Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia

Khan, Zareen; Kandel, Shyam L.; Ramos, Daniela N.; Ettl, Gregory J.; Kim, Sung Hyun; Doty, Sharon L.

doi:10.3390/f6103582

Cited by 38 publications

(46 citation statements)

References 52 publications

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“…Several poplar and willow endophytes are diazotrophs with the ability of producing phytohormones and siderophores, and solubilizing inorganic phosphates (Khan et al, 2015; Doty et al, 2016). A recent study showed that inoculated Salicaceae endophytes in hybrid poplar plants can contribute about 65% of the total N in the leaves and increase plant biomass through BNF (Knoth et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes

et al. 2017

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Microbial communities in the endosphere of Salicaceae plants, poplar (Populus trichocarpa) and willow (Salix sitchensis), have been demonstrated to be important for plant growth promotion, protection from biotic and abiotic stresses, and degradation of toxic compounds. Our study aimed to investigate bio-control activities of Salicaceae endophytes against various soil borne plant pathogens including Rhizoctonia solani AG-8, Fusarium culmorum, Gaeumannomyces graminis var. tritici, and Pythium ultimum. Additionally, different plant growth promoting traits such as biological nitrogen fixation (BNF), indole-3-acetic acid (IAA) biosynthesis, phosphate solubilization, and siderophore production were assessed in all bio-control positive strains. Burkholderia, Rahnella, Pseudomonas, and Curtobacterium were major endophyte genera that showed bio-control activities in the in-vitro assays. The bio-control activities of Burkholderia strains were stronger across all tested plant pathogens as compared to other stains. Genomes of sequenced Burkholderia strains WP40 and WP42 were surveyed to identify the putative genes involved in the bio-control activities. The ocf and hcnABC gene clusters responsible for biosynthesis of the anti-fungal metabolites, occidiofungin and hydrogen cyanide, are present in the genomes of WP40 and WP42. Nearly all endophyte strains showing the bio-control activities produced IAA, solubilized tricalcium phosphate, and synthesized siderophores in the culture medium. Moreover, some strains reduced acetylene into ethylene in the acetylene reduction assay, a common assay used for BNF. Salicaceae endophytes could be useful for bio-control of various plant pathogens, and plant growth promotion possibly through the mechanisms of BNF, IAA production, and nutrient acquisition.

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Section: Introductionmentioning

confidence: 99%

An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes

et al. 2017

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“…These endophytes either as single strain or multi-strain inoculum have previously exhibited mutualistic behavior when added to other plant species including grasses, corn, rice, Douglas-fir and a variety of crop plants (Xin et al, 2009;Knoth et al, 2013;Knoth et al, 2014;Khan et al, 2012;Khan et al, 2015;DaSilveria et al, 2016). Combined application of endophytes can result in larger effects than those possible with individual inoculations (Knoth et al, 2013;Knoth et al, 2014;Rogers et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Consortium Application of Endophytic Bacteria and Fungi Improves Grain Yield and Physiological Attributes in Advanced Lines of Bread Wheat

Muhae-Ud-Din¹,

Ali²,

Naveed³

et al. 2018

Turkish JAF Sci.Tech.

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Increasing human population places pressure on agriculture. To feed this population, two time increase in the current wheat production is needed. Today agriculture is becoming input intensive with more reliance on synthetic fertilizers and agrochemicals to fulfil the feed demand of the growing numbers. Use of synthetic fertilizer since last few years is impacting the soil quality. In this scenario, the use of beneficial endophytic microbes is an attractive strategy to overcome the use of synthetic products. To investigate the effect of consortium application of endophytic bacteria and fungus on plant growth, grain yield moisture status, a pot experiment was conducted in different wheat lines. It comprised four treatments like control, application of bacterial strain Bacillus sp. MN54, fungal strain Trichoderma sp. MN6, and their consortium (Bacillus sp. MN54 + Trichoderma sp. MN6). The effect of consortium application was more prominent and significantly different from the sole application of bacteria and fungus. The results showed that with a consortium application of endophytic bacteria and fungus, there was 28.6, 4.3, -6.3 and -3.7% increases in flag leaf area, chlorophyll content, relative membrane permeability and water content respectively. Consortia of endophytic microbes also resulted in the yield enhancement through the betterment of various yield attributes like number of spikelet's, grains per spike and grain yield per plant (32.2, 25.8 and 30.8%, respectively). So, consortia of endophytic microbes can greatly promote the progress of plants in dry land agriculture and increase the yield in an environmentally sustainable way.

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“…Poplar (Populus) and willow (Salix) trees of the Salicaceae have a wide global distribution, both in native riparian forests across the Northern Hemisphere and in planted forests, accounting for more than 95 million hectares globally (fao.org). Native poplar trees have a diverse microbiota, many with the ability to fix dinitrogen gas, solubilize phosphate, and promote plant growth and health especially under abiotic stresses such as drought and nutrient limitation (Doty et al, 2005Xin et al, 2009;Khan et al, 2012Khan et al, , 2015Khan et al, , 2016Kandel et al, 2015Kandel et al, , 2017Doty, 2016;Aghai et al, 2019). Beneficial microbiota have been isolated from hybrid poplar trees grown in contaminated sites, in field sites, or in tissue culture (Moore et al, 2006;Ulrich et al, 2008;Barac et al, 2009;Scherling et al, 2009;Taghavi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Influences of Climate on Phyllosphere Endophytic Bacterial Communities of Wild Poplar

et al. 2020

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Plant-associated microbial communities play a central role in the plant response to biotic and abiotic stimuli, improving plant fitness under challenging growing conditions. Many studies have focused on the characterization of changes in abundance and composition of root-associated microbial communities as a consequence of the plant response to abiotic factors such as altered soil nutrients and drought. However, changes in composition in response to abiotic factors are still poorly understood concerning the endophytic community associated to the phyllosphere, the above-ground plant tissues. In the present study, we applied high-throughput 16S rDNA gene sequencing of the phyllosphere endophytic bacterial communities colonizing wild Populus trichocarpa (black cottonwood) plants growing in native, nutrient-limited environments characterized by hot-dry (xeric) riparian zones (Yakima River, WA), riparian zones with mid hot-dry (Tieton and Teanaway Rivers, WA) and moist (mesic) climates (Snoqualmie, Skykomish and Skagit Rivers, WA). From sequencing data, 587 Amplicon Sequence Variants (ASV) were identified. Surprisingly, our data show that a core microbiome could be found in phyllosphere-associated endophytic communities in trees growing on opposite sides of the Cascades Mountain Range. Considering only taxa appearing in at least 90% of all samples within each climatic zone, the core microbiome was dominated only by two ASVs affiliated Pseudomonadaceae and two ASVs of the Enterobacteriaceae family. Alpha-diversity measures indicated that plants colonizing hot-dry environments showed a lower diversity than those from mid hot-dry and moist climates. Beta-diversity measures showed that bacterial composition was significantly different across sampling sites. Accordingly, we found that specific ASV affiliated to Pseudomonadaceae and Enterobacteriaceae were significantly more abundant in the phyllosphere endophytic community colonizing plants adapted to the xeric environment. In summary, this study highlights that sampling site is the major driver of variation and that only a few ASV showed a distribution that significantly correlated to climate variables.

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Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia

Cited by 38 publications

References 52 publications

An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes

An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes

Consortium Application of Endophytic Bacteria and Fungi Improves Grain Yield and Physiological Attributes in Advanced Lines of Bread Wheat

Influences of Climate on Phyllosphere Endophytic Bacterial Communities of Wild Poplar

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