Growth Inhibitory Effect of Chicken Egg Yolk Antibody (IgY) on<i>Escherichia coli</i>O157:H7

Sunwoo, Hoon H.; Lee, E.N.; Menninen, K; Suresh, Mavanur R.; Sim, J. S.

doi:10.1111/j.1365-2621.2002.tb10310.x

Cited by 70 publications

(70 citation statements)

References 47 publications

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“…Table 1 shows the serum IgG concentration in Leghorn chickens and Tsaiya ducks at the start of the trial and after the third immunization. After 3 immunizations (week 7 of the experiment), the serum IgG level in Leghorn layer hens and Tsaiya ducks were increased significantly, this outcome consistent experimental results obtained by Sunwoo et al (2002), who also indicated that the antibody titer peaks after 3 immunizations. …”

Section: Protein Quantification Purity Determination Of Igy and Titesupporting

confidence: 78%

IgY Production by Taiwan Native Tsaiya Ducks and White Leghorn Laying Hens

Li¹,

JennFa²,

Chen³

et al. 2014

JAS

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The aim of this study was conducted to investigate a method to production purify IgY and compare the capacity between Taiwan native Tsaiya ducks (layer type) and Leghorn laying hens. Ten Taiwan native Tsaiya ducks and 10 white Leghorn laying hens (20 wk-old) were injected with 250 μL of complete Freund's adjuvant containing bovine serum albumin (BSA, 200mg) during week 1 of the trial. The boost injection, during week 2 and 4 of the trial was comprised 250 μL of incomplete Freund's adjuvant containing BSA was conducted to promote IgY production. Eggs were collected for IgY purification from the week 5 of the trial. Yolk protein was first extracted using isopropanol or triton and followed by salting out with ammonium sulfate. After dialysis, gel filtration chromatography and affinity chromatography were used to purify the IgY antibody. Yolk proteins extracted by isopropanol or triton from duck eggs were 43% and 13%, and 61% and 16% from hen eggs, respectively. After salting out, the duck IgY had a purity of 53−57% with a titer of 26.2−29.9 unit/mg protein, while the hen IgY had a purity of 45−50% with 24−29.5 unit/mg protein. After purification by gel filtration chromatography, the purity of the duck IgY was increased to 89−90% with 44.24−50.62 folds purification and 415−451 unit/mg protein. The purity of the chicken hen IgY was increased to 85−87% with 29.85−30.38 folds purification and 240−277.6 unit/mg protein. After further purification by affinity chromatography, duck IgY purity was increased to 91−92% with 45−56.26 folds purification and 459−461 unit/mg protein; chicken hen IgY purity was increased up to 93−95% with 33.58−59.49 folds purification and 312−470 unit/mg protein. This procedure is promising for purification and production of the IgY antibody from Taiwan native Tsaiya ducks and/or white Leghorn laying hens, and both of them are good producer of IgY.

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Section: Protein Quantification Purity Determination Of Igy and Titesupporting

confidence: 78%

IgY Production by Taiwan Native Tsaiya Ducks and White Leghorn Laying Hens

Li¹,

JennFa²,

Chen³

et al. 2014

JAS

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“…The concentrations of total IgY and experimental conditions utilized in our growth inhibition assays were standardized in preliminary experiments, by modifications of previous reports (Sugita-Konishi et al, 1996;Lee et al, 2002;Sunwoo et al, 2002).…”

Section: Growth Inhibition Assaysmentioning

confidence: 99%

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

et al. 2007

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IgY is a chicken egg yolk antibody which has been used for treatment and prophylaxis of gastrointestinal infections. Our aim was to verify if IgY obtained from chickens immunized with EPEC O111, STEC O111 and STEC O157 is able to show in vitro reactivity and biological activity towards the three bacteria. IgY was obtained from eggs laid before and after immunization with each bacterium. The preparations of IgY anti-EPEC O111 and anti-STEC O111 shared high reactivity detected by ELISA and growth inhibition ability towards both bacteria EPEC O111 and STEC O111. Nevertheless, the preparation of IgY anti-STEC O157 showed high reactivity and growth inhibitory effect only towards the homologous strain. Our results showing in vitro biological activity of IgY reinforce its use as an alternative for the treatment or prophylaxis of E. coli infections and encourage the development of in vivo studies for a possible future human therapeutic use.

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“…Recently, the utilization of IgY antibodies from the eggs of chickens which were immunized with pathogens has been the focus of attention in immunotherapy and diagnosis since the birds can actively transfer immunoglobulin to the egg yolk at the same concentration levels as in mammals (Buchwald and Profski 2003;Sunwoo et al 2002). The use of chicken antibody on a large scale offers several advantages, such as low costs, large yield and scaleable production and storability.…”

Section: Introductionmentioning

confidence: 99%

Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

Guimarães¹,

Amaral²,

Rangel³

et al. 2009

Arch. Immunol. Ther. Exp.

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Introduction: The aim was to evaluate the ability of egg yolk antibody (IgY) in blocking Staphylococcus aureus growth in vitro. Materials and Methods: Specific IgY was produced by immunizing hens with formalin-killed S. aureus (ATCC 33593). Specific IgY against S. aureus was obtained from the yolks of their eggs with a carrageenan solution. IgY was identified by SDS-PAGE and Western blot and its activity against S. aureus was tested by ELISA. A growth inhibition assay and protein concentration determination were also conducted. Results: ELISA indicated that the IgY was specific to the antigen; this activity was confirmed by Western blotting. The growth of S. aureus was inhibited by the specific IgY at concentrations of 1-5 µg/ml The bacteriostatic function of IgY appeared to result possibly from the interaction of IgY with surface components of S. aureus. In vitro experiments showed that the immunoglobulin from egg yolk interfered with the culture growth of the S. aureus. Conclusion: These findings indicate that eggs from hens immunized with appropriate antigens are a potentially useful source of passive immunity.

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Growth Inhibitory Effect of Chicken Egg Yolk Antibody (IgY) onEscherichia coliO157:H7

Cited by 70 publications

References 47 publications

IgY Production by Taiwan Native Tsaiya Ducks and White Leghorn Laying Hens

IgY Production by Taiwan Native Tsaiya Ducks and White Leghorn Laying Hens

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

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