Growth-inhibitory effect of tamoxifen and quercetin and presence of type II estrogen binding sites in human laryngeal cancer cell lines and primary laryngeal tumors

Ferrandina, Gabriella; Almadori, Giovanni; Maggiano, Nicola; Lanza, Paola; Ferlini, Cristiano; Cattani, Paola; Piantelli, Mauro; Ranelletti, Franco O.

doi:10.1002/(sici)1097-0215(19980831)77:5<747::aid-ijc14>3.0.co;2-z

Cited by 51 publications

(18 citation statements)

References 15 publications

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“…Additionally, the first clinical studies have been undertaken [8,23,29]. Similar studies also were conducted on a possible therapy with a testosterone substitute or a block of the 5α-reductase [14].…”

Section: Discussionmentioning

confidence: 99%

Analysis of sex-hormone-receptor expression in laryngeal carcinoma

Hagedorn¹,

Nerlich

2002

European Archives of Oto-Rhino-Laryngology

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There are significant differences in how laryngeal cancer affects the two genders, with cases occuring predominantly in males. This has been speculated to result from the different susceptibilities of the tumor cells to steroid sex hormones. Since the sex hormone action is mediated by specific cellular receptors, several previous studies have analyzed the presence of these sex-hormone receptors. However, the data on the receptor status for androgen, estrogen and progesterone receptors in laryngeal carcinomas are controversial. Since some authors have suggested antiandrogen or antiestrogen therapy as an adjuvant treatment for laryngeal carcinoma, we performed a comprehensive study using immunomorphological and biochemical techniques on both in-vivo and in-vitro tumor cells in order to clarify whether major sex-hormone receptors are present or not. We performed an immunohistochemical analysis on 13 patients with laryngeal carcinomas, 8 males and 5 females, using monoclonal antibodies against androgen, estrogen and progesterone receptors. In addition, the presence of estrogen and progesterone receptors was tested biochemically using an enzyme immune assay (EIA). Furthermore, we analyzed immunohistochemically the expression of the hormone receptors in five keratinocyte cell lines originating from laryngeal carcinomas. In all of our tumor samples, as well as in the five tumor cell lines tested, we did not detect any specific immunohistochemical staining for androgen, estrogen and progesterone receptors in the laryngeal carcinoma cells when compared to the positive controls of breast and prostate cancer samples. Similarly, the biochemical analysis did not demonstrate any significant amount of receptor protein in the analyzed cases. In conclusion, the absence of male and female sex-hormone receptors strongly argues against laryngeal carcinomas being sex-hormone-dependent tumors. Therefore, we do not see any rational indication to use a specific antiandrogen or antiestrogen therapy for the adjuvant treatment of laryngeal carcinomas.

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Section: Discussionmentioning

confidence: 99%

Analysis of sex-hormone-receptor expression in laryngeal carcinoma

Hagedorn¹,

Nerlich

2002

European Archives of Oto-Rhino-Laryngology

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“…Quercetin has been shown to be inversely associated with mortality from coronary heart disease by inhibiting the expression of metalloproteinase 1 (MMP-1), thus inhibiting the disruption of atherosclerotic plaques and contributing to plaque stabilization [4,5]. Quercetin also exerts anti-proliferative effects in vitro on cancer cell lines of diverse lineages, including colonic cancer cells [6][7][8][9]. Recently, a number of studies have demonstrated that quercetin has anti-infl ammatory effect through down-regulating NF-kappa B pathway, regulating NO release, and alleviating oxidative damage in the tissue [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Quercetin inhibits LPS-induced delay in spontaneous apoptosis and activation of neutrophils

et al. 2005

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As one of the pro-inflammatory factors, LPS aggravates inflammation through priming neutrophils to synthesize/release cytotoxic contents and prolonging functional lifespan of neutrophils by delaying the spontaneous apoptosis. Thus, our data suggest to us that quercetin might decrease the susceptibility of neutrophils to pro-inflammatory factors (e. g. LPS), which could partially explain the anti-inflammatory mechanisms of quercetin.

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“…Kaempferol was shown to inhibit G2/M cell cycle progression in human melanoma and rat prostate cancer lines in vitro (Casagrande & Darbon, 2001;Wang et al, 2003). Quercetin treatment arrested U937 human leukemia cells and laryngeal cancer cell lines in the G2/M phase of the cell cycle in vitro (Ferrandina et al, 1998;Lee et al, 2006). The above studies used kaempferol and quercetin aglycones to treat the cells.…”

Section: Discussionmentioning

confidence: 99%