Growth hormone binding to tissues of normal and stunted juvenile coho salmon, Oncorhynchus kisutch

Abstract

The premature transfer ofjuvenile Coho salmon into seawater results in high mortality and a reduction of growth (stunting) of a significant portion of the population while the remainder of the salmon continue to grow normally. Binding studies were conducted with 1251-labelled tilapia (Sarotherodon mossambicus) growth hormone (tGH) to determine if differences in specific binding of tGH existed in membrane preparations of tissues of stunted and normal coho salmon. Membrane preparations ofboth liver and gill of stunted salmon obtained from stocks in California, Washington and British Columbia and kidney membrane preparations obtained from stunted salmon in California exhibited deficiencies in 1*Wabelled tGHbinding sites compared to corresponding membrane preparations of normal smolts. These results indicate that stunted coho salmon have a decreased ability to bind GH which may be reflected in perturbations in GH-mediated processes.

Effects of acute and chronic stress on the levels of circulating growth hormone in the rainbow trout, Oncorhynchus mykiss

“…A similar correlation between reduced growth rate and blood GH elevation has been observed in the phenomenon of "stunting" in both coho and Atlantic salmon, Salmo s&r, smolts (Bolton et al, 1987;Bjornsson et al, 1988). However, the problem of suppressed growth in such stunts is thought to be related to target-tissue sensitivity rather than the rate of pituitary GH secretion (Fryer and Bern, 1979). Clearly much more information is now needed concerning the mechanisms of action of salmonid growth hormone in both normal and chronically stressed fish and the possible role of insulin-like growth factors or somatomedins in mediating these effects.…”

Presence of specific growth hormone binding sites in rainbow trout (Oncorhynchus mykiss) tissues: Characterization of the hepatic receptor

“…data), this variability can be explained in part by the different sexual stages and degrees of nutrition of the animal used in this study. Fryer and Bern (1979) and more recently Gray et al (1989) demonstrated that specific binding of GH is lower in stunted than in normal coho salmon. In mammals, variations of GH binding were also observed during pregnancy (Posner, 1976;Gerasimo et al, 1979) and starvation (see Postel-Vinay et al, 1987).…”

“…Using this last hormone, specific binding was also found in the liver of mudsucker, rainbow trout (Oncorhynchus mykiss), and chinook salmon (0. tshawytschu) (Fryer, 1979b), and one study indicated that stunted coho salmon (0. kisutch) have a decreased ability to bind GH by liver (Fryer and Bern, 1979). More recently, using chinook salmon GH, we demonstrated the presence of saturable binding in rainbow trout liver (Le Bail et al, 1989;Niu et al, submitted for publication).…”

Development of a protein binding assay for teleost insulin-like growth factor (IGF)-like: relationships between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss)

“…Their number are modulated by stunting (Fryer and Bern 1979;Gray et al 1990), salinity (Sakamoto and Hirano 1991), GH plasma levels, temperature or starvation (P6rez-Sanchez et al 1991;Yao and Le Bail, unpublished data). However, GH receptors may not the only element involved in the receptivity of target tissues to GH.…”