Growth hormone (GH) status regulates GH receptor and GH binding protein mRNA in a tissue- and transcript-specific manner but has no effect on insulin-like growth factor-I receptor mRNA in the rat

Butler, Andrew A.; Funk, B.; Breier, Bernhard H.; LeRoith, Derek; Roberts, Charles T.; Gluckman, Peter D.

doi:10.1016/0303-7207(95)03713-6

Cited by 25 publications

(17 citation statements)

References 31 publications

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“…This is unlike the liver, where levels of GHR and GHBP are approximately equal. Butler et al (1996) have also found that various organs have different mRNA ratios of GHR to GHBP. They found that when the GH-deficient rat was compared with normal controls, GHBP and GHR expression were decreased in liver.…”

Section: Discussionmentioning

confidence: 93%

“…Single-stranded 32 P-labelled probes for IGF-I (Lowe et al 1988), for cyclophilin (Jakubowski et al 1991) and for GHR/GHBP (Butler et al 1996) were used in an RNAse protection assay as described previously (Batchelor et al 1995, Butler et al 1996. RNAse digestion resulted in fragments of 224 bases for the IGF-I, 300 bases for cyclophilin and 439 and 290 bases corresponding to full length GHR and GHBP mRNA respectively.…”

Section: Labelled Probesmentioning

confidence: 99%

“…RNAse protection assays were carried out as described previously (Batchelor et al 1995, Butler et al 1996. Briefly, aliquots (20 µg) were hybridised with an excess of each labelled probe.…”

Section: Rna Analysis Of Whole Lung and Cell Culturementioning

confidence: 99%

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Fetal rat lung epithelium has a functional growth hormone receptor coupled to tyrosine kinase activity and insulin-like growth factor binding protein-2 production

Batchelor

Lewis²,

Breier³

et al. 1998

Journal of Molecular Endocrinology

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Although growth hormone (GH) receptor (GHR) mRNA and protein are present in fetal tissues such as the lung, there is little evidence that GH mediates growth in the fetus. We have identified functional responses to GH in fetal rat lung epithelia and suggest a possible role for GHR in the developing lung. GHR mRNA in lung extracts was high before birth at day 16 of gestation (16f), decreased to low levels at day 22f but increased again after birth. At day 20f GHR mRNA levels were higher in lung than in liver, whereas growth hormone binding protein mRNA levels were approximately equal in lung and liver. Stimulation of primary cell cultures of day 19f lung epithelia with GH caused increased tyrosine phosphorylation in specific proteins, demonstrating functional GHR. Lung fibroblasts isolated at the same time did not respond to GH. Ligand and Northern blot analysis of the epithelial cultures revealed that GH stimulation increased insulin-like growth factor binding protein-2 (IGFBP-2) activity and mRNA. These experiments demonstrate the functional activity of GHR, specifically in fetal lung epithelium. We suggest that one role for GH in vivo may be indirectly to modify insulin-like growth factor activity in the developing fetal lung by increasing IGFBP-2.

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Section: Discussionmentioning

confidence: 93%

Section: Labelled Probesmentioning

confidence: 99%

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Fetal rat lung epithelium has a functional growth hormone receptor coupled to tyrosine kinase activity and insulin-like growth factor binding protein-2 production

Batchelor

Lewis²,

Breier³

et al. 1998

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…However, some possible factors are considered to be the candidates; these include insulin [3,19], glucocorticoids [4,20] and GH [5,[21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

“…The number and function of GHR affect the ability of a tissue to respond to GH [2]. GHR messenger ribonucleic acid (mRNA) expression has been reported to be regulated by various factors, including insulin [3], glucocorticoids [4], GH [5] and nutritional status [6], and the regulation is tissue specific. It is known that there is a circadian pattern of circulating cortisol in rodents and humans [7,8] and GH secretion is pulsatile in these species [9,10].…”

mentioning

confidence: 99%