Growth factors in wound healing

Cross, Kevin J.; Mustoe, Thomas A.

doi:10.1016/s0039-6109(02)00202-5

Cited by 94 publications

(84 citation statements)

References 50 publications

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“…KGF-1, a member of the FGF family that is secreted by fibroblasts [Okazaki et al, 2002[Okazaki et al, , 2005, is a potent mediator of keratinocyte proliferation and migration [Cross and Mustoe, 2003]. Both keratinocytes and fibroblasts are important sources of bFGF (FGF-2), which stimulates proliferation, differentiation and migration of regenerated keratinocytes, aids angiogenesis by stimulating proliferation of capillary endothelial cells and promotes migration of fibroblasts [Bennet et al, 2003].…”

Section: Detection Of Selected Growth Factorsmentioning

confidence: 99%

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

Panacchia¹,

Dellambra²,

Bondanza³

et al. 2009

Cells Tissues Organs

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The standard method for producing graftable epithelia relies on the presence of a feeder layer of lethally irradiated 3T3-J2 murine fibroblasts (Rheinwald and Green technique). Here, we studied a new keratinocyte culture system, which envisages the utilization of nonirradiated human fibroblasts embedded into a fibrin substrate, in cultures destined for a future clinical application. We tested this culture system using keratinocytes grown on a fibrin gel precoated with 3T3-J2 murine fibroblasts as a control. In order to evaluate the new technology, we compared the clonogenic potential and the proliferative, differentiative and metabolic characteristics of keratinocytes cultured on the fibrin gel under the two culture conditions. The results demonstrated that the proposed technology did not impair the behavior of cultured keratinocytes and revealed that cells maintained their proliferative potential and phenotype under the experimental conditions. In particular, the demonstration of stem cell maintenance under the adopted culture conditions is very important for acute burn treatment with skin substitutes. This work is a first step in the evaluation of a new keratinocyte culture system, which has been studied in order to take advantage of an additional human cell population (i.e. nonirradiated, growing fibroblasts) for future transplantation purposes in acute and chronic wounds. Additional research will allow us to attain (1) the removal of murine cells in the initial phase of keratinocyte cultures, and (2) the removal of other potentially dangerous animal-derived materials from the entire culture system.

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Section: Detection Of Selected Growth Factorsmentioning

confidence: 99%

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

Panacchia¹,

Dellambra²,

Bondanza³

et al. 2009

Cells Tissues Organs

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“…The second, proliferative phase, lasting from the 4 th to the 14 th day after the injury, is focused on activation of keratinocytes, fibroblasts and endothelial cells. These cells are responsible for creating a scaffold in a healing wound and induction of neovascularization [5][6][7][8][9]. Additionally, various growth factors (IL-1, IL-6, GM-CSF, KGF, FGF, HGF, TGF-β) are secreted in vast amounts.…”

Section: Wound Healing -Stages and Critical Factorsmentioning

confidence: 99%

“…Three major groups of factors can be distinguished in proper wound healing: factors relying on the operational technique, factors dependent on the patient, and external factors [5][6][7][8][9]. Among the crucial elements involving surgical methods influencing wound healing is ischemia of wound edges or lack of appropriate wound homeostasis during the surgery.…”

Section: Wound Healing -Stages and Critical Factorsmentioning

confidence: 99%

“…Among the crucial elements involving surgical methods influencing wound healing is ischemia of wound edges or lack of appropriate wound homeostasis during the surgery. Additional agents with negative feedback are imperfect stitches [6]. Aging, obesity, diseases such as diabetes, renal insufficiency, inherited and acquired immune deficiencies and prolonged use of steroids may significantly impact the process of wound healing [6].…”

Section: Wound Healing -Stages and Critical Factorsmentioning

confidence: 99%

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Wound healing complications in oncological patients: perspectives for cellular therapy

Deptuła

Zieliński

Wardowska

et al. 2019

pdia

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A b s t r a c tVarious types of cancer are nowadays a serious medical and social problem and a great challenge for modern medicine. The majority of anticancer therapy is based on traditional chemotherapy and radiotherapy. Both of these highly non-specific types of treatment have a number of serious side effects including wound healing complications. Radiotherapy and chemotherapy mostly affect rapidly dividing skin cells (e.g. keratinocytes), as well as fibroblasts, melanocytes, endothelial and immune cells. Currently, there are many strategies to improve wound healing in oncological patients, including various types of dressings, biomaterials, growth factors, and cell therapies.

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“…Many studies support the use of hyperbaric oxygenation (HBO) as an adjuvant in the scarring of chronic wounds based on anecdotal experiences and, more recently, supported by clinical and laboratory findings of irritated wounds, in which HBO favors scarring and angiogenesis, improves leukocyte function and reduces edema (Cross andMustoe 2003, andRobson 2003). The effect of HBO on normal and cultured human skin cells was demonstrated in fibroblast growth decrease at pressures above 2.5 atm and in reduction of collagen production (Dimitrijevich et al 1999).…”

mentioning

confidence: 99%

Keloid skin scars: the influence of hyperbaric oxygenation on fibroblast growth and on the expression of messenger RNA for insulin like growth factor and for transforming growth factor

Romero‐Valdovinos¹,

Cárdenas-Mejía

Gutiérrez-Gómez

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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Wound healing can result in the development of keloid scars that contain atypical fibroblasts and an overabundance of extracellular matrix components. Hyperbaric oxygenation (HBO) refers to exposure to pure oxygen under increased atmospheric pressure and is recognized as a valuable supplementary method of treatment for problematic wounds. The effect of HBO in the expression of insulin-like growth factor type 1 (ILGF-1) and transforming growth factor β (TGF-β) messenger RNAs was determined by semiquantitative RT-PCR in fibroblasts obtained from keloid scars and nonwound involved skin fibroblast from the same patient. ILGF-1 and TGF-β are the principal mitogens during wound regeneration. We found a decrease in the growth of fibroblasts and in the expression of ILGF-1 and TGF-β messengers in keloid and nonkeloid fibroblast after chronic exposition to hyperbaric oxygenation compared with normal oxygen partial pressure.

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Growth factors in wound healing

Cited by 94 publications

References 50 publications

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

Wound healing complications in oncological patients: perspectives for cellular therapy

Keloid skin scars: the influence of hyperbaric oxygenation on fibroblast growth and on the expression of messenger RNA for insulin like growth factor and for transforming growth factor

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