Growth inhibition of U937 leukemia cells by aqueous extract of Cordyceps militaris through induction of apoptosis

Park, Cheol; Hong, Su‐Hyun; Lee, Jae Yun; Kim, Gi‐Young; Choi, Byung Tae; Lee, Yong Tea; Park, Dong Il; Park, Yeong‐Min; Jeong, Yong-Kee; Choi, Yung Hyun

doi:10.3892/or.13.6.1211

Cited by 43 publications

(37 citation statements)

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“…Cordyceps militaris, an entomopathogenic fungus belonging to the class Ascomycetes, has been reported to have beneficial biological activities such as hypoglycemic, anti-inflammatory, anti-tumor, anti-metastatic, hypolipidemic, immunomodulatory, and antioxidant effects [2][3][4][5][6][7][8]. The fruiting bodies of wild C. militaris are expensive because of host specificity and rarity in nature.…”

Section: Introductionmentioning

confidence: 99%

Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture

Kwon

Lee

Shin

et al. 2009

Biotechnol Bioproc E

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Both crude exo-biopolymers and mycelial biomass, produced by liquid culture of `çêÇóÅÉéë species, are believed to possess several potential health benefits. As a result of its known biological activities, `çêÇóÅÉéë=ãáäáí~êáë has been extensively characterized in regards to potential medicinal applications. However, optimized liquid culture conditions for enhanced polysaccharide productivity have yet to be developed, which is a necessary step for industrial applications. Therefore, in this study, the liquid culture conditions were optimized for maximal production of mycelial biomass and exo-polysaccharide (EPS) by `K=ãáäáí~êáë. The effects of medium composition, environmental factors, and C/N ratio were investigated. Among these variables 80 g, glucose; 10 g, yeast extract; 0.5 g, MgSO 4 •7H 2 O; and 0.5 g, KH 2 PO 4 in 1 L distilled water were found to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, agitation, and aeration were determined to be 24 o C, uncontrolled pH, 200 rpm, and 1.5 vvm, respectively. Under these optimal conditions, mycelial growth in shake flask cultures and 5 L jar bioreactors was 29.43 and 40.60 g/L, respectively, and polysaccharide production in shake flask cultures and 5 L jar bioreactors was 2.53 and 6.74 g/L, respectively. © KSBB hÉóïçêÇëW=Cordyceps=militaris, ÉñçJéçäóë~ÅÅÜ~êáÇÉI=ãóÅÉäá~ä=ÖêçïíÜI=Å~êÄçåJíçJåáíêçÖÉå=ê~íáç= = = = =

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Section: Introductionmentioning

confidence: 99%

Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture

Kwon

Lee

Shin

et al. 2009

Biotechnol Bioproc E

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“…More recently, many studies have shown that the extracts of C. militaris or the bioactive metabolite cordycepin may induce apoptosis in various cell lines such as human colorectal cancer cell lines (SW480 and SW620), breast cancer cell line MDA-MB-231, leukemia (U937 and THP-1), neuroblastoma SK-N-BE(2)-C (CRL-2268), and melanoma SK-Mel-2 (HTB-68) cell lines (Park et al, 2005;Lee et al, 2006;Lui et al, 2007;Wu et al, 2007;Paterson, 2008;Tuli et al, 2013). Apoptosis is characterized mainly by a series of distinct changes in cell morphology, such as blebbing, loss of cell attachment, cytoplasmic contraction, DNA fragmentation, and other biochemical changes including the activation of caspases through extrinsic and/ or intrinsic mitochondrial pathways (Tuli et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Apoptotic effect of cordycepin on A549 human lung cancer cell line

Tuli¹,

Kumar²,

Sandhu³

et al. 2015

Turk J Biol

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In the Western world, lung cancer is still a leading cause of mortality, responsible for 170,000 deaths per year, more than deaths due to breast, colon, and prostate cancer combined (Danesi et al., 2003). Approximately 80%-85% of all lung cancers are classified as nonsmallcell lung cancer (NSCLC). It is very difficult to cure NSCLC, even in the early stage, as it develops resistance against chemotherapeutic agents. Therefore, treatment of NSCLC remains elusive despite the availability of a variety of chemotherapeutic agents that exhibit sophisticated mechanisms of action (

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“…In a study, Park et al [40] observed that the aqueous extract of C. militaris (AECM) inhibited cell growth of human leukemia U937 cells by morphological change and apoptotic cell death such as formation of apoptotic bodies and DNA fragmentation. They also observed the down-regulation of antiapoptotic gene bcl-2 expression and proteolytic activation of caspase-3 in AECM-treated U937 cells.…”

Section: Activity Of Cordyceps Militarismentioning

confidence: 99%

Cordyceps Mushroom: A Potent Anticancer Nutraceutical

Khan¹,

Tania²,

Zhang³

et al. 2010

TONUTRAJ

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Abstract:The Cordyceps mushrooms have a long history as medicinal fungi. In Traditional Chinese Medicine, Cordyceps have been used to treat several conditions including cancers for thousand of years. Extracts from both mycelium and fruiting bodies of C. sinensis, C. militaris and other Cordyceps species showed significant anticancer activities by various mechanisms such as, modulating immune system and inducing cell apoptosis. Some polysaccharide components and cordycepin (3'-deoxyadenosine) have been isolated from C. sinensis and C. militaris, which acted as potent anticancer components. This review article aims to further elucidate the importance of Cordyceps mushrooms by summarizing the findings of some of the important research works concerning possible mechanism of anticancer activity of this mushroom.

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Growth inhibition of U937 leukemia cells by aqueous extract of Cordyceps militaris through induction of apoptosis

Cited by 43 publications

References 0 publications

Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture

Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture

Apoptotic effect of cordycepin on A549 human lung cancer cell line

Cordyceps Mushroom: A Potent Anticancer Nutraceutical

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