Growth Characteristics of Cultured Human Normal Bladder
Epithelial Cells:
A Comparison with Urothelial Carcinoma Cell Cultures

Logothetou-Rella, H; Nesland, J. M.; Karayiannis, A; Dimopoulos, C.

doi:10.1159/000473448

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…The ultrastructural features of normal and malignant urothelial cells were the same as those of cultivated cells originating in these tissues, described in earlier reports [2][3][4]. …”

Section: Electron Microscopysupporting

confidence: 72%

“…Of these recurrences, 20% will be deeply invasive or metastatic [1], An identification method of those low-Such cell cultures exhibited tumor nodules consisting of naked nuclei in a glycosaminoglycan (GSG) mass, intra cellular perinuclear and accumulated GSGs in sacs as well as membrane extensions [2,3]. These features were ob served in human embryonic cell cultures and were absent in normal urothelial cell cultures [4,5], This study aims to identify the observed cytologic fea tures of malignant bladder-cultivated cells in cell imprints and urine-exfoliated cells from patients with invasive bladder carcinoma. A common cytologic feature of the invasive bladder carcinoma identified in the cytology of low-stage bladder cancers could be useful to distinguish those superficial tumors which become invasive later on.…”

Section: Introductionmentioning

confidence: 99%

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A Cytologie, Ultrastructural and Immunocytochemical Comparison of Tumor Cells and Cell Cultures Originating in Invasive Bladder Carcinoma

Logothetou-Rella

Karayiannis²,

Vamvassakis³

et al. 1992

Eur Urol

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“…The ultrastructural features of normal and malignant urothelial cells were the same as those of cultivated cells originating in these tissues, described in earlier reports [2][3][4]. …”

Section: Electron Microscopysupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

A Cytologie, Ultrastructural and Immunocytochemical Comparison of Tumor Cells and Cell Cultures Originating in Invasive Bladder Carcinoma

Logothetou-Rella

Karayiannis²,

Vamvassakis³

et al. 1992

Eur Urol

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Primary Uroepithelial Cultures

Truschel¹,

Ruiz²,

Shul'man³

et al. 1999

Journal of Biological Chemistry

102

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Despite almost 25 years of effort, the development of a highly differentiated and functionally equivalent cell culture model of uroepithelial cells has eluded investigators. We have developed a primary cell culture model of rabbit uroepithelium that consists of an underlying cell layer that interacts with a collagen substratum, an intermediate cell layer, and an upper cell layer of large (25-100 m) superficial cells. When examined at the ultrastructural level, the superficial cells formed junctional complexes and had an asymmetric unit membrane, a hallmark of terminal differentiation in bladder umbrella cells. These cultured "umbrella" cells expressed uroplakins and a 27-kDa uroepithelial specific antigen that assembled into detergent-resistant asymmetric unit membrane particles. The cultures had low diffusive permeabilities for water (2.8 ؋ 10 ؊4 cm/s) and urea (3.0 ؋ 10 ؊7 cm/s) and high transepithelial resistance (>8000 ⍀ cm 2 ) was achieved when 1 mM CaCl 2 was included in the culture medium. The cell cultures expressed an amiloride-sensitive sodium transport pathway and increases in apical membrane capacitance were observed when the cultures were osmotically stretched. The described primary rabbit cell culture model mimics many of the characteristics of uroepithelium found in vivo and should serve as a useful tool to explore normal uroepithelial function as well as dysfunction as a result of disease.

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Growth Characteristics of Cultured Human Normal Bladder Epithelial Cells: A Comparison with Urothelial Carcinoma Cell Cultures

Cited by 2 publications

References 2 publications

A Cytologie, Ultrastructural and Immunocytochemical Comparison of Tumor Cells and Cell Cultures Originating in Invasive Bladder Carcinoma

A Cytologie, Ultrastructural and Immunocytochemical Comparison of Tumor Cells and Cell Cultures Originating in Invasive Bladder Carcinoma

Primary Uroepithelial Cultures

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