Growth factor and cytokine expression of human mesenchymal stromal cells is not altered in an in vitro model of tissue damage

Montzka, Katrin; Führmann, Tobias; Müller‐Ehmsen, Jochen; Wöltje, Michael; Brook, Gary

doi:10.3109/14653249.2010.501789

Cited by 18 publications

(16 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Beneficial effects of (activated) MSC preparations in highly inflammatory conditions have been tracked down to the secretion of one specific protein (TNFα-stimulated gene 6 protein, TSG6) [47] via decreasing TLR2/NF-κB signaling. Consistent with the above, our data show considerable standard deviation in cytokine expression in MSC supernatants, confirming results in human MSCs [48]. Also, the source of the MSCs highly influences the characteristics of the cells and might explain why first tests did not detect differences between MSCs derived from adipose tissue of CKD patients and healthy donors [49].…”

Section: Discussionsupporting

confidence: 88%

Mesenchymal Stem Cells from Rats with Chronic Kidney Disease Exhibit Premature Senescence and Loss of Regenerative Potential

et al. 2014

View full text Add to dashboard Cite

Mesenchymal stem cell (MSC) transplantation has the potential for organ repair. Nevertheless, some factors might lessen the regenerative potential of MSCs, e.g. donor age or systemic disease. It is thus important to carefully assess the patient's suitability for autologous MSC transplantation. Here we investigated the effects of chronic kidney disease (CKD) on MSC function. We isolated bone marrow MSCs from remnant kidney rats (RK) with CKD (CKD-RK-MSC) and found signs of premature senescence: spontaneous adipogenesis, reduced proliferation capacity, active senescence-associated-β-galactosidase, accumulation of actin and a modulated secretion profile. The functionality of CKD-RK-MSCs in vivo was tested in rats with acute anti-Thy1.1-nephritis, where healthy MSCs have been shown to be beneficial. Rats received healthy MSCs, CKD-RK-MSC or medium by injection into the left renal artery. Kidneys receiving healthy MSCs exhibited accelerated healing of glomerular lesions, whereas CKD-RK-MSC or medium exerted no benefit. The negative influence of advanced CKD/uremia on MSCs was confirmed in a second model of CKD, adenine nephropathy (AD). MSCs from rats with adenine nephropathy (CKD-AD-MSC) also exhibited cellular modifications and functional deficits in vivo. We conclude that CKD leads to a sustained loss of in vitro and in vivo functionality in MSCs, possibly due to premature cellular senescence. Considering autologous MSC therapy in human renal disease, studies identifying uremia-associated mechanisms that account for altered MSC function are urgently needed.

show abstract

Section: Discussionsupporting

confidence: 88%

Mesenchymal Stem Cells from Rats with Chronic Kidney Disease Exhibit Premature Senescence and Loss of Regenerative Potential

et al. 2014

View full text Add to dashboard Cite

show abstract

“…42 Once a tissue is damaged, chemokines are secreted and function to recruit cells from the environment to the injury site. The chemotactic response (migration of cells) is mediated by chemokine receptors and their ligands.…”

Section: Discussionmentioning

confidence: 99%

The Implications of the Response of Human Mesenchymal Stromal Cells in Three-Dimensional Culture to Electrical Stimulation for Tissue Regeneration

Hwang

Song

Cho

et al. 2012

Tissue Engineering Part A

View full text Add to dashboard Cite

Earlier, we demonstrated that local electrical stimulation (ES) improved bone and peripheral nerve regeneration. To determine how ES induces the regeneration of different kinds of tissues, we studied the initial ES-induced regeneration process by investigating the expression of chemokines and growth factors from human mesenchymal stromal cells (hMSCs). In particular, we assessed the responses of hMSCs grown in three-dimensional (3D) culture on a collagen sponge, as 3D culture techniques induced cell behavior that was similar to in vivo cell behavior. We also compared the gene expression patterns of monolayer hMSCs with those of 3D hMSCs under the condition that cells in either culture are exposed to the same type of ES. Biphasic pulses did not affect the proliferation of hMSCs in 3D culture significantly at the magnitude applied in previous animal studies showing improved bone and peripheral nerve regeneration. However, ES enhanced the gene expression of growth factors (BMP-2, IGF-1, and VEGF), chemokines (CXCL2, interleukin (IL)-8), and chemokine receptors (CXCR4 and IL-8RB) from hMSCs grown in 3D culture. A particular difference between the 3D and monolayer cultures was found in the expression of chemokine receptors, CXCR4 and IL-8RB, which is related to the homing capabilities of mesenchymal stromal cells. These genes were expressed by cells in 3D cultures, but were not or expressed at extremely low levels by cells grown in monolayer cultures. ES led to a significant increase in the expression of CXCR4 and IL-8RB in both monolayer and 3D hMSCs, but the increase in the monolayer culture was detected at an extremely low level. These results demonstrate that ES increased the expression of a variety of growth factors and chemokine genes from 3D hMSCs, which may explain increased tissue regeneration in vivo, independent of the tissue type. A culture-dependent expression of the CXCR4 gene suggested that cell response to external stimulus in 3D systems may be more accurately reflected in in vivo findings than in monolayer cultures.

show abstract

“…Since the effect of MSC can be detected during the fi rst hours after their administration [11,16], while the number of cells with signs of transdifferentiation de novo is extremely low [16], we can conclude that the key role in the short-term effect of MSC is played by paracrine mechanisms. MSC can produce a variety of growth factors (HGF, VEGF, KGF, and FGF) in great amounts, which probably determine their proliferative and regenerative effects [4,10,[19][20][21]. The antiinfl ammatory effect of MSC transplantation presumably related to elevation of anti-infl ammatory cytokine content (IL-10, endothelial NO synthase, and adiponectin) has been demonstrated not once in in vitro and in vivo experiments [9,14,18,22,25,26].…”

Section: Resultsmentioning

confidence: 93%

Effect of Conditioned Medium and Bone Marrow Stem Cell Lysate on the Course of Acetaminophen-Induced Liver Failure

et al. 2015

View full text Add to dashboard Cite

show abstract

Growth factor and cytokine expression of human mesenchymal stromal cells is not altered in an in vitro model of tissue damage

Cited by 18 publications

References 87 publications

Mesenchymal Stem Cells from Rats with Chronic Kidney Disease Exhibit Premature Senescence and Loss of Regenerative Potential

Mesenchymal Stem Cells from Rats with Chronic Kidney Disease Exhibit Premature Senescence and Loss of Regenerative Potential

The Implications of the Response of Human Mesenchymal Stromal Cells in Three-Dimensional Culture to Electrical Stimulation for Tissue Regeneration

Effect of Conditioned Medium and Bone Marrow Stem Cell Lysate on the Course of Acetaminophen-Induced Liver Failure

Contact Info

Product

Resources

About