Growth hormone does not attenuate the inhibitory effects of sepsis on wound healing

Stamm, Jason; Cooney, Robert N.; Maish, George O.; Shumate, Margaret L.; Lang, Charles H.; Ehrlich, H. Paul; Vary, Thomas C.

doi:10.1046/j.1524-475x.2000.00103.x

Cited by 12 publications

(13 citation statements)

References 26 publications

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“…In this review, 43 of the 55 studies were identified that had wounds placed on the dorsum of the rats 6,9–13,18–54 . There was an overlap in categorizing the groups according to wound placement because wounds were made in different areas within the same study; for example, wounds were made on the dorsum and the ventral portion of rats 53 . Exact statements as to locations and depths of wounds were not always readily available, leaving the reader to draw assumptions from both the text and figures 55 .…”

Section: Wound Locationmentioning

confidence: 99%

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Rat models of skin wound healing: A review

Dorsett‐Martin

2004

Wound Repair Regeneration

286

144

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Rats have been widely used in the study of skin wound healing and the efficacy of different treatment modalities. This particular animal species is often selected for its availability, low cost, and small size. To define the current use of rat skin wound healing models, this manuscript provides a review of articles published between 2000 and 2003 that chose rats as their research animals. Of the 55 articles reviewed, it was found that 38.2% of the studies used incisional models and 38.2% used excisional models, with some studies using combinations. The majority of the studies (78.2%) used the rat's dorsum as the wound location. Male Sprague Dawley in the 250-300 gram weight range were the most preferred rats. Sodium pentobarbital/ pentobarbitone was the most commonly used anesthetic choice. Similarities and differences in the selected experimental conditions are noted and questions are raised with regard to comparability between studies and the ability to transfer the data from the animal model to the human clinical situation. Attempts to compare studies for the advancement of wound healing knowledge are being hampered by the differences found between the studies. Standardization in reporting could facilitate comparisons and may instigate additional research that favors the inevitable comparisons between the studies. Thus, universal reporting requirements need to be developed for animal wound healing studies. (WOUND REP REG 2004;12:591-599)

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Section: Wound Locationmentioning

confidence: 99%

“…Details on the research animals are included in Table 4. Thirty‐one of the 55 studies used the Sprague‐Dawley strain of rat 5,6,9–13,15,19–22,24,26,28,31–33,36,37,40,44,46,48,51,53,59, 60,68–70 . The second most popular was the Wistar strain with 15 studies 14,16,17,25,27,29,30,34,39,41,43,49,50,52,55 .…”

Section: Strain and Sex Of Ratsmentioning

confidence: 99%

Rat models of skin wound healing: A review

Dorsett‐Martin

2004

Wound Repair Regeneration

286

144

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“…However, the effects of GH on collagen formation are probably tissue‐specific. GH treatment in rats did not increase collagen deposition in cutaneous wounds 31 . Hypertrophic scars have not been reported in acromegaly patients, whose condition is characterized by overproduction of GH.…”

Section: Discussionmentioning

confidence: 72%

Growth hormone effects on hypertrophic scar formation: a randomized controlled trial of 62 burned children

Oliveira

Sanford

Murphy

et al. 2004

Wound Repair Regeneration

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The hypercatabolism after massive pediatric burns has been effectively treated with recombinant human growth hormone, an anabolic agent that stimulates protein synthesis and abrogates growth arrest. While experimental studies have shown increased potential for fibrosis induced by growth hormone therapy, adverse effects on human scars have not been investigated. Our aim was to evaluate hypertrophic scar formation in 62 patients randomized to receive injections of 0.05 mg/kg/day of recombinant human growth hormone or placebo, from discharge until 1 year after burn. Scar scales were used to evaluate scar-severity at discharge, 6, 9, 12, and 18-24 months after burn, by three observers blinded to treatment. Computer-assisted planimetry allowed quantification of percentage of hypertrophic scar formation. Types I and III collagens were localized and quantified in scars and normal skin of patients from both groups, using immunohistochemistry with confocal laser microscopy analysis. Insulin-like growth factor-1 blood levels helped assess compliance. Statistical analysis showed that scar hypertrophy significantly increased from 6 to 12 months after injury in both groups, while decreasing at 18-24 months postburn. Types I and III collagens were statistically increased in the reticular layer of scars from both groups when compared to paired normal skin. Insulin-like growth factor-1 was significantly increased in the recombinant human growth factor-treated group. No differences were seen when recombinant human growth factor and control groups were compared using the scar scales, planimetry, or immunohistochemistry. We concluded that recombinant human growth hormone therapy did not adversely affect scar formation and should not contraindicate the administration of recombinant human growth hormone as a therapeutic approach to severely burned children.

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“…The effect of GH on wound healing in sepsis was controversial [27][28][29][30]. Stamm et al [10] had found that breaking strength of incisional wounds and hydroxyproline content were reduced in septic rats, administration of GH for 5 days did not improve breaking strength or collagen deposition in septic animals, so the investigators believed that GH is unlikely to improve tissue repair in sepsis-induced catabolic illness. In this study, we found that administration of rhGH for 5 days fail to improve intestinal healing in intra-abdominal sepsis, the combination of FG and rhGH have no advantage over using FG alone in improving intestinal healing.…”

Section: Discussionmentioning

confidence: 99%

“…Stamm and his colleagues, however, had demonstrated that the administration of GH for 5 days did not improve collagen deposition or breaking strength in cutaneous wounds in septic rats [10]. The effect of GH on intestinal anastomotic healing in intra-abdominal sepsis has not been known.…”

Section: Introductionmentioning

confidence: 99%