Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L.

Abstract

Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics showed that a change in the medium caused shifts in peaks of growth and production, and modifications of the cell metabolism. RA production can be correlated with growth or begins only when growth has stopped.

In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds

“…The amounts were twice as high as that in roots of both naturally growing and in vitro regenerated plants (Table 1). It was earlier demonstrated, that RA was produced in in vitro cultures of broad range of plant species including cultures of S. officinalis, in which RA concentration varied from 1.4% up to 36% of dry weight, after optimization of culture conditions (Whitaker et al 1984;Hippolyte et al 1992). On the other hand, Gomes et al (2002) reported a maximum production of 2.8 mg g -1 RA of dry weight from the sage shoot culture i.e.…”

“…According to observations of many investigators and the results of our work it has been shown that accumulation of RA is not determined by a degree of culture differentiation. The ability to synthesize RA by in vitro cultures is rather dependent on plant species, cell line and its age (Whitaker et al 1984;Hippolyte et al 1992;Del Bano et al 2003). This is the case with sage cell suspension obtained in this work, which after seven passages produced 13.7 mg g -1 of RA on the basis of dry weight.…”

“…However, only a few studies have reported on production of compounds with antioxidant activity in in vitro culture of Salvia officinalis. Hippolyte et al (1992) reported the accumulation of RA in cell suspension culture of this species. Phenolic compounds with antioxidant activity have been found in shoot culture of S. officinalis by Santos-Gomes et al (2002).…”

Growth and Phenolics Production of Cell Suspension Culture ofLavandula Vera MM

“…Cultivation was performed in Erlenmeyer flasks with 1/5 net volume on the shaker (11,6 rad.sec-1 ) for 12 days. For inoculation 20 % (V IV) of the respective cell suspension was used, cultivated under the described conditions for 7 days.…”

“…Extraction of phenolic compounds. The cell and culture medium were separated by filtration, lyophilised and e},.1facted with methanol according Hippolyte et al (6). The methanol extract was concentrated under vacuum to the appropriate volume and was used to follow the time course of phenolics formation and to identify the main phenolics, synthesised and secreted from Lavandula vera-MM.…”