This
study investigated the toxicity of trichlorfon (TCF) to the
freshwater algae Chlamydomonas reinhardtii, as well
as its biodegradation and metabolic fate. The growth of C.
reinhardtii decreased with increasing TCF concentration,
and the maximum inhibition ratio was 51.3% at 200 mg L–1 TCF compared to the control. Analyses of pigment content, chlorophyll
fluorescence, and antioxidant enzymes indicated that C. reinhardtii can produce resistance and acclimatize to the presence of TCF. The
variations in pH during cultivation suggested that photosynthetic
microalgae have innate advantages over bacteria and fungi in remediating
TCF. A 100% biodegradation rate was achieved at a maximum concentration
of 100 mg L–1 TCF. Ten metabolites were identified
by GC–MS, and the degradation pathways of TCF by the algae
were proposed. This research demonstrated that C. reinhardtii is highly tolerant to and can efficiently degrade TCF. Thus, C. reinhardtii can be used to remove traces of TCF from
natural water environments and to treat TCF-contaminated wastewater.