Growth control: brassinosteroid activity gets context

Singh, Amar Pal; Savaldi-Goldstein, Sigal

doi:10.1093/jxb/erv026

Cited by 120 publications

(70 citation statements)

References 99 publications

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“…Considering the phenotypes obtained using the chemical inhibitor in Brachypodium, we propose that Pcz treatments represent a powerful tool for studying BRs in this model plant. Pcz could be administered to investigate vascular and stomatal development, root growth, cell elongation, and meristematic cell maintenance, among other specific processes in which BR is known to play active roles [22, 23]. …”

Section: Discussionmentioning

confidence: 99%

Identification of brassinosteroid genes in Brachypodium distachyon

Corvalán

Choe

2017

BMC Plant Biol

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BackgroundBrassinosteroids (BRs) are steroidal phytohormones that are involved in diverse physiological processes and affect many important traits, such as plant stature, stress tolerance, leaf angle, fertility, and grain filling. BR signaling and biosynthetic pathways have been studied in various plants, such as the model dicot Arabidopsis thaliana; however, relatively little is known about these pathways in monocots.ResultsTo characterize BR-related processes in the model grass Brachypodium distachyon, we studied the response of these plants to the specific BR biosynthesis inhibitor, propiconazole (Pcz). We found that treatments with Pcz produced a dwarf phenotype in B. distachyon seedlings, similar to that observed in Pcz-treated Arabidopsis plants and in characterized BR-deficient mutants. Through bioinformatics analysis, we identified a list of putative homologs of genes known to be involved in BR biosynthesis and signaling in Arabidopsis, such as DWF4, BR6OX2, CPD, BRI1, and BIN2. Evaluating the response of these genes to Pcz treatments revealed that candidates for BdDWF4, BR6OX2 and, CPD were under feedback regulation. In addition, Arabidopsis plants heterologously expressing BdDWF4 displayed tall statures and elongated petioles, as would be expected in plants with elevated levels of BRs. Moreover, heterologous expression of BdBIN2 in Arabidopsis resulted in dwarfism, suggesting that BdBIN2 functions as a negative regulator of BR signaling. However, the dwarf phenotypes of Arabidopsis bri1-5, a weak BRI1 mutant allele, were not complemented by overexpression of BdBRI1, indicating that BdBRI1 and BRI1 are not functionally equivalent.ConclusionWe identified components of the BR biosynthetic and signaling pathways in Brachypodium, and provided examples of both similarities and differences in the BR biology of these two plants. Our results suggest a framework for understanding BR biology in monocot crop plants such as Zea mays (maize) and Oryza sativa (rice).Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0965-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Identification of brassinosteroid genes in Brachypodium distachyon

Corvalán

Choe

2017

BMC Plant Biol

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“…The transcription factor LATERAL ORGAN BOUNDARIES (LOB) in Arabidopsis mediates this growth restriction by negatively regulating brassinosteroid (BR) signaling. BR promotes organ growth in various developmental contexts [43]. lob mutants have organ separation defects, namely in junctions between the main stem, axillary stem, and cauline leaves.…”

Section: Boundariesmentioning

confidence: 99%

Organogenesis in plants: initiation and elaboration of leaves

Sluis

Hake

2015

Trends in Genetics

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“…Their many roles throughout the plant life cycle are enabled by a central signaling module, which controls the expression of thousands of target genes (Sun et al, 2010;Yu et al, 2011). This module has been heavily studied in the last two decades, thereby considerably improving our understanding, which has been recently summarized in a number of excellent reviews (Guo et al, 2013;Wang et al, 2014;Singh and Savaldi-Goldstein, 2015). Here, we summarize our integrated model of BR-GA crosstalk (Unterholzner et al, 2015) and explain why we consider BRs as "master regulators" of GA biosynthesis.…”

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confidence: 99%