Growth and Steviol Glycoside Content of Stevia rebaudiana Bertoni in the Thin-Layer Liquid Culture Treated with Late-Stage Gibberellin Biosynthesis Inhibitors

Saptari, Rizka Tamania; Esyanti, Rizkita Rachmi; Putranto, Riza Arief

doi:10.1007/s12355-019-00745-6

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…Total stevioside and rebaudioside A yield from stevia after six weeks of incubation in TIB with Daminozide treatments. Different letters in each parameter indicate means are signi cantly different according to Tukey's post hoc test at α = 0.05, n = 5.The result was slightly different from those reported bySaptari et al (2020) that applied Daminozide in thin layer liquid culture, in which 10 ppm and 20 ppm Daminozide treatments exhibited similar performance in modulated stevioside and rebaudioside A yields. It indicates speci c optimal condition of Daminozide treatment is required for each culture type.…”

contrasting

confidence: 71%

“…Nevertheless, having different stage of pathway in blocking GA biosynthesis, application of Daminozide in stevia was more bene cial since it also potentially increases SG yield (Yoneda et al 2018;Saptari et al 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Daminozide has similar chemical structure with 2-oxoglutaric acid (2OG) thus can act competitively with 2OG in binding with 2ODD enzymes which catalyze GA late-stage biosynthesis (Rademacher 2000). Interestingly, the use of retardants is also corelated with the increment of plant yield (Karimi et al 2014;Saptari et al 2020). Accordingly, this study aimed to provide protocols for in vitro propagation of stevia with high biomass yielding and SG content by chemically manipulating SG and GA pathways using Daminozide treatment in TIB.…”

Section: Introductionmentioning

confidence: 99%

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Daminozide enhances the vigor and steviol glycoside yield of stevia (Stevia rebaudiana Bert.) propagated in temporary immersion bioreactors

Saptari

Esyanti

Putranto

2022

Plant Cell Tiss Organ Cult

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Stevia (Stevia rebaudiana Bertoni) contains sweet compound widely used as natural sweetener, steviol glycoside (SG). SG is a diterpenoid secondary metabolite synthesized from ent-kaurenoic acid, the same precursor of Gibberellin (GA). Therefore, in this study, a GA inhibitor, Daminozide (0, 10, 20 ppm) was used to block ent-kaurenoic acid conversion towards GA synthesis in attempt to increase SG content of stevia propagated in Temporary Immersion Bioreactor (TIB). Daminozide in 10 mg/L was observed to be the optimum concentration which increased biomass weight and SG content (stevioside and rebaudioside A) up to 40%. The treatment also increased transcripts accumulation of genes enrolled in SG biosynthesis, such as SrKA13H, SrUGT85C2, and SrUGT76G1, indicating SG pathway become more active due to the inhibition of GA pathway. Furthermore, the inhibition of GA was also indicated by the upregulated expression of GA biosynthesis gene (GA3ox) as the result of feedback regulation, and the downregulated expression of GA catabolism gene (GA2ox2) as the result of feed-forward regulation caused by inhibitor treatment. Key MessageThis study reports data regarding the in uence of inhibition to the late-stage of GA biosynthesis by chemical treatment in modulating SG yield from stevia in vitro culture; SG and GA pathways are related.

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contrasting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Daminozide enhances the vigor and steviol glycoside yield of stevia (Stevia rebaudiana Bert.) propagated in temporary immersion bioreactors

Saptari

Esyanti

Putranto

2022

Plant Cell Tiss Organ Cult

Self Cite

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“…The extracts was incubated in a water bath at 70ºC for 30 min and evaporated with a rotary vacuum evaporator until all solvents evaporated [12].…”

Section: 𝑥 100%mentioning

confidence: 99%

“…The stationary phase was a C-18 column (250 x 4.6 mm; 5μm) with a protective column. The flow rate was 0.5 mL/min and separation were detected using a 210 nm UV detector [12]. The extract (1μL) was injected using Hamilton Syringe Model 702 N SYR injectors.…”

Section: 𝑥 100%mentioning

confidence: 99%

The effect of methyl jasmonate on growth, stevioside and rebaudioside A production in Stevia rebaudiana shoot culture using mist bioreactor

Rahmawati,

Saksyasamita,

Emmanuel

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Steviol glycosides (SG) from Stevia rebaudiana have been used as natural sweeteners to substitute sucrose. SG production naturally needs large biomass. Therefore, it requires large area of cultivation. In vitro cultivation can be used as an alternative to propagate Stevia, as well as for increasing the production of SG. In this experiment, we conducted Stevia propagation in bioreactor and induced SG production through methyl jasmonate (MeJa) elicitation. The objective of this study was to evaluate the effect of MeJA on plant growth and SG contents in Stevia shoot cultivated using mist bioreactor. Elicitation was carried out by MeJa spraying for 30 min every 6 h. In treatment A, we used 10 μM MeJa for 72 h and 100 μM MeJa for 24 h for treatment B. The results showed that treatment A gave higher increase in shoot growth (485%), biomass production (547%), and relative growth rate (0.13) compared to treatment B. There was no significant difference in stevioside content among treatments (A and B) and controls, meanwhile treatment B produced rebaudioside A (0.0113 mg/g) higher than treatment A (0.0062 mg/g). We concluded that treatment A was better in inducing growth and treatment B could be used to increase rebaudioside A.

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Biotechnological interventions of in vitro propagation and production of valuable secondary metabolites in Stevia rebaudiana

Shahnawaz

Pandey

Konjengbam

et al. 2021

Appl Microbiol Biotechnol

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Growth and Steviol Glycoside Content of Stevia rebaudiana Bertoni in the Thin-Layer Liquid Culture Treated with Late-Stage Gibberellin Biosynthesis Inhibitors

Cited by 7 publications

References 40 publications

Daminozide enhances the vigor and steviol glycoside yield of stevia (Stevia rebaudiana Bert.) propagated in temporary immersion bioreactors

Daminozide enhances the vigor and steviol glycoside yield of stevia (Stevia rebaudiana Bert.) propagated in temporary immersion bioreactors

The effect of methyl jasmonate on growth, stevioside and rebaudioside A production in Stevia rebaudiana shoot culture using mist bioreactor

Biotechnological interventions of in vitro propagation and production of valuable secondary metabolites in Stevia rebaudiana

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