cells to adhere to surfaces has been found to markedly increase under oxygenated conditions [52], and biofilms formed under oxygenated environments appear to have higher biomass than those formed in microaerobic environments [53,54].…”

Section: Metabolism and Environmental Factorsmentioning

confidence: 99%

Bridging the Gap: A Role for Campylobacter jejuni Biofilms

2020

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Campylobacter jejuni is the leading cause of bacterial gastroenteritis in the developed world. Cases of Campylobacteriosis are common, as the organism is an avian commensal and is passed on to humans through contaminated poultry meat, water, and food preparation areas. Although typically a fastidious organism, C. jejuni can survive outside the avian intestinal tract until it is able to reach a human host. It has long been considered that biofilms play a key role in transmission of this pathogen. The aim of this review is to examine factors that trigger biofilm formation in C. jejuni. A range of environmental elements have been shown to initiate biofilm formation, which are then affected by a suite of intrinsic factors. We also aim to further investigate the role that biofilms may play in the life cycle of this organism.

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Growth and Persistence of Campylobacter jejuni in Foodstuffs

Cited by 13 publications

References 3 publications

Genomic Characterisation of Campylobacter jejuni Isolates Recovered During Commercial Broiler Production

Genomic Characterisation of Campylobacter jejuni Isolates Recovered During Commercial Broiler Production

Campylobacter jejuni Biofilm Formation Under Aerobic Conditions and Inhibition by ZnO Nanoparticles

Bridging the Gap: A Role for Campylobacter jejuni Biofilms

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