Growth and Starvation of a Strain of Klebsiella  pneumoniae Isolated from a Brazilian Oil Formation

Bossolan, Nelma Regina Segnini; Godinho, Mirna Januária Leal; Volpon, Antonia Garcia Torres

doi:10.1007/s11274-005-6811-7

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…The miniaturization of cells increased the surface/volume ratio of the cells, facilitating the assimilation of nutrients from a nutrient pooor environment (Amy and Morita 1983). After being submitted to starvation, Klebsiella pneumoniae cells presented a change of shape from rod to coccoid or coccobacillus (Bossolan et al 2005a), Shewanella alga BrY cells presented a reduction of 50% in volume and cell roundness (Caccavo et al 1996), actinomycete p-nitrophenol-degrading cells presented filament fragmentation in cells of 1.7 · 0.7 lm (Herman and Costerton 1993), and freshwater isolates presented change of shape from rod or vibrio to coccoid and reduction in cell size between 40 and 79% (Amy and Morita 1983).…”

Section: Resultsmentioning

confidence: 99%

Starvation survival of Gordonia polyisoprenivorans CCT 7137, isolated from contaminated groundwater in Brazil

Fusconi

Godinho

Bossolan

2007

World J Microbiol Biotechnol

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Gordonia polyisoprenivorans CCT7137, exopolysaccharide-producing bacterium, was isolated from groundwater contaminated with leachate in a former municipal landfill site (São Paulo, Brazil). The strain was submitted to starvation in phosphate-buffered saline solution for 56 days so as to evaluate its behavior regarding cuturability and cell morphology. As a response to starvation, G. polyisoprenivorans CCT7137 presented reduction in viable cell count, cell size and cell shape alteration. The initial number of viable cells was 1.51 · 10 7 c.f.u. ml. After 7 days of starvation culturability dropped to 13.70% (2.07 · 10 6 c.f.u/ml) and, after 56 days, to 3.25% (4.93 · 10 5 c.f.u/ml). It was also observed that after 7 days of starvation the cell size presented an average reduction in the values of length, length/width ratio, volume and area of 50, 58, 40 and 42%, respectively. The length/width ratio showed a change of shape from rod to coccobacillus. Changes in cell dimensions and distribution of cell into classes were not significant after day 7 of starvation. The results obtained show that it is not necessary for the strain to starve for more than a week to obtain G. polyisoprenivorans CCT7137 size-reduced cells. The results also indicate the potential for its starved forms to be used in future tests in porous medium to study the production of exopolysaccharide in situ.

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Section: Resultsmentioning

confidence: 99%

Starvation survival of Gordonia polyisoprenivorans CCT 7137, isolated from contaminated groundwater in Brazil

Fusconi

Godinho

Bossolan

2007

World J Microbiol Biotechnol

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“…Similarly, our twomonth long starvation experiments revealed no differences in survival between capsulated and non-capsulated strains. Previous reports had already shown that in the long term, Klebsiella spp are very resilient and have very low death rates (Bossolan et al 2005, Lappin-Scott et al 1988, including in lake water at temperatures close to 0 (Niemela and Vaatanen 1982) or during long-term starvation in PBS as a member of an oral community (Baker et al 2019).…”

Section: R Ementioning

confidence: 99%

Selection for the bacterial capsule in the absence of biotic and abiotic aggressions depends on growth conditions

Nucci

Rocha

Rendueles

2020

Preprint

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Extracellular capsules are the outermost layer of some cells and are prevalent across Bacteria. They protect the cell against both abiotic and biotic stresses such as bacteriophages and the host immune system. Yet, it is unclear if capsules contribute to fitness in the absence of external aggressions, in spite of the cost of production. Here, we enquire if there are conditions favouring the presence of the capsule in Klebsiella, where it is a major virulence factor. We allowed independent populations of 19 different strains, representing six different capsule serotypes, to evolve for twenty generations in different growth media. We observed that capsules were maintained in nutrient-poor media but were selected against in nutrient-rich media. Competitions with capsule mutants in nine different strains showed that capsules provide ca 20% of fitness advantage in nutrient poor-conditions. This is not caused by differences in maximal growth rate, nor death rates. We showed that most capsulated populations reached higher yields. We further characterized how growth conditions affected the role of the capsule during pathogenensis and whether this is conserved across Klebsiella strains. Our data reveals that the environment and the serotype strongly influences major virulence traits such as hypermucoviscosity and biofilm formation. Our data shows that the capsule can be selected for in situations lacking abiotic, but also biotic stresses. This raises the possibility that the forces selecting for the presence of a capsule are different from the ones selecting for rapid serotype variation.IMPORTANCEBacterial capsules are a wide-spread virulence factor that limits efficacy of antimicrobial therapy. Whereas most studies focus on the role of the capsule in pathogenesis, very few have addressed the conditions under which the capsule is primarily selected for. Here, we show that small changes in growth media have a strong impact in the maintenance of the capsule and the fitness advantage they confer. Our results suggest that the forces selecting for the capsule may be different from those selecting for its composition diversification, namely the immune system and phage predation. Our results further support that the role of the capsule in virulence may be a by-product of adaptation, hinting that there may be other functionalities yet to be discovered for it.

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Adaptation of Shigella flexneri to starvation: morphology, outer membrane proteins and lipopolysaccharide changes

et al. 2017

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Growth and Starvation of a Strain of Klebsiella pneumoniae Isolated from a Brazilian Oil Formation

Cited by 6 publications

References 22 publications

Starvation survival of Gordonia polyisoprenivorans CCT 7137, isolated from contaminated groundwater in Brazil

Starvation survival of Gordonia polyisoprenivorans CCT 7137, isolated from contaminated groundwater in Brazil

Selection for the bacterial capsule in the absence of biotic and abiotic aggressions depends on growth conditions

Adaptation of Shigella flexneri to starvation: morphology, outer membrane proteins and lipopolysaccharide changes

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