Growth inhibition and induction of differentiation by panaxydol in rat C6 glioma cells

Hai, Jian; Lin, Qi; Yang, Lu; Yi, Jing; Zhang, Hao

doi:10.1179/016164107x228697

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…Essentially, falcarinol eliminated CSC population in NSCLC and abolished lung tumor formation in mice via HSP90 (a molecular chaperone of numerous oncoproteins) modulation [302]. Falcarinol anticancer activity also extends to leukemia [303], breast cancer [304], hepatocarcinoma [305], renal carcinoma [306], and glioma [307]. For instance, mechanisms pointed to explain its ability to induce cell cycle arrest, thus, its anticarcinogenic properties on human promyelocytic leukemia cell growth are PKCδ proteolytic cleavage, caspase-3 activation, and PARP degradation [303].…”

Section: Falcarinolmentioning

confidence: 99%

Terpenoids, Cannabimimetic Ligands, beyond the Cannabis Plant

et al. 2020

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Medicinal use of Cannabis sativa L. has an extensive history and it was essential in the discovery of phytocannabinoids, including the Cannabis major psychoactive compound—Δ9-tetrahydrocannabinol (Δ9-THC)—as well as the G-protein-coupled cannabinoid receptors (CBR), named cannabinoid receptor type-1 (CB1R) and cannabinoid receptor type-2 (CB2R), both part of the now known endocannabinoid system (ECS). Cannabinoids is a vast term that defines several compounds that have been characterized in three categories: (i) endogenous, (ii) synthetic, and (iii) phytocannabinoids, and are able to modulate the CBR and ECS. Particularly, phytocannabinoids are natural terpenoids or phenolic compounds derived from Cannabis sativa. However, these terpenoids and phenolic compounds can also be derived from other plants (non-cannabinoids) and still induce cannabinoid-like properties. Cannabimimetic ligands, beyond the Cannabis plant, can act as CBR agonists or antagonists, or ECS enzyme inhibitors, besides being able of playing a role in immune-mediated inflammatory and infectious diseases, neuroinflammatory, neurological, and neurodegenerative diseases, as well as in cancer, and autoimmunity by itself. In this review, we summarize and critically highlight past, present, and future progress on the understanding of the role of cannabinoid-like molecules, mainly terpenes, as prospective therapeutics for different pathological conditions.

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Section: Falcarinolmentioning

confidence: 99%

Terpenoids, Cannabimimetic Ligands, beyond the Cannabis Plant

et al. 2020

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“…Cells treated with 60 lM panaxydol for 48 h were therefore used for subsequent experiments. 7,11 Briefly, 2.5 Â 10 3 C6 cells were seeded in 96-well microtiter plates (Nune, Roskilde, Denmark), incubated at 37°C for 24 h, and grown with or without 5 lM wortmannin for 4 h; 60 lM panaxydol was added to the medium. Cells were harvested after 48 h. MTT (10 lL) was added to each well, and cells were incubated at 37°C for 4 h. Absorbance at 570 nm was read on a microplate reader against a reference wavelength of 630 nm.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

“…These aliphatic C17 polyacetylenes were isolated from the lipophilic fractions of a well-known herb in Chinese traditional medicine, Tienchi ginseng (Panax notoginseng). [7][8][9] The molecular mechanisms underlying this differentiation are unknown. In this study, we propose that PI 3-K activity is required for the regulation of differentiation in C6 cells treated with panaxydol.…”

Section: Introductionmentioning

confidence: 99%

Phosphatidylinositol 3-kinase activity is required for the induction of differentiation in C6 glioma cells by panaxydol

Hai

Lin

Yang

2009

Journal of Clinical Neuroscience

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“…Recently, agents extracted from Chines traditional medicine have been reported to have therapeutic effects against gliomas. For examples, Jian et al reported that panaxydol, isolated from the lipophilic fractions of Panax notoginseng, a well-known Chinese traditional medicine, inhibited the proliferation of C6 cells in a dose-dependent manner and induced p27 expression and differentiation in rat C6 glioma cells [6]. Shao et al reported that nanoparticles loaded with Curcumina, yellow pigment in the spice turmeric displayed pro-apoptosis effect against C6 cells [7].…”

Section: Introductionmentioning

confidence: 99%

Artemether Combined with shRNA Interference of Vascular Cell Adhesion Molecule-1 Significantly Inhibited the Malignant Biological Behavior of Human Glioma Cells

Wang

Hu²,

Li³

et al. 2013

PLoS ONE

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Artemether is the derivative extracted from Chinese traditional herb and originally used for malaria. Artemether also has potential therapeutic effects against tumors. Vascular cell adhesion molecule-1 (VCAM-1) is an important cell surface adhesion molecule associated with malignancy of gliomas. In this work, we investigated the role and mechanism of artemether combined with shRNA interference of VCAM-1 (shRNA-VCAM-1) on the migration, invasion and apoptosis of glioma cells. U87 human glioma cells were treated with artemether at various concentrations and shRNA interfering technology was employed to silence the expression of VCAM-1. Cell viability, migration, invasiveness and apoptosis were assessed with MTT, wound healing, Transwell and Annexin V-FITC/PI staining. The expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and phosphorylated Akt (p-Akt) was checked by Western blot assay. Results showed that artemether and shRNA-VCAM-1 not only significantly inhibited the migration, invasiveness and expression of MMP-2/9 and p-Akt, but also promoted the apoptosis of U87 cells. Combined treatment of both displayed the maximum inhibitory effects on the malignant biological behavior of glioma cells. Our work revealed the potential therapeutic effects of artemether and antiVCAM-1 in the treatments of gliomas.

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Growth inhibition and induction of differentiation by panaxydol in rat C6 glioma cells

Abstract: These findings suggest that panaxydol inhibits the proliferation of C6 cells via G0/G1 cell cycle arrest in association with induction of p27 expression and differentiation.

Cited by 9 publications

References 24 publications

Terpenoids, Cannabimimetic Ligands, beyond the Cannabis Plant

Terpenoids, Cannabimimetic Ligands, beyond the Cannabis Plant

Phosphatidylinositol 3-kinase activity is required for the induction of differentiation in C6 glioma cells by panaxydol

Artemether Combined with shRNA Interference of Vascular Cell Adhesion Molecule-1 Significantly Inhibited the Malignant Biological Behavior of Human Glioma Cells

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