Growth Hormone-Dependent Effects of Human Serum on the<i>in Vitro</i>Growth Characteristics of Human Skin Fibroblasts

MacGillivray, Margaret H.; Hastings, Claudia; Brown, J. Anne

doi:10.1210/jcem-40-1-62

Cited by 10 publications

(3 citation statements)

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“…Previous experimental work has demonstrated that growth hormone is essential for the in vitro growth of human skin fibroblasts [13]. Clinically, proliferation of dermal fibroblasts and excessive collagen production arc observed in acromegaly [14].…”

Section: Discussionmentioning

confidence: 99%

Coexistent Urticaria pigmentosa, Acromegaly and Acanthosis nigricans

Akiyama

Sasaki²,

Takahashi³

et al. 1991

Dermatology

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We report a case of urticaria pigmentosa, acromegaly and acanthosis nigricans in a 25-year-old male. The patient exhibited multiple pigmented papules on the trunk and the extremities. Histological examinations of the papules revealed infiltrates of mast cells in the upper dermis. Ultrastructurally the mast cells were fully matured and exhibited no atypical features. A typical appearance of acromegaly, frontal bossing, prominence of the jaw and bony overgrowth and cutaneous changes of acanthosis nigricans on the neck, the axillae and the groins were observed. Growth hormone hypersecretion and insulin resistance were detected in the patient. A pituitary tumor was found and resected surgically. After the operation, endocrinological abnormalities and cutaneous manifestations of acanthosis nigricans improved markedly. As far as we know, this is the first report of the coexistence of urticaria pigmentosa, acromegaly and acanthosis nigricans.

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Section: Discussionmentioning

confidence: 99%

Coexistent Urticaria pigmentosa, Acromegaly and Acanthosis nigricans

Akiyama

Sasaki²,

Takahashi³

et al. 1991

Dermatology

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“…Previous experimental work has emphasized fibroblastie proliferation and increased production of collagen as effeets of growth hormone on the skin. The effects of growth hormone on fibroblast function have been elegantly demonstrated using confluent monolayer, and serum from hGH deficient children laeks a growth hormone depeiidenl factor (presumably somatomedin) which is essential for the //; vitro growth of human skin fibroblasts (MaeGillivray et al 1975).…”

Section: Discussionmentioning

confidence: 99%

Dermal Changes in Osteoporosis Following Prolonged Treatment with Human Growth Hormone

Aloia

Grover

1976

J Cutan Pathol

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Five patients with osteoporosis were treated with human growth hormone (hGH) for a year and the changes in their skin were studied by light and electron microscopy. The abnormally thin skin of osteoporosis appeared to change towards normal after treatment with hGH. There was a consistent proliferation of blood vessels, and increased number of mast cells and fibrocytes. The collagen bundles and elastic tissue fibers appeared hyperplastic and more horizontally oriented. The fine, vertical elastic fibrils of the papillary dermis had appeared decreased before treatment, but seemed to be restored to their normal configuration after treatment. Since there was no evidence of stimulation of hair, sebum, or melanin such as occurs in acromegaly, it is suggested that the scope of the direct action of hGH on the skin is limited to mesenchymal structures.

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“…Conflicting reports appear in the literature regarding the somatomedin dependence of human skin fibroblast proliferation. Using various parameters of cell growth and different culture conditions, investigators have reported that SM-depleted serum from hypopituitary children is less potent (MacGillivray, Hastings and Brown 1975;Nakazawa, Okada, Kato, Suzuki and Kumahara 1981), or equipotent (Moses, Cohen, Johnsonbaugh and Nissley 1978;August, Cheng, Hung and Houck 1973;Clemmons and Van Wyk 1981) with serum from GHtreated hypopituitary or normal children. In addition serum from hypox rats has been reported to be more potent than normal rat serum in stimulating DNA synthesis in human fibroblasts (Olsen, Patton, Martin and Wangsness 1979).…”

Section: Introductionmentioning

confidence: 99%

Effects of Hypophysectomy and Subsequent Growth Hormone Replacement in the Rat on the Ability of Serum to Stimulate Proliferation of Human and Mouse (BALB/c 3T3) Fibroblasts

Lj¹,

Lazarus²

1984

Horm Metab Res

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The ability of cultured human fibroblasts to synthesize and secrete a mitogenic, somatomedin C-like peptide suggests that human fibroblasts unlike certain other cell lines may not require exogenous somatomedin for cell proliferation. The aim of this study was to examine the effects of hypophysectomy and subsequent growth hormone (GH) replacement on the ability of rat serum to stimulate proliferation and DNA synthesis in cultured human fetal skin fibroblasts. For comparison we have also examined the effects of serum from hypophysectomized (hypox) and GH-replaced hypox rats on mouse BALB/c 3T3 fibroblasts, a cell line which has been shown to require exogenous somatomedin (SM) for DNA synthesis and cell proliferation. Human fibroblast proliferation and DNA synthesis (measured as thymidine incorporation) were significantly enhanced (P less than 0.025) when these cells were cultured in low concentrations of hypox rat serum compared to normal rat serum. When thymidine incorporation into DNA was expressed in terms of a normal rat serum pool (arbitrarily assigned a potency of 1 unit/ml), serum from hypox rats was significantly more potent than serum from normal rats (1.52 +/- 0.16 (SEM) vs. 0.99 +/- 0.10 units/ml, P less than 0.0125). Replacement treatment of hypox rats with human GH, 0.2 IU/100 g body weight/day or 0.4 IU/100 g body weight/day decreased the potency of the rat serum in terms of stimulation of thymidine incorporation from 1.52 +/- 0.16 to 1.30 +/- 0.14 and 1.08 +/- 0.06 units/ml respectively. In contrast serum from untreated hypox rats was significantly less potent than normal rat serum in stimulating BALB/c 3T3 DNA synthesis (0.36 +/- 0.06 vs. 1.04 +/- 0.16 units/ml, P less than 0.0025).(ABSTRACT TRUNCATED AT 250 WORDS)

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Growth Hormone-Dependent Effects of Human Serum on thein VitroGrowth Characteristics of Human Skin Fibroblasts

Cited by 10 publications

References 0 publications

Coexistent Urticaria pigmentosa, Acromegaly and Acanthosis nigricans

Coexistent Urticaria pigmentosa, Acromegaly and Acanthosis nigricans

Dermal Changes in Osteoporosis Following Prolonged Treatment with Human Growth Hormone

Effects of Hypophysectomy and Subsequent Growth Hormone Replacement in the Rat on the Ability of Serum to Stimulate Proliferation of Human and Mouse (BALB/c 3T3) Fibroblasts

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