Growth-disrupting Murraya koenigii leaf extracts on Anopheles gambiae larvae and identification of associated candidate bioactive constituents

Mang’era, Clarence M.; Hassanali, Ahmed; Khamis, Fathiya M.; Rono, Martin K.; Lwande, Wilber; Mbogo, Charles M.; Mireji, Paul O.

doi:10.1016/j.actatropica.2018.12.009

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…For instance, the limonoid azadirachtin isolated from Azadirachta indica (Meliaceae) is a well‐known example of a plant derived insecticide: further, numerous studies have reported larvicidal effect of plant extracts 9 or their constituents on diverse mosquito species 10,11 . Most of these larvicidal activities are attributed to specific classes of phytochemicals such as anthraquinones, 11 alkaloids, 12–14 limonoids 9,15,16 and terpenoids 17,18 …”

Section: Introductionmentioning

confidence: 99%

Derivatization increases mosquito larvicidal activity of the sesquiterpene lactone parthenin isolated from the invasive weed Parthenium hysterophorus

Milugo

Tchouassi

Kavishe

et al. 2020

Pest Management Science

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BACKGROUND Extracts of the invasive weed Parthenium hysterophorus (Asteraceae) have been shown to possess larvicidal activity against a wide range of disease vectors. However, the phytochemicals responsible for the larvicidal activity from this plant remain unidentified. Here, we isolated the major sesquiterpene lactone, parthenin (1) from the plant and synthesized two derivatives [ethylene glycol (2) and azide (3) derivatives] targeting the α,β‐unsaturated carbonyl group, previously known to account for its biological activity such as toxicity towards cells and microorganism. All three compounds were screened for larvicidal activity against the African malaria vector Anopheles gambiae. RESULTS The larval mortality of ethylene glycol derivative (2) and 2α‐azidocoronopilin (3) were approximately two–four‐fold higher than that of parthenin (1) and neem oil with LC50 values of 37 and 66 mg L−1, respectively. Parthenin (1) and the positive control, neem oil, had comparable median lethal concentration (LC50) values of 154 and 121 mg L−1, respectively. In assays with binary combinations of the three compounds, larvicidal activity followed the order: parthenin (1) + 2α‐azidocoronopilin (3) (LC50 = 14 mg L−1) > parthenin (1) + ethylene glycol derivative (2) (LC50 = 109 mg L−1), > blend of 2α‐azidocoronopilin (3) and ethylene glycol derivative (2) (LC50 = 200 mg L−1). CONCLUSION Structural modification of parthenin (1) through addition of hydroxyl groups increases its larvicidal effects. These findings advance the use of structural modification approach in the development of lead chemical molecules for potential exploitation in larval source management.

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Section: Introductionmentioning

confidence: 99%

Derivatization increases mosquito larvicidal activity of the sesquiterpene lactone parthenin isolated from the invasive weed Parthenium hysterophorus

Milugo

Tchouassi

Kavishe

et al. 2020

Pest Management Science

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Bioactivity of Couroupita guianensis Aubl. against filarial and dengue vectors and non-target fish

Maheswaran

Baskar²,

Ignacimuthu³

et al. 2019

South African Journal of Botany

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Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals

et al. 2021

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Background Insect growth regulators (IGRs) can control insect vector populations by disrupting growth and development in juvenile stages of the vectors. We previously identified and described the curry tree (Murraya koenigii (L.) Spreng) phytochemical leaf extract composition (neplanocin A, 3-(1-naphthyl)-l-alanine, lumiflavine, terezine C, agelaspongin and murrayazolinol), which disrupted growth and development in Anopheles gambiae sensu stricto mosquito larvae by inducing morphogenetic abnormalities, reducing locomotion and delaying pupation in the mosquito. Here, we attempted to establish the transcriptional process in the larvae that underpins these phenotypes in the mosquito. Methods We first exposed third-fourth instar larvae of the mosquito to the leaf extract and consequently the inherent phytochemicals (and corresponding non-exposed controls) in two independent biological replicates. We collected the larvae for our experiments sampled 24 h before peak pupation, which was 7 and 18 days post-exposure for controls and exposed larvae, respectively. The differences in duration to peak pupation were due to extract-induced growth delay in the larvae. The two study groups (exposed vs control) were consequently not age-matched. We then sequentially (i) isolated RNA (whole larvae) from each replicate treatment, (ii) sequenced the RNA on Illumina HiSeq platform, (iii) performed differential bioinformatics analyses between libraries (exposed vs control) and (iv) independently validated the transcriptome expression profiles through RT-qPCR. Results Our analyses revealed significant induction of transcripts predominantly associated with hard cuticular proteins, juvenile hormone esterases, immunity and detoxification in the larvae samples exposed to the extract relative to the non-exposed control samples. Our analysis also revealed alteration of pathways functionally associated with putrescine metabolism and structural constituents of the cuticle in the extract-exposed larvae relative to the non-exposed control, putatively linked to the exoskeleton and immune response in the larvae. The extract-exposed larvae also appeared to have suppressed pathways functionally associated with molting, cell division and growth in the larvae. However, given the age mismatch between the extract-exposed and non-exposed larvae, we can attribute the modulation of innate immune, detoxification, cuticular and associated transcripts and pathways we observed to effects of age differences among the larvae samples (exposed vs control) and to exposures of the larvae to the extract. Conclusions The exposure treatment appears to disrupt cuticular development, immune response and oxidative stress pathways in Anopheles gambiae s.s larvae. These pathways can potentially be targeted in development of more efficacious curry tree phytochemical-based IGRs against An. gambiae s.s mosquito larvae.

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Growth-disrupting Murraya koenigii leaf extracts on Anopheles gambiae larvae and identification of associated candidate bioactive constituents

Cited by 3 publications

References 55 publications

Derivatization increases mosquito larvicidal activity of the sesquiterpene lactone parthenin isolated from the invasive weed Parthenium hysterophorus

Derivatization increases mosquito larvicidal activity of the sesquiterpene lactone parthenin isolated from the invasive weed Parthenium hysterophorus

Bioactivity of Couroupita guianensis Aubl. against filarial and dengue vectors and non-target fish

Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals

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