Growth dynamics of major microbial populations during biodegradation of o-phthalate in anaerobic sediment slurries under a CO2/H2 atmosphere

Liu, S.-M.; Lin, Yang-Ding; Tsai, Tung-Tso

doi:10.1016/j.chemosphere.2004.09.074

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…Until recently, knowledge of anaerobic degradation of PA in obligately anaerobic bacteria was restricted to a few studies with sediment slurries and enrichment cultures (Kleerebezem et al ., ; Kleerebezem et al ., ; Liu and Chi, ; Liu et al ., ; Liu et al ., ). Further, a Pelotomaculum isopthalicum species has been described that grows with all three phthalate isomers in syntrophic association with a methanogen (Qiu et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Microbial degradation of phthalates: biochemistry and environmental implications

Boll

Geiger

Junghare

et al. 2019

Environ Microbiol Rep

133

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Summary The environmentally relevant xenobiotic esters of phthalic acid (PA), isophthalic acid (IPA) and terephthalic acid (TPA) are produced on a million ton scale annually and are predominantly used as plastic polymers or plasticizers. Degradation by microorganisms is considered as the most effective means of their elimination from the environment and proceeds via hydrolysis to the corresponding PA isomers and alcohols under oxic and anoxic conditions. Further degradation of PA, IPA and TPA differs fundamentally between anaerobic and aerobic microorganisms. The latter introduce hydroxyl functionalities by dioxygenases to facilitate subsequent decarboxylation by either aromatizing dehydrogenases or cofactor‐free decarboxylases. In contrast, anaerobic bacteria activate the PA isomers to the respective thioesters using CoA ligases or CoA transferases followed by decarboxylation to the central intermediate benzoyl‐CoA. Decarboxylases acting on the three PA CoA thioesters belong to the UbiD enzyme family that harbour a prenylated flavin mononucleotide (FMN) cofactor to achieve the mechanistically challenging decarboxylation. Capture of the extremely instable PA‐CoA intermediate is accomplished by a massive overproduction of phthaloyl‐CoA decarboxylase and a balanced production of PA‐CoA forming/decarboxylating enzymes. The strategy of anaerobic phthalate degradation probably represents a snapshot of an ongoing evolution of a xenobiotic degradation pathway via a short‐lived reaction intermediate.

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Section: Introductionmentioning

confidence: 97%

Microbial degradation of phthalates: biochemistry and environmental implications

Boll

Geiger

Junghare

et al. 2019

Environ Microbiol Rep

133

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show abstract

“…Only very little is known about anaerobic phthalate degradation in strictly anaerobic bacteria. Phthalate degradation coupled to sulphate reduction and methanogenesis was observed in sediment slurries or enrichment cultures (Kleerebezem et al ., ; Liu and Chi, ; Chang et al ., ; Liu et al ., ; Liu et al ., ), and Pelotomaculum species were reported to degrade phthalate in syntrophic association with hydrogenotrophic methanogens (Qiu et al ., ). However, the genes and enzymes involved in phthalate degradation have not yet been studied in an obligately anaerobic organism.…”

Section: Introductionmentioning

confidence: 99%

Enzymes involved in phthalate degradation in sulphate‐reducing bacteria

Geiger

Junghare

Mergelsberg

et al. 2019

Environmental Microbiology

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Summary The complete degradation of the xenobiotic and environmentally harmful phthalate esters is initiated by hydrolysis to alcohols and o‐phthalate (phthalate) by esterases. While further catabolism of phthalate has been studied in aerobic and denitrifying microorganisms, the degradation in obligately anaerobic bacteria has remained obscure. Here, we demonstrate a previously overseen growth of the δ‐proteobacterium Desulfosarcina cetonica with phthalate/sulphate as only carbon and energy sources. Differential proteome and CoA ester pool analyses together with in vitro enzyme assays identified the genes, enzymes and metabolites involved in phthalate uptake and degradation in D. cetonica. Phthalate is initially activated to the short‐lived phthaloyl‐CoA by an ATP‐dependent phthalate CoA ligase (PCL) followed by decarboxylation to the central intermediate benzoyl‐CoA by an UbiD‐like phthaloyl‐CoA decarboxylase (PCD) containing a prenylated flavin cofactor. Genome/metagenome analyses predicted phthalate degradation capacity also in the sulphate‐reducing Desulfobacula toluolica, strain NaphS2, and other δ‐proteobacteria. Our results suggest that phthalate degradation proceeds in all anaerobic bacteria via the labile phthaloyl‐CoA that is captured and decarboxylated by highly abundant PCDs. In contrast, two alternative strategies have been established for the formation of phthaloyl‐CoA, the possibly most unstable CoA ester in biology.

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Influences of organic loading disturbances on the performance of anaerobic filter process to treat purified terephthalic acid wastewater

Joung

Lee

Choi

et al. 2009

Bioresource Technology

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Growth dynamics of major microbial populations during biodegradation of o-phthalate in anaerobic sediment slurries under a CO2/H2 atmosphere

Cited by 9 publications

References 24 publications

Microbial degradation of phthalates: biochemistry and environmental implications

Microbial degradation of phthalates: biochemistry and environmental implications

Enzymes involved in phthalate degradation in sulphate‐reducing bacteria

Influences of organic loading disturbances on the performance of anaerobic filter process to treat purified terephthalic acid wastewater

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