Growth enhancement and drought tolerance of hybrid poplar upon inoculation with endophyte consortia

Khan, Zareen; Rho, Hyun Soo; Firrincieli, Andrea; Hung, Shang Han; Luna, Virginia; Masciarelli, Oscar; Kim, Sung Hyun; Doty, Sharon L.

doi:10.1016/j.cpb.2016.08.001

Cited by 153 publications

(92 citation statements)

References 86 publications

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“…Thus, fungal endophytes increase osmolytes like TSS and proline, which improve the membrane stability under drought condition, that in turn, is evidenced by low level of peroxidation of cell membranes. The presence of flavonoids and phenolic compounds has been noted in plant metabolomic profiles driven by endophytes when subjected to even low levels of abiotic stress, which eliminate hydroxyl groups, reducing the disintegration of hydroperoxides and inactivating lipid free radicals (Khan et al, 2016). The latter suggests that the presence of fungal endophytes contributes to improving the antioxidant potential in plants (Hamilton et al, 2012), and that the presence of endophytes is more relevant in activating this protective mechanism than the level of stress experienced by the plant.…”

Section: Discussionmentioning

confidence: 99%

Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica

et al. 2020

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Functional symbiosis is considered one of the successful mechanisms by which plants that inhabit extreme environment improve their ability to tolerate different types of stress. One of the most conspicuous type of symbiosis is the endophyticism. This interaction has been noted to play a role in the adaptation of the native vascular plant Colobanthus quitensis to the stressful environments of Antarctica, characterized by low temperatures and extreme aridity. Projections of climate change for this ecosystem indicate that abiotic conditions will be less limiting due to an increase in temperature and water availability in the soil. Due to this decrease in stress induced by the climate change, it has been suggested that the positive role of fungal endophytes on performance of C. quitensis plants would decrease. In this study, we evaluated the role of endophytic fungi on osmoprotective molecules (sugar production, proline, oxidative stress) and gene expression (CqNCED1, CqABCG25, and CqRD22) as well as physiological traits (stomatal opening, net photosynthesis, and stomatal conductance) in individuals of C. quitensis. Individual plants of C. quitensis with (E+) and without (E−) endophytic fungi were exposed to simulated conditions of increased water availability (W+), having the current limiting water condition (W−) in Antarctica as control. The results reveal an endophyte-mediated lower oxidative stress, higher production of sugars and proline in plants. In addition, E+ plants showed differential expressions in genes related with drought stress response, which was more evident in W− than in W+. These parameters corresponded with increased physiological mechanisms such as higher net photosynthesis, stomatal opening and conductance under presence of endophytes (E+) as well as the projected water condition (W+) for Antarctica. These results suggest that the presence of fungal endophytes plays a positive role in favoring

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Section: Discussionmentioning

confidence: 99%

Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica

et al. 2020

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“…Environmental variables such as radiation, precipitation, temperature and humidity have a direct effect on stomata opening and, therefore, play a pivotal role in regulating CO 2 uptake for photosynthesis. In this respect, several studies have confirmed that endophytes can affect host fitness under drought conditions having a direct effect on stomata conductivity (Elmi and West, 1995;Arnold and Engelbrecht, 2007;Khan et al, 2016;Rho et al, 2018). While these studies have been conducted under controlled conditions, the importance of the phyllosphere microbial communities in natural ecosystems is still poorly understood (Laforest-Lapointe et al, 2016, 2017.…”

Section: Discussionmentioning

confidence: 99%

“…Poplar (Populus) and willow (Salix) trees of the Salicaceae have a wide global distribution, both in native riparian forests across the Northern Hemisphere and in planted forests, accounting for more than 95 million hectares globally (fao.org). Native poplar trees have a diverse microbiota, many with the ability to fix dinitrogen gas, solubilize phosphate, and promote plant growth and health especially under abiotic stresses such as drought and nutrient limitation (Doty et al, 2005Xin et al, 2009;Khan et al, 2012Khan et al, , 2015Khan et al, , 2016Kandel et al, 2015Kandel et al, , 2017Doty, 2016;Aghai et al, 2019). Beneficial microbiota have been isolated from hybrid poplar trees grown in contaminated sites, in field sites, or in tissue culture (Moore et al, 2006;Ulrich et al, 2008;Barac et al, 2009;Scherling et al, 2009;Taghavi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Influences of Climate on Phyllosphere Endophytic Bacterial Communities of Wild Poplar

et al. 2020

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Plant-associated microbial communities play a central role in the plant response to biotic and abiotic stimuli, improving plant fitness under challenging growing conditions. Many studies have focused on the characterization of changes in abundance and composition of root-associated microbial communities as a consequence of the plant response to abiotic factors such as altered soil nutrients and drought. However, changes in composition in response to abiotic factors are still poorly understood concerning the endophytic community associated to the phyllosphere, the above-ground plant tissues. In the present study, we applied high-throughput 16S rDNA gene sequencing of the phyllosphere endophytic bacterial communities colonizing wild Populus trichocarpa (black cottonwood) plants growing in native, nutrient-limited environments characterized by hot-dry (xeric) riparian zones (Yakima River, WA), riparian zones with mid hot-dry (Tieton and Teanaway Rivers, WA) and moist (mesic) climates (Snoqualmie, Skykomish and Skagit Rivers, WA). From sequencing data, 587 Amplicon Sequence Variants (ASV) were identified. Surprisingly, our data show that a core microbiome could be found in phyllosphere-associated endophytic communities in trees growing on opposite sides of the Cascades Mountain Range. Considering only taxa appearing in at least 90% of all samples within each climatic zone, the core microbiome was dominated only by two ASVs affiliated Pseudomonadaceae and two ASVs of the Enterobacteriaceae family. Alpha-diversity measures indicated that plants colonizing hot-dry environments showed a lower diversity than those from mid hot-dry and moist climates. Beta-diversity measures showed that bacterial composition was significantly different across sampling sites. Accordingly, we found that specific ASV affiliated to Pseudomonadaceae and Enterobacteriaceae were significantly more abundant in the phyllosphere endophytic community colonizing plants adapted to the xeric environment. In summary, this study highlights that sampling site is the major driver of variation and that only a few ASV showed a distribution that significantly correlated to climate variables.

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“…The propagation protocol for seedling production is described elsewhere [42]. Seven bacterial strains and one yeast strain (Table 1) were chosen based on strong growth in nitrogen-free media, the presence of the nitrogen fixation gene (nifH) [20,21], production of phytohormones [43] and/or their robust plant growth-promoting abilities. …”

Section: Plant Materials and Endophytesmentioning

confidence: 99%

Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia

Khan

Kandel

Ramos

et al. 2015

Forests

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Douglas-fir (Pseudotsuga menziesii) seedlings are periodically challenged by biotic and abiotic stresses. The ability of endophytes to colonize the interior of plants could confer benefits to host plants that may play an important role in plant adaptation to environmental changes. In this greenhouse study, nursery-grown Douglas-fir seedlings were inoculated with diazotrophic endophytes previously isolated from poplar and willow trees and grown for fifteen months in nutrient-poor conditions. Inoculated seedlings had significant increases in biomass (48%), root length (13%) and shoot height (16%) compared to the control seedlings. Characterization of these endophytes for symbiotic traits in addition to nitrogen fixation revealed that they can also solubilize phosphate and produce siderophores. Colonization was observed through fluorescent microscopy in seedlings inoculated with gfp-and mkate-tagged strains. Inoculation with beneficial endophytes could prove to be valuable for increasing the production of planting stocks in forest nurseries.

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Growth enhancement and drought tolerance of hybrid poplar upon inoculation with endophyte consortia

Cited by 153 publications

References 86 publications

Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica

Fungal Endophytes Exert Positive Effects on Colobanthus quitensis Under Water Stress but Neutral Under a Projected Climate Change Scenario in Antarctica

Influences of Climate on Phyllosphere Endophytic Bacterial Communities of Wild Poplar

Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia

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