Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture

Abstract

Both crude exo-biopolymers and mycelial biomass, produced by liquid culture of `çêÇóÅÉéë species, are believed to possess several potential health benefits. As a result of its known biological activities, `çêÇóÅÉéë=ãáäáí~êáë has been extensively characterized in regards to potential medicinal applications. However, optimized liquid culture conditions for enhanced polysaccharide productivity have yet to be developed, which is a necessary step for industrial applications. Therefore, in this study, the liquid culture conditions were optimized for maximal production of mycelial biomass and exo-polysaccharide (EPS) by `K=ãáäáí~êáë. The effects of medium composition, environmental factors, and C/N ratio were investigated. Among these variables 80 g, glucose; 10 g, yeast extract; 0.5 g, MgSO 4 •7H 2 O; and 0.5 g, KH 2 PO 4 in 1 L distilled water were found to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, agitation, and aeration were determined to be 24 o C, uncontrolled pH, 200 rpm, and 1.5 vvm, respectively. Under these optimal conditions, mycelial growth in shake flask cultures and 5 L jar bioreactors was 29.43 and 40.60 g/L, respectively, and polysaccharide production in shake flask cultures and 5 L jar bioreactors was 2.53 and 6.74 g/L, respectively. © KSBB hÉóïçêÇëW=Cordyceps=militaris, ÉñçJéçäóë~ÅÅÜ~êáÇÉI=ãóÅÉäá~ä=ÖêçïíÜI=Å~êÄçåJíçJåáíêçÖÉå=ê~íáç= = = = =

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

“…The least mycelia yield was produced with fructose, xylose and sorbitol in strains 218, 339 and 340 respectively. The result from the study is consistent with other work that reported starch and mannose as being widely utilized by many basidiomycetes [10] [18]. Starch utilization is possible because some basidiomycetes synthesize effective amylolytic enzymes for hydrolysis of starch.…”

“…Therefore, it is not surprising that the strains studied produced high biomass with mannose. Similar response is seen in other basidiomycetes including Cordycceps militaris [18], Pleurotus tuber-regium [20], and Grifola frondosa [21]. Intermediate utilization of mannitol by strain 218 and other basidiomycetes is associated with substrate oxidation or dehydrogenase enzyme activity [22].…”

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

“…These optimization methods involve three major steps: performing statistically designed experiments, estimating coefficients in a mathematical model to predict the response and checking the adequacy of the model [11,12]. RSM has been widely used in the optimization of the fermentation process such as media components for the production of enzyme [13,14], exopolysaccharides [15], other metabolites [16][17][18], biomass [19], and for predicting the growth rate of Bacillus cereus [20]. RSM is an effective tool for optimizing the process [21] when many factors and interactions affect the desired response.…”

Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris

“…After 11 days of cultivation, the culture broth was centrifuged at 5000 rpm for 20 min. Precipitated mycelia were washed three times with distilled water, and then freeze-dried (Kwon, Lee, Shin, Lee, & Hong, 2009). Dialysis tubing cellulose membranes, DEAE cellulose, Sepharose CL-6B, standard dextrans, lipopolysaccharide (LPS, Escherichia coli 0111:B4), laminarin, curdlan, and Congo red were purchased from Sigma Chemical Co. (St. Louis, MO, USA).…”