Growth enhancement of bioethanol-producing microbe Clostridium autoethanogenum by changing culture medium composition

Park, Soeun; Ahn, Bohye; Kim, Young-Kee

doi:10.1016/j.biteb.2019.03.012

Cited by 15 publications

(8 citation statements)

References 23 publications

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“…Growth of C. ljungdahlii on CO • CO 2 4:1 using the "Tanner_mod" medium did not show the lag phase, compared to other conditions, probably due to the presence of the optimal YE concentration [41,53]. The "Tan_Val" medium afforded a maximum OD 600nm (1.56 ± 0.04 at day 6) similar to the one reached using the "Tanner_mod" medium (1.47 ± 0.02 at day 5) and led to the highest specific growth rate (0.0346 ± 0.0007 gCDW/L/h, Table S5), whereas growth on the "Valgepea_mod" and on the "Tan_Val + Fe" media, led to lower maximum OD 600nm values (1.10 ± 0.13 at day 7 and 1.33 ± 0.04 at day 10, respectively), as shown in Figure 5A.…”

Section: Influence Of Cultivation Medium On 23-bdo Production From Co • Co 2 Using Clostridium Ljungdahliimentioning

confidence: 89%

“…Moreover, the concentration of most constituents of the vitamin solution of the "Valgepea_mod" medium was increased by 10-fold, with the exception of vitamin B12, which was barely detectable in the "Tanner_mod" medium and was augmented by around 500-fold in the "Valgepea_mod" medium (Table 2). Although some studies did not provide evidence that manipulating vitamin concentrations could affect either the growth or the production profile of C. autoethanogenum [40,53], it was shown that increasing the concentration of specific vitamins, such as pantothenic acid and biotin, above the cellular requirements of an acetogen grown on a CO-rich gas mixture led to an increase in 2,3-BDO production [56]. Furthermore, a role in anaerobic CO 2 fixation was described for vitamin B12, a cofactor for methyl group transfer reactions in the Wood-Ljungdahl pathway [57], whose concentration was substantially increased in the "Valgepea_mod" medium.…”

Section: Influence Of Cultivation Medium On 23-bdo Production From Co • Co 2 Using Clostridium Ljungdahliimentioning

confidence: 99%

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Screening of Gas Substrate and Medium Effects on 2,3-Butanediol Production with C. ljungdahlii and C. autoethanogenum Aided by Improved Autotrophic Cultivation Technique

et al. 2021

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Gas fermentation by acetogens of the genus Clostridium is an attractive technology since it affords the production of biochemicals and biofuels from industrial waste gases while contributing to mitigate the carbon cycle alterations. The acetogenic model organisms C. ljungdahlii and C. autoethanogenum have already been used in large scale industrial fermentations. Among the natural products, ethanol production has already attained industrial scale. However, some acetogens are also natural producers of 2,3-butanediol (2,3-BDO), a platform chemical of relevant industrial interest. Here, we have developed a lab-scale screening campaign with the aim of enhancing 2,3-BDO production. Our study generated comparable data on growth and 2,3-BDO production of several batch gas fermentations using C. ljungdahlii and C. autoethanogenum grown on different gas substrates of primary applicative interest (CO2 · H2, CO · CO2, syngas) and on different media featuring different compositions as regards trace metals, mineral elements and vitamins. CO · CO2 fermentation was found to be preferable for the production of 2,3-BDO, and a fair comparison of the strains cultivated in comparable conditions revealed that C. ljungdahlii produced 3.43-fold higher titer of 2,3-BDO compared to C. autoethanogenum. Screening of different medium compositions revealed that mineral elements, Zinc and Iron exert a major positive influence on 2,3-BDO titer and productivity. Moreover, the CO2 influence on CO fermentation was explored by characterizing C. ljungdahlii response with respect to different gas ratios in the CO · CO2 gas mixtures. The screening strategies undertaken in this study led to the production of 2.03 ± 0.05 g/L of 2,3-BDO, which is unprecedented in serum bottle experiments.

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Section: Influence Of Cultivation Medium On 23-bdo Production From Co • Co 2 Using Clostridium Ljungdahliimentioning

confidence: 89%

Section: Influence Of Cultivation Medium On 23-bdo Production From Co • Co 2 Using Clostridium Ljungdahliimentioning

confidence: 99%

Screening of Gas Substrate and Medium Effects on 2,3-Butanediol Production with C. ljungdahlii and C. autoethanogenum Aided by Improved Autotrophic Cultivation Technique

et al. 2021

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“…The experimental design was recommended by the statistical software Design Expert 7.0 based on the minimum and maximum values of the trace elements to be optimized. The concentration ranges for the trace elements were determined based on their amounts reported in the literature [19][20][21][22][23] . The lowest values were set to 0 g/L.…”

Section: Trace Elements Plackett-burman Methods and Bottle Batch Experimentsmentioning

confidence: 99%

“…The influence of trace metals on the Wood-Ljungdahl pathway is a critical topic that has received much attention in recent years [21]. Apart from the kind of trace element used, the concentrations of the trace element have an effect on the syngas fermentation process.…”

Section: Optimized Trace Metal Composition For Ethanolmentioning

confidence: 99%

Design of Low-Cost Ethanol Production Medium from Syngas: An Optimization of Trace Metals for Clostridium ljungdahlii

et al. 2021

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Syngas fermentation via the Wood-Ljungdahl (WL) pathway is a promising approach for converting gaseous pollutants (CO and CO2) into high-value commodities. Because the WL involves several enzymes with trace metal components, it requires an adequate supply of micronutrients in the fermentation medium for targeted bioprocessing such as bioethanol production. Plackett-Burman statistical analysis was performed to examine the most efficient trace elements (Ni, Mg, Ca, Mn, Co, Cu, B, W, Zn, Fe, and Mo) and their concentrations for Clostridium ljungdahlii on ethanol production. Overall, 1.5 to 2.5 fold improvement in ethanol production could be achieved with designed trace element concentrations. The effects of tungsten and copper on ethanol and biomass production were determined to be the most significant, respectively. The model developed was statistically significant and has the potential to significantly decrease the cost of trace element solutions by 18–22%. This research demonstrates the critical importance of optimizing the medium for syngas fermentation in terms of product distribution and economic feasibility.

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“…At the same time, understanding of the gas fermentation bioprocess has also improved with the characterisation of the effects of gas-liquid mass transfer, feed gas and media composition, pH shifts, and mixed cultures on the biocatalyst and fermentation performance, e.g. gas uptake, biomass level, product profile, yields, and rates (Cotter et al, 2009;Abubackar et al, 2012;Abubackar et al, 2015;Valgepea et al, 2017bValgepea et al, , 2018Park et al, 2019;Heffernan et al, 2020;Esquivel-Elizondo et al, 2017;Diender et al, 2019). However, the effects of the specific growth rate (μ) of the cells on acetogen metabolism and on the gas fermentation bioprocess have not yet been established.…”

Section: Introductionmentioning

confidence: 99%

Faster growth enhances low carbon fuel and chemical production through gas fermentation

Lima

Ingelman

Brahmbhatt

et al. 2022

Preprint

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Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespread deployment of the commercialised technology. Acetogens are particularly attractive biocatalysts but effects of the key physiological parameter – specific growth rate (μ) – on acetogen metabolism and the gas fermentation bioprocess have not been established yet. Here, we investigate the (μ-dependent bioprocess performance of the model-acetogen Clostridium autoethanogenum in CO and syngas (CO+CO2+H2) grown chemostat cultures and assess systems-level metabolic responses using gas analysis, metabolomics, transcriptomics, and metabolic modelling. We were able to obtain steady-states up to μ ~2.8 day-1 (~0.12 h-1) and show that faster growth supports both higher yields and productivities for reduced by-products ethanol and 2,3-butanediol. Transcriptomics data revealed differential expression of 1,337 genes with increasing (μ) and suggest that C. autoethanogenum uses transcriptional regulation to a large extent for facilitating faster growth. Metabolic modelling showed significantly increased fluxes for faster growing cells that were, however, not accompanied by gene expression changes in key catabolic pathways for CO and H2 metabolism. Cells thus seem to maintain sufficient 'baseline' gene expression to rapidly respond to CO and H2 availability without delays to kick-start metabolism. Our work advances understanding of transcriptional regulation in acetogens and shows that faster growth of the biocatalyst improves the gas fermentation bioprocess.

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Growth enhancement of bioethanol-producing microbe Clostridium autoethanogenum by changing culture medium composition

Cited by 15 publications

References 23 publications

Screening of Gas Substrate and Medium Effects on 2,3-Butanediol Production with C. ljungdahlii and C. autoethanogenum Aided by Improved Autotrophic Cultivation Technique

Screening of Gas Substrate and Medium Effects on 2,3-Butanediol Production with C. ljungdahlii and C. autoethanogenum Aided by Improved Autotrophic Cultivation Technique

Design of Low-Cost Ethanol Production Medium from Syngas: An Optimization of Trace Metals for Clostridium ljungdahlii

Faster growth enhances low carbon fuel and chemical production through gas fermentation

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