Growth and transcriptional effect of dietary 2,2′,4,4′-tetrabromodiphenyl ether (PBDE-47) exposure in developing zebrafish (Danio rerio)

Chen, Te-Hao; Cheng, Ying-Ming; Cheng, Jing-O; Chou, Chun-Ting; Hsiao, Yu-Chen; Ko, Fung-Chi

doi:10.1016/j.ecoenv.2009.12.033

Cited by 29 publications

(20 citation statements)

References 54 publications

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“…Previous studies have also found slight cyp1a induction following PBDE exposure in rats . Furthermore, cyp1a1 was weakly induced at 38 and 60 d postfertilization in zebrafish, suggesting constant upregulation over the duration of exposure during development . Exposure to BDE 183 upregulated cyp1a1 and cyp1b1 despite its relatively low uptake compared with other congeners in this study.…”

Section: Resultssupporting

confidence: 70%

“…2). In a previous study, dio1 and dio2 were induced in juvenile zebrafish exposed to the technical pentamixture DE-71 [28]; however, in a study by Chen et al [29], neither deiodinase was induced by BDE 47 after 38 d of feeding exposure in zebrafish. Results may differ due to differences in route of exposure, dose, and age at assessment.…”

Section: Gene Transcriptionmentioning

confidence: 80%

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Uptake and Metabolism of Individual Polybrominated Diphenyl Ether Congeners by Embryonic Zebrafish

Usenko

Robinson

Bruce

et al. 2013

Environmental Toxicology and Chemistry

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Embryonic zebrafish were used to compare the uptake and metabolism of six polybrominated diphenyl ether (PBDE) congeners (BDEs 28, 47, 99, 100, 153, and 183) and identified metabolites from static exposures at 24 and 120 h postfertilization (hpf). An inverse relationship was observed between uptake of PBDEs and their octanol-water partitioning coefficients (uptake of BDEs 28 and 47>99 and 100>153 and 183). Debromination metabolites were identified in all congeners (excluding BDE 28) tested in the 120-hpf tissue samples. Interestingly, BDE 153 underwent meta-debromination, forming BDEs 47 and 99. Gene transcription analysis was conducted at 120 hpf to identify potential metabolic pathways for the PBDEs examined in the present study (gstpi, deiodinases 1 and 2, cyp1a1, cyp1b1, and ugt5g). The greatest induction was of ugt5g for all congeners and deiodinase transcription was also upregulated by BDEs 28, 47, and 183. The cyp1a1 and cyp1b1 were upregulated by BDEs 28, 47, 99, and 183. The least alterations in gene transcription were in the BDE 153-exposed embryos. A clear primary pathway of debromination metabolism was not identified; however, upregulation of these different genes indicated that fish were responding to exposure of PBDEs. Furthermore, the present study demonstrated that the most bioavailable congeners are also those with the highest reported toxicity.

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Section: Resultssupporting

confidence: 70%

Section: Gene Transcriptionmentioning

confidence: 80%

Uptake and Metabolism of Individual Polybrominated Diphenyl Ether Congeners by Embryonic Zebrafish

Usenko

Robinson

Bruce

et al. 2013

Environmental Toxicology and Chemistry

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“…To date, PBDE studies have not found a histopathological effect in adult fish. Several studies in fish have also evaluated alterations in gene expression following exposure to BDE 47 or to DE-71, primarily focusing on genes related to the hypothalamic-pituitary-thyroid axis [14,21,22].…”

Section: Introductionmentioning

confidence: 99%

PBDE developmental effects on embryonic zebrafish

Usenko

Robinson

Usenko

et al. 2011

Environmental Toxicology and Chemistry

103

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Polybrominated diphenyl ethers (PBDEs) have become ubiquitous environmental contaminants with potential for bioaccumulation and maternal-fetal transfer that has led to regulatory bans and/or phasing out of several technical mixtures of PBDEs. In the present study, six PBDE congeners (BDE 28, BDE 47, BDE 99, BDE 100, BDE 153, BDE 183) were evaluated for developmental effects on embryonic zebrafish. These congeners were chosen because they are environmentally relevant and cover a wide range of physical-chemical properties. Alterations in behavior, physical malformations, and mortality were scored daily until 168 h postfertilization (hpf). A concentration-dependent increase in spontaneous movement indicated an early onset of behavioral responses to PBDE exposures. Spontaneous movement was affected the most by BDE 47 and BDE 28, whereas BDE 183 did not alter behavior at any concentration tested. Swimming rates were significantly increased by BDE 28 at 96 and 120 hpf, but decreased swimming activity at 168 hpf. Additionally, BDE 47 significantly decreased the swimming rate at 168 hpf. Other endpoints included malformations and mortality. Congeners with fewer bromines (BDE 28, 47, 99, and 100) also induced a curved body axis starting around 120 hpf, which was followed by mortality. BDEs 153 and 183, however, did not elicit these adverse effects. A relationship was found between log K(OW) and median lethal concentration (LC50) and median effective concentration (EC50). Structure-activity relationships in this study suggest that PBDE acute toxicity results from a receptor-mediated effect and further studies are necessary to determine these pathways.

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“…Specifically, dose-dependent increases in relative mRNA transcripts encoding vitellogenin (Vtg; egg yolk precursor protein) and zona radiata protein (eggshell protein) were measured in hepatocytes of juvenile male Atlantic salmon (S. solar) exposed to BDE-47 and a PBDE mixture (BDE-47, 153, -154). 226 However, some of these in vitro findings have not been reproduced with in vivo studies of juvenile Atlantic salmon exposed to the PentaBDE mixture 75 or in zebrafish larvae exposed to BDE-47, 95 whereby no change in the expression of Vtg mRNA transcript abundances were detected. Thus, while the in vitro evidence is limited because it may not account for the extensive and coordinated interactions among cells and tissues, conversely the in vivo research conducted to date targeting PBDE effects on male fish reproduction has only been in two species, targeting largely transcriptional changes in immature animals for just a limited number of genes.…”

Section: Pbde Effects On Male Fish Reproductionmentioning

confidence: 98%

“…In vivo and in vitro studies in fish have shown both weak induction 78,95 and inhibition 82,96 of ethoxyresorufin-O-deethylase (EROD) activity (biomarker of CYP1A and aryl hydrocarbon (AhR) induction) upon exposure to individual PBDE congeners. However, a larger number of PBDE studies in teleost fish have shown no AhR/CYP1A activation.…”

Section: Mixed Evidence Of Pbde Oxidative Metabolism In Fishmentioning

confidence: 99%