Growth inhibition and apoptosis induction by Scutellaria pinnatifida A. Ham. on HL-60 and K562 leukemic cell lines

Boozari, Motahareh; Mohammadi, Ameneh; Asili, Javad; Emami, Seyed Ahmad; Tayarani‐Najaran, Zahra

doi:10.1016/j.etap.2014.12.002

Cited by 32 publications

(26 citation statements)

References 21 publications

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“…Semipolar nature of MeOH and CH 2 Cl 2 may extract phytochemicals which are responsible for antimelanogenic activity. Although the n -hexane fraction could decrease ROS and cell tyrosinase activity but it could not show any inhibitory effect on melanin content which may indicate that plant phytochemicals with nonpolar nature have not significant effect on the melanogenesis process(2122). …”

Section: Discussionmentioning

confidence: 93%

Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress

et al. 2017

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Nepeta satureioides Boiss. has been used in traditional medicine of eastern countries and is famous for its medicinal properties. The aim of this study was to evaluate the effect of methanol (MeOH), n-hexane and dichloromethane (CH2Cl2) fractions of the extract on melanin synthesis and oxidative stress in B16F10 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different fractions of the plant (5-60 μg/mL) was measured using MTT assay. The inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and oxidative stress were determined by the colorimetric and fluorometric methods. The data showed that at concentrations below 60 μg/mL, fractions did not show significant toxicity on melanoma cells. The amount of melanin synthesis by MeOH and CH2Cl2 fractions and mushroom tyrosinase activity by the MeOH fraction declined in B16F10 cells. In addition to the capacity of MeOH, n-hexane and CH2Cl2 fractions in decreasing the amount of reactive oxygen species (ROS) in melanoma cells, all fractions revealed remarkable antioxidant activity. The melanogenesis inhibitory and antioxidant effects of N. satureioides on B16F10 cells may suggest this plant as a new pharmaceutical agent in reducing skin pigment and skin aging in cosmetic industry.

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Section: Discussionmentioning

confidence: 93%

Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress

et al. 2017

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“…Additionally, two important flavonoids, wogonin and skullcap flavone II, which have been identified in the roots of S . pinnatifida [ 60 , 61 ] exhibit significant anti-inflammatory effects [ 26 , 62 ]. The anti-inflammatory activity of wogonin is connected with its ability to inhibit NF-κB pathway [ 26 – 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…There is little information on the pharmaceutical properties of the Iranian species, Scutellaria pinnatifida (S . pinnatifida ) [ 30 – 32 ]. It was determined that among 60 different Iranian herbs only 6 S .…”

Section: Introductionmentioning

confidence: 99%

“…pinnatifida , it was shown that the methanolic extract (MeOHEx) had more antibacterial activity than the DCM extract (DCMEx), but DCMEx exhibited more antioxidant activity [ 32 ]. It was also indicated that its root CH 2 Cl 2 extraction induced specifically apoptosis in some cancer cell lines [ 30 ]. However, its activity against α-SN fibrillation and its neurotoxicity or its protective effect against environmental toxins has never been investigated before.…”

Section: Introductionmentioning

confidence: 99%

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A study on the modulation of alpha-synuclein fibrillation by Scutellaria pinnatifida extracts and its neuroprotective properties

et al. 2017

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Aggregation of alpha-synuclein (α-SN) is a key pathogenic event in Parkinson’s disease (PD) leading to dopaminergic degeneration. The identification of natural compounds inhibiting α-SN aggregation may have a major role in treating PD. Different Scutellaria species are known as valuable medicinal plants, primarily due to their high flavonoid levels. Scutellaria pinnatifida (S. pinnatifida) is endemic to Iran; however, the knowledge of its pharmaceutical properties is limited. Here we report that S. pinnatifida extracts have an anti-fibrillation effect on α-SN aggregation and neuroprotective properties on PC12 and primary dopaminergic neurons. Treatment during α-SN fibril formation with S. pinnatifida extracts showed that the extractions performed with dichloromethane (DCMEx) and n-butanol (BuOHEx) strongly inhibited α-SN fibrillation. TLC-based analysis revealed that S. pinnatifida contains a great amount of flavonoids with high antioxidant properties as shown using a radical scavenging assay. Further analysis using HPLC and Mass spectroscopy on the DCMEx revealed the presence of baicalein in this extract. We then selected the more efficient extracts based on cell viability and ROS scavenging on PC12 cells and tested their neuroprotective properties on primary dopaminergic neurons. Our results showed the extracts strongly protected against α-SN oligomers. Surprisingly, they also neutralized the severe toxicity of paraquat. Therefore, S. pinnatifida may be a potential valuable medicinal herb for further studies related to the treatment of PD.

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“…More interestingly, SKF-II was found to poses previously unreported antimalarial activity superior to wogonin with an IC50=2.8µg/mL, which would be an equivalent of ~7.5µM for the pure compound. Whilst both compounds have not been pursued so far as candidate drugs for malaria, their anti-cancer and anti-metastatic activities have been well-established and demonstrated both in vitro and in vivo on multiple cancer lines and mouse tumour models(288)(289)(290)(291)(292). Numerous studies have shown that wogonin preferentially kills tumour cells, evoking none or little toxicity to normal tissues(293- 296) making it a good drug candidate.…”

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confidence: 99%

Molecular analysis of antimalarial therapies : from drug development to mode of action

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Growth inhibition and apoptosis induction by Scutellaria pinnatifida A. Ham. on HL-60 and K562 leukemic cell lines

Cited by 32 publications

References 21 publications

Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress

Inhibitory effects of different fractions of Nepeta satureioides on melanin synthesis through reducing oxidative stress

A study on the modulation of alpha-synuclein fibrillation by Scutellaria pinnatifida extracts and its neuroprotective properties

Molecular analysis of antimalarial therapies : from drug development to mode of action

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