Growth factors and canine flexor tendon healing: Initial studies in uninjured and repair models

Duffy, Frederick J.; Seiler, John G.; Gelberman, Richard H.; Hergrueter, Charles A.

doi:10.1016/s0363-5023(05)80284-9

Cited by 115 publications

(41 citation statements)

References 20 publications

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“…Its activity is mediated through its interaction with two related tyrosine kinase receptors, one of which binds all three PDGF chains, and the other binds only one (Ronnstrand et al). Work by Duffy et al (1995) has shown that PDGF is elevated in the healing canine digital flexor tendon, suggesting a role in the healing process. It is thought to play a significant role in the early stages of healing, at which time it induces the synthesis of other growth factors, such as IGF-I (Lynch et al, 1989).…”

Section: Pdgfmentioning

confidence: 99%

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Tendon Healing with Growth Factors

Müller¹,

Todorov²,

Heisterbach³

et al. 2012

Achilles Tendon

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Section: Pdgfmentioning

confidence: 99%

“…In healthy canine flexor tendons an increased expression of bFGF was measured whereas the amount of PDGF was increased in healing tendons (Duffy et al, 1995). Under the influence of PDGF an increased chemotaxis of fibroblasts, proliferation rate and collagen synthesis was observed (Grotendorst, 1988;Pierce et al, 1995).…”

Section: Cytokines Modulationmentioning

confidence: 99%

Tendon Healing with Growth Factors

Müller¹,

Todorov²,

Heisterbach³

et al. 2012

Achilles Tendon

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“…Certain cytokines have been shown to promote tendon healing. [9][10][11] Bone morphogenetic proteins (BMPs) are a family of highly related molecules that form a subgroup under the transforming growth factor-b superfamily. 12 BMP-12, a human homolog of murine growth and differentiation factor-7 (GDF-7), induces the formation of tendon-and ligament-like tissue and is thus of considerable interest in the context of enhancing tendon repair.…”

Section: Introductionmentioning

confidence: 99%

Ex vivo adenoviral transfer of bone morphogenetic protein 12 (BMP-12) cDNA improves Achilles tendon healing in a rat model

et al. 2008

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The aim of our study was to evaluate the histological and biomechanical effects of BMP-12 gene transfer on the healing of rat Achilles tendons using a new approach employing a genetically modified muscle flap. Biopsies of autologous skeletal muscle were transduced with a type-five, first-generation adenovirus carrying the human BMP-12 cDNA (Ad.BMP-12) and surgically implanted around experimentally transected Achilles tendons in a rat model. The effect of gene transfer on healing was evaluated by mechanical and histological testing after 1, 2, 4 and 8 weeks. One week after surgery, the maximum failure load of the healing tendons was significantly increased in the BMP-12 group, compared with the controls, and the tendon stiffness was significantly higher at 1, 2 and 4 weeks. Moreover, the size of the rupture callus was increased in the presence of BMP-12 and there was evidence of accelerated remodeling of the lesion in response to BMP-12. Histological examination showed a much more organized and homogeneous pattern of collagen fibers at all time points in lesions treated with the BMP-12 cDNA muscle graft. Both single fibrils and the collagen fibers had a greater diameter, with a higher degree of collagen crimp than the collagen of the control groups. This was confirmed by sirius red staining in conjunction with polarized light microscopy, which showed a higher shift of small yellow-green fibers to strong yelloworange fibers after 2, 4 and 8 weeks in the presence of BMP-12 cDNA. There was also an earlier shift from fibroblasts to fibrocytes within the healing tendon, with less fat cells present in the tendons of the BMP-12 group compared with the controls. Treatment with BMP-12 cDNA-transduced muscle grafts thus produced a promising acceleration and improvement of tendon healing, particularly influencing early tissue regeneration, leading to quicker recovery and improved biomechanical properties of the Achilles tendon. Further development of this approach could have clinical applications.

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“…[5][6][7][8] Approaches to promote tendon and ligament repair by adding exogenous growth factors have been validated in various animal models. 1,[9][10][11][12] Recent in vitro studies using gene therapy approaches also hold promise for the treatment of tendon injury.…”

Section: Introductionmentioning

confidence: 99%

Effect of osteogenic protein‐1 on the matrix metabolism of bovine tendon cells

Yamada

Akeda

Asanuma

et al. 2007

Journal Orthopaedic Research

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Tendon rupture is a common sports injury in adults. However, the mechanical properties of repair tissue are inferior to those of normal tissue. To accelerate tendon healing, an in vivo approach using growth factors has been applied and has shown evidence for the efficacy of biological stimulation of the repair process. Recombinant human osteogenic protein-1 (rhOP-1) has been shown to be effective in stimulating matrix production by various connective tissues. To test the effect of rhOP-1 on the matrix metabolism of tendon cells in vitro, bovine tendon cells were cultured in monolayer with various doses of rhOP-1 for 7 days. The addition of rhOP-1 to cell culture media resulted in significant increases in cell proliferation, DNA content, and the synthesis of proteoglycans (PGs) and collagen, compared to control cultures. The relative percentage of large PGs in the OP-1 culture was higher than that in the control culture. In conclusion, we show for the first time that rhOP-1 stimulates the proliferation of tendon cells and their ability to synthesize and accumulate PGs and collagen in their extracellular matrix. These biological properties may be used in the tissue-engineering of tendon tissues. ß

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Growth factors and canine flexor tendon healing: Initial studies in uninjured and repair models

Cited by 115 publications

References 20 publications

Tendon Healing with Growth Factors

Tendon Healing with Growth Factors

Ex vivo adenoviral transfer of bone morphogenetic protein 12 (BMP-12) cDNA improves Achilles tendon healing in a rat model

Effect of osteogenic protein‐1 on the matrix metabolism of bovine tendon cells

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