Growth  Inhibition  of  Colletotrichum  gloeosporioides  by  Trichoderma harzianum,  Trichoderma  koningii,  Bacillus  subtilis and  Pseudomonas  fluorescens

Aini, Febrilia Nur; Sukamto, Sri; Wahyuni, Dwi; Suhesti, Risma Galuh; Ayunin, Qurrotun

doi:10.22302/iccri.jur.pelitaperkebunan.v29i1.190

Cited by 4 publications

(5 citation statements)

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“…can occur through competition for space, nutrients, and oxygen, parasitism, and the ability to produce antibiotics or inhibitory compounds that are secreted into the growth media (Pasalo et al, 2022). According to Khairul et al (2017), Nur'Aini et al (2013 that Trichoderma hyphae are able to wrap around pathogenic hyphae so that the growth of pathogens is inhibited. Trichoderma sp.…”

Section: Resultsmentioning

confidence: 99%

“…This is in accordance with research (Haggag & El Soud, 2012) that antagonistic bacteria P. fluorescens are capable of producing antibiotics, such as antifungal, antibacterial, antitumor, and antiviral. Based on research (Nur'Aini et al, 2013) also said that the inhibition of the growth of C. gloeosporioides by P. fluorescens was caused by the presence of antibiotics such as agrocin, herbicolin, oomycin A, phenazine, pyoluteorin which are produced by Pseudomonas. Another mechanism suggested by P. fluorescens is by secreting a pseudobactin siderophore that is produced in a ferric ion-limited state.…”

Section: Resultsmentioning

confidence: 99%

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The Ability of Trichoderma sp and Pseoudomonas fluorencens to Inhibit the Growth of Anthracnose Fungus In Vitro at BBPP Ketindan Lawang

2024

Nusantara Science and Technology Proceedings

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Anthracnose is an important disease in chili plants because yield losses can reach 80%. This disease is caused by the fungus Colletotricum spp. The control that is often used is with chemical pesticides which cause negative effects on the environment. The refore it is necessary to use alternative control using biological agents that are environmentally friendly and support sustainable agriculture. This activity aims to determine directly the causes of anthracnose disease and the inhibition percentage of Tricoderma sp and Pseoudomonas fluorencens against anthracnose fungi in vitro on chili plants at the Ketindan Agricultural Training Center (BBPP). The test used the pour plate method (combining media with biological agents) with 3 treatments, 3 replications and 1 control. The test results showed that Trichoderma sp had an inhibition percentage of 100% while Pseudomonas fluorencens had an inhibition percentage of 28.06% and 100%. Trichoderma sp can inhibit the growth of anthracnose fungi because it has a competition mechanism for nutrients and space, hyper-parasitism, and produces antibiotic compounds while Pseoudomonas fluorencens has a mechanism for producing antibiotic compounds. Based on the observations, it can be concluded that Trichoderma sp and Pseoudomonas fluorencens were able to inhibit the growth of the fungus Colletotricum gloeospoioides in vitro.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The Ability of Trichoderma sp and Pseoudomonas fluorencens to Inhibit the Growth of Anthracnose Fungus In Vitro at BBPP Ketindan Lawang

2024

Nusantara Science and Technology Proceedings

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“…It was also reported by Djaenuddin et al (2014) that as many as 8 isolates of the best antagonist bacteria that had been identified, namely B. subtilis were able to inhibit the development of cob rot disease Fusarium moniliforme in maize in vitro and in vivo. The results of Aini et al (2013) stated that B. subtilis can produce macromolecular degradative enzymes that can destroy fungi cell walls such as proteases and several enzymes secreted in the medium such as amylase, glucanase, xylanase, chitinase, and proteases.…”

Section: Discussionmentioning

confidence: 99%

Isolation And Characterization of Bacteria from Shallots (Allium cepa L.) as In-vitro Biocontrol Agent of Fusarium oxysporum f.sp cepae

Radiastuti,

Amalia,

Suryadi

et al. 2024

Al-Kauniyah J. Biol.

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Shallot is one of the leading vegetable commodities with many benefits such as for seasonings and herbal medicinal ingredients. The demand for shallots continues to increase; however, shallot production is still relatively low. One of the limiting factors causing low shallot production is due to wilt disease caused by Fusarium oxysporum f.sp cepae (Foc). Bacteria have many roles in suppressing the growth of Foc, and this study aims to obtain potential bacterial isolates from the shallot plant to inhibit the growth of Foc Based on fungal diameter zone inhibition, degree of inhibition, and chitinase test, it was obtained 9 isolates which could suppress the growth of Foc. The results indicated that the AB3, TB2, and UB1 bacterial isolates could inhibit the growth of Foc with a percentage of inhibition of 46.80; 40.24; and 35.11%, respectively. The analysis showed that AB3, TB2, and UB1 isolates were categorized as moderate in suppressing the growth of Foc. The 16S rRNA sequencing results showed that AB3 and TB2 isolate had similarities with Bacillus subtilis by 99,75%, and 100%, respectively, while UB1 isolate had similarities with Pseudomonas nitroreducens by 89,35%. Based on the result showed that Bacillus sp. AB3 and TB2 isolates, and P. nitroreducens UB1 isolate have more potential as biological control agents to control the Fusarium wilt at in vitro assay. The field efficacy studies on these potential antagonists need to be done in the future.AbstrakSalah satu faktor pembatas yang menyebabkan rendahnya produksi bawang merah adalah penyakit layu yang disebabkan oleh Fusarium oxysporum f.sp cepae (Foc). Bakteri antagonis memiliki banyak peran dalam menekan pertumbuhan Foc, dan penelitian ini bertujuan untuk mendapatkan isolat bakteri antagonis potensial asal tanaman bawang merah untuk menghambat pertumbuhan Foc. Berdasarkan nilai zona hambat diameter jamur, derajat hambat dan uji kitinase, diperoleh 9 isolat yang dapat menekan pertumbuhan Foc. Hasil penelitian menunjukkan bahwa isolat bakteri AB3, TB2, dan UB1 mampu menghambat pertumbuhan Foc dengan persentase penghambatan masing-masing sebesar 46,80; 40,24; dan 35,11% dengan kategori penghambatan pertumbuhan Foc moderat. Hasil sekuensing 16S rRNA, menunjukkan bahwa isolat AB3 dan TB2 memiliki kemiripan dengan Bacillus subtilis masing-masing sebesar 99,75%, dan 100%, sedangkan isolat UB1 memiliki kemiripan dengan Pseudomonas nitroreducens sebesar 89,35%. Berdasarkan hasil penelitian menunjukkan bahwa Bacillus sp. isolat AB3, TB2, dan P.nitroreducens isolat UB1 berpotensi digunakan sebagai agen pengendali hayati untuk mengendalikan penyakit layu Fusarium pada uji in vitro. Studi kemanjuran lapangan terhadap isolat antagonis potensial ini perlu dilakukan di masa depan.

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“…Bacillus subtilis C10 was recognized as the isolate T2S1 with a 99.77% similarity, as shown in Figure 3. Gram-positive Bacillus subtilis possesses rodshaped cells that are 0.3-2.2 x 1.2-7.0 m in size [21]. By suppressing the growth of dangerous fungi, B. subtilis can produce antifungal substances such as subtilin, bacitracin, bacilli, and bacillomyxin [22].…”

Section: Identification Of Superior Chitinolytic Bacteriamentioning

confidence: 99%

Isolation of Chitinolytic Bacteria as Biocontrol Agent for Pathogenic Fungi on Cocoa Fruit in Sumbermanjing District, Malang

Sulistyantini

Mustafa²,

Jatmiko³

2023

JELS

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The productivity of cocoa plants in Sumbermanjing District is lower than in other parts of Malang Regency. Diseases like pathogenic fungi are to blame for the low output of cocoa plants. This research aimed to find chitinolytic bacteria that were superior at degrading chitin and limiting the growth of pathogenic fungi. These bacteria were isolated from cocoa garden soil and shrimp shell dumps. The bacteria from soil samples taken from the shrimp shell dump in Ujungpangkah District and the cocoa gardens in Sumbermanjing District were cultivated on an agar medium supplemented with colloidal chitin. The pathogenic fungi were discovered from decaying cocoa pods from Sumbermanjing District cocoa plantations. An antagonist test was used to gauge the inhibitory power, and a semi-quantitative test was used to measure the chitinolytic index. The similarity of 16S rDNA sequences allowed for the identification of superior bacterial isolates. The T2S1 isolates of chitinolytic bacteria had the highest levels of pathogenic fungi inhibition (25.14%, 20.85%, and 25.24%). With a 16S rDNA sequence similarity of 99.77%, Bacillus subtilis C10 was identified as the bacterium T2S1.

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Growth Inhibition of Colletotrichum gloeosporioides by Trichoderma harzianum, Trichoderma koningii, Bacillus subtilis and Pseudomonas fluorescens

Cited by 4 publications

References 0 publications

The Ability of Trichoderma sp and Pseoudomonas fluorencens to Inhibit the Growth of Anthracnose Fungus In Vitro at BBPP Ketindan Lawang

The Ability of Trichoderma sp and Pseoudomonas fluorencens to Inhibit the Growth of Anthracnose Fungus In Vitro at BBPP Ketindan Lawang

Isolation And Characterization of Bacteria from Shallots (Allium cepa L.) as In-vitro Biocontrol Agent of Fusarium oxysporum f.sp cepae

Isolation of Chitinolytic Bacteria as Biocontrol Agent for Pathogenic Fungi on Cocoa Fruit in Sumbermanjing District, Malang

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