Growth inhibition of human lens epithelial cells by short hairpin RNA in transcription factor forkhead box E3 (FOXE3)

Abstract

shRNA-mediated gene silencing of FOXE3 could significantly inhibit cell growth and induce the G1-phase arrest in HLEB-3 cells. Formation of posterior capsular opacification might be repressed if lens epithelial cell growth ceases after the FOXE3 gene is silenced with molecular biology technology.

FOXE3 contributes to Peters anomaly through transcriptional regulation of an autophagy-associated protein termed DNAJB1

“…We repeated the luciferase reporter assay in HLE cells to reproduce the results in a physiologically relevant system. The expression of wild-type FOXE3 caused significant alteration in shape and eventually death in HLE cells, consistent with previously published reports of ectopic expression of Foxe3 in the mouse lens 20 21 .…”

“…However, Wang et al . 20 previously reported that short-hairpin RNA (shRNA)-mediated silencing of FOXE3 significantly inhibits cell growth and induces G1-phase arrest in cultured HLE cells. Therefore, to preclude the possibility of confounding experimental interpretation, we opted to use cell lines negative for endogenous FOXE3 expression.…”

“…Previously, Wang et al . 20 reported that shRNA-mediated knockdown of FOXE3 in HLE cells resulted in an increased G0 population. Taken together, these results confirm that DNAJB1 is a downstream target of FOXE3.…”

MicroRNA-204-5p-Mediated Regulation of SIRT1 Contributes to the Delay of Epithelial Cell Cycle Traversal in Diabetic Corneas

“…The cells were treated with miR-204-5p antagomir, and the MTT assay was used to determine relative cell growth as described previously. 21 Data from the MTT colorimetric assay are shown in Figure 4. The absorbances were 0.940 6 0.028, 0.841 6 0.067, 0.258 6 0.051, 0.412 6 0.032, and 0.845 6 0.008 in the NG, HM, HG, HG plus miR-204-5p antagomir NTC, and HG plus miR-204-5p antagomir groups, respectively (n ¼ 3 for each group).…”