Growth hormone augments superoxide anion secretion of human neutrophils by binding to the prolactin receptor.

Fu, Yung-Kang; Arkins, Seán; Fuh, Germaine; Cunningham, Brian C.; Wells, James A.; Fong, Sherman; Cronin, Michael J.; Dantzer, Robert; Kelley, Keith W.

doi:10.1172/jci115605

Cited by 116 publications

(41 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD11b/CD18 acts as counterreceptor for OPZ (i.e., iC3b [20,36]) and mediates O 2 Ϫ production (6,20), and the enhanced O 2 Ϫ production in response to the OPZ further suggests that the activity of CD11b/CD18 is increased following GH priming. Other investigators (18,19) have also investigated the O 2 Ϫ produced extracellularly by human PMN following GH priming, and the magnitude by which human GH enhanced the O 2 Ϫ production was far higher than that in our results. The difference in magnitude is presumably due to the fact that these investigators measured the extracellularly produced O 2 Ϫ and also to the fact that the PMN were primed for 3 to 4 h.…”

Section: Discussioncontrasting

confidence: 74%

Characterization of the Priming Effect by Pituitary Canine Growth Hormone on Canine Polymorphonuclear Neutrophil Granulocyte Function

Petersen¹,

Smith

Jensen³

2000

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

In this report, we demonstrate that canine growth hormone (cGH) is capable of priming canine polymorphonuclear neutrophil granulocytes (PMN) in a manner resembling that of human PMN. The cGH influences important functions that are involved in the process of recruitment of PMN, i.e., shape change, chemotaxis, CD11b/CD18 expression, adhesion, and subsequent transendothelial migration. Also, intracellular O 2 ؊ production was evaluated. We investigated the priming effect by incubating PMN with purified pituitary cGH at various concentrations (10 to 800 g/liter). The capacity for shape change was significantly (P < 0.05) enhanced, whereas the chemotactic response under agarose was significantly (P < 0.05) reduced. The chemotactic migration in Boyden chambers (10-m-thick polycarbonate filter; lower surface count technique) was significantly (P < 0.05) enhanced, presumably due to cGH-induced hyperadhesiveness to the lower surface of the filters. The adhesion in albumin-coated microtiter plates and adherence to canine pulmonary fibroblasts were significantly (P < 0.05) increased, and the increased adhesion resulted in a significant (P < 0.01) increase in transendothelial migration using canine jugular vein endothelial cells. The increase in adhesion was associated with a significant increase in CD11b/CD18 expression. Furthermore, intracellular O 2 ؊ production was significantly enhanced in response to both phorbol myristate acetate (P < 0.01) and opsonized zymosan (P < 0.05). In the absence of a PMN-stimulating agent, cGH did not influence the effector functions investigated except for an increased expression of CD11b/CD18.Polymorphonuclear neutrophil granulocytes (PMN) play an important role in organisms' first line of defense against invading agents (25,42). Upon activation in the bloodstream, the PMN become more adhesive, allowing receptor-mediated margination and adhesion to the vasculature (26, 40-42, 44, 51, 58). Then, adherent PMN undergo shape change and crawl on the surface of the endothelial cells, followed by transendothelial migration into the extracellular compartment (5, 40-42, 44, 51). Subsequently, PMN migrate along a chemotactic gradient toward the offending stimulus to finally kill the invading agent by phagocytosis or release of granule contents and reactive oxygen metabolites (2,25,42,56).Growth hormone (GH) has been identified as a factor involved in the regulation of PMN function by priming (17-19, 28, 37-39, 43, 52, 54), whereby the response of PMN to an activating stimulus is potentiated (7). Except for one report (37), previous work shows that GH primes human (18,19,28,39,43,52), porcine (18,19), and bovine (18) PMN in vitro for an enhanced respiratory burst and a reduced chemotactic migration of human PMN (17, 52, 54), which has been suggested to be due to GH-induced hyperadhesiveness (52). These findings correlate with observations for human acromegalic patients, in whom the respiratory burst is increased (38) and the chemotactic response is decreased (16). In contrast to the findings for ...

show abstract

Section: Discussioncontrasting

confidence: 74%

Characterization of the Priming Effect by Pituitary Canine Growth Hormone on Canine Polymorphonuclear Neutrophil Granulocyte Function

Petersen¹,

Smith

Jensen³

2000

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

show abstract

“…More recently, however, it has been shown that macrophage activation (408) and superoxide anion production responsible for killing pathogenic organisms (466,467) are effects mediated by the PRLR. The protective effect against Salmonella infection appears to be mediated by nitric oxide production (468).…”

Section: F Immunoregulation and Protectionmentioning

confidence: 99%

Prolactin (PRL) and Its Receptor: Actions, Signal Transduction Pathways and Phenotypes Observed in PRL Receptor Knockout Mice

1998

View full text Add to dashboard Cite

“…Elvinger et al [71] showed that administration of rbST to cows increased lymphocyte blastogenesis but not phagocytosis and killing of E. coli by PMN. Recombinant human growth hormone primes human neutrophils for enhanced superoxide anion secretion [80].…”

Section: Effect Of Rbst On Milk Loss During Mastitismentioning

confidence: 99%

Severity of E. coli mastitis is mainly determined by cow factors

et al. 2003

View full text Add to dashboard Cite

-Intramammary infections of dairy cows with Gram-positive bacteria such as Staphylococcus aureus (major cause of mastitis) have received a lot of attention because of their major economic impact on the dairy farm through production losses induced by an increase in somatic cell count. Management strategies, including greater awareness for efficient milking and hygienic measures, have limited the spread of Gram-positive bacteria and resulted in a significant decrease of proportion of S. aureus isolates and subclinical mastitis worldwide. Other organisms such as coliform subspecies and Streptococcus uberis, both environmental bacteria that cause clinical mastitis, have received less attention. Escherichia coli causes inflammation of the mammary gland in dairy cows around parturition and during early lactation with striking local and sometimes severe systemic clinical symptoms. This disease affects many high producing cows in dairy herds and may cause several cases of death per year in the most severe cases. It is well known that bacterial, cow and environmental factors are interdependent and influence mastitis susceptibility. Many studies, executed during the last decade, indicate that the severity of E. coli mastitis is mainly determined by cow factors rather than by E. coli pathogenicity. During E. coli mastitis, the host defense status is a cardinal factor determining the outcome of the disease. Today, we know that the neutrophil is a key factor in the cows' defense against intramammary infection with E. coli. Effective elimination of the pathogen by neutrophils is important for the resolution of infection and the outcome of E. coli mastitis. This review is a compilation of some major findings over the last 15 years concerning mainly host factors that modulate and influence neutrophil function and the mammary inflammatory reaction. The individual chapters address: virulence factors of E. coli strains, how neutrophils kill E. coli, connection between endotoxins, tumor necrosis factor-a and nitric oxide, severity classification of E. coli mastitis, lifespan of neutrophils, host factors that influence severity, tissue damage and production loss.

show abstract

Growth hormone augments superoxide anion secretion of human neutrophils by binding to the prolactin receptor.

Cited by 116 publications

References 51 publications

Characterization of the Priming Effect by Pituitary Canine Growth Hormone on Canine Polymorphonuclear Neutrophil Granulocyte Function

Characterization of the Priming Effect by Pituitary Canine Growth Hormone on Canine Polymorphonuclear Neutrophil Granulocyte Function

Prolactin (PRL) and Its Receptor: Actions, Signal Transduction Pathways and Phenotypes Observed in PRL Receptor Knockout Mice

Severity of E. coli mastitis is mainly determined by cow factors

Contact Info

Product

Resources

About