Quercetin inhibits LPS-induced delay in spontaneous apoptosis and activation of neutrophils

Liu, J.-J.; Song, Can; Yue, Yang; Duan, Chunyan; Yang, Jie; He, Tao; He, Yue

doi:10.1007/s00011-005-1385-2

Cited by 44 publications

(34 citation statements)

References 34 publications

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“…The difference in the proportion of apoptotic PMN was the greatest after PBS, while after MDP and LPS it was manifested with more gradual increase. Analogously, previous studies have shown that MDP and LPS affect apoptosis of bovine PMN in vitro (Liu et al, 2005;Rysanek et al, 2005) and in vivo Rysanek, 2000, 2001). The results of this study indicate that only MDP and LPS prolong the duration of an acute inflammation.…”

Section: Discussionsupporting

confidence: 66%

Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Langrova¹,

Sládek²,

Rysanek³

2008

Vet. Med. - Czech

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The aim of the study was to prove the effect of muramyldipeptide and lipopolysaccharide on the expression of CD14 and CD44 during an induced inflammatory response of the mammary gland and its resolution. The purpose was to clarify whether the CD14 and CD44 expression is controlled by the mechanisms of resolution. The CD44 had previously been judged to be an activation marker along with CD11b on polymorphonuclear leukocytes. The experimental inflammatory response was induced by muramyldipeptide and lipopolysaccharide, while phosphate buffered saline was used as a control. The course of the inflammatory response was monitored at four time points: 24 h and 48 h (initiation of inflammatory response), 72 h and 168 h (resolution of inflammatory response). The total number of cells was determined by a hemocytometer. Flow cytometry was used to determine differential counts of leukocytes, proportions of CD11b+ polymorphonuclear leukocytes, proportions of apoptotic and necrotic polymorphonuclear leukocytes, and proportions of CD14+ and CD44+ polymorphonuclear leukocytes. The proportion of CD11b+ polymorphonuclear leukocytes after induction of inflammation with muramyldipeptide was higher (P < 0.05) compared to that after induction by phosphate buffered saline, was highly significantly greater after lipopolysaccharide (P < 0.01), and remained at approximately the same level for the whole period of observation (168 h). A higher proportion of CD14+ polymorphonuclear leukocytes was observed 72 h after induction with phosphate buffered saline. A statistically highly significant lower proportion was observed after induction with muramyldipeptide (P < 0.01), and a statistically significant lower proportion was observed after induction with lipopolysaccharide (P < 0.05). Decrease in the proportion of CD14+ polymorphonuclear leukocytes followed. In the initial phase of the inflammatory response (24 to 72 h) there was a gradual increase in the proportion of CD44+ polymorphonuclear leukocytes, and more so after the phosphate buffered saline. A greatly lower proportion of CD44+ polymorphonuclear leukocytes was observed after administration of muramyldipeptide and lipopolysaccharide: 24 h (P < 0.01), 48 h (P < 0.05) and 72 h (P < 0.01). Compared with muramyldipeptide and lipopolysaccharide, there was a statistically highly significant (P < 0.01) lower proportion of CD44+ polymorphonuclear leukocytes observed 168 h after induction with phosphate buffered saline. Hence the proportion of CD44+ polymorphonuclear leukocytes is low in the initial phase of inflammation, and CD44, in contrast with CD11b, does not appear to be a polymorphonuclear marker of activation. The results of the study have shown that expression of CD14 and CD44 is controlled by the factors inducing inflammatory response as well as by the mechanisms of resolution.

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Section: Discussionsupporting

confidence: 66%

Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Langrova¹,

Sládek²,

Rysanek³

2008

Vet. Med. - Czech

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“…One limitation, however, is that neutrophil priming is examined in isolated systems of purified agonist and chemically defined buffer solutions, instead of the complex, pathophysiologic mi-croenvironment presented in vivo. Indeed, although in vitro studies imply the relative stability of primed neutrophils in suspension [10,12,13], in vivo studies examining the priming behavior of circulating neutrophils indicate the highly interactive and proadherent nature of this cellular state [14 -17]. These studies indicate that priming facilitates conversion of circulating neutrophils to a proadherent state.…”

Section: Introductionmentioning

confidence: 99%

Reorganization of the human neutrophil plasma membrane is associated with functional priming: implications for neutrophil preparations

Stie

Jesaitis

2006

Journal of Leukocyte Biology

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Changes in the functional and plasma membrane organizational states of human neutrophils were examined using two isolation procedures, which may simulate altered physiological states in vivo. A gelatin-based method of blood-neutrophil isolation was used to model in vivo priming, and neutrophils isolated by this method were compared with control populations prepared by a pyrogen-free, dextran-based method. Gelatin-prepared neutrophils were functionally primed for adherence and agoniststimulated superoxide generation relative to unprimed, control neutrophils. The organizational state of the membrane cortex was examined by mapping the subcellular distribution of select cortical and transmembrane proteins by several methods, including subcellular fractionation, indirect immunofluorescence, and compositional analysis of Triton X-100-insoluble membrane skeleton preparations. Filamentous actin, fodrin, and the fodrin anchor, CD45, were largely cytoplasmic in unprimed neutrophils but translocated to plasma membranes upon priming, whereas CD43 and ezrin were exclusively surface-associated in both populations. Isopycnic sucrose density gradient analysis of N 2 -cavitated neutrophils revealed a major shift in the distribution of surface-associated transmembrane and membrane cortical components relative to the plasma membrane marker alkaline phosphatase in primed but not unprimed neutrophils. Similar results were obtained after neutrophil stimulation with known priming agents, LPS, TNF-␣, or GM-CSF. Together, these results may suggest that priming of suspended, circulating neutrophils is associated with a large-scale reorganization of the plasma membrane and associated membrane cortex in a process that is independent of cellular adhesion and gross morphologic polarization. J. Leukoc. Biol. 81: 672-685; 2007.

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“…For example, observational studies have reported that the 25 consumption of processed meats, relative to unprocessed meats, is associated with elevated 26 C-reactive protein (CRP) levels [4], risk of type II diabetes [4,5] and risk of cardiovascular 27 disease [6,7]. However, the specific components of these diets which are responsible for 28 promoting inflammatory signalling, or metabolic risk, remain to be clearly defined. 29 Recent evidence suggests that conserved molecules of microbial origin termed pathogen-30 associated molecular patterns (PAMPs) may play a key role in this relationship [8,9].…”

mentioning

confidence: 99%

“…Quercetin was also shown to inhibit LPS-induced CD11b activation 236 in human neutrophils in vitro [28]. The notion that processed onions promote inflammation 237 may therefore appear counter-intuitive.…”

mentioning

confidence: 99%

Reduced dietary intake of pro-inflammatory Toll-like receptor stimulants favourably modifies markers of cardiometabolic risk in healthy men

Herieka

Faraj

Erridge

2016

Nutrition, Metabolism and Cardiovascular Diseases

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Quercetin inhibits LPS-induced delay in spontaneous apoptosis and activation of neutrophils

Cited by 44 publications

References 34 publications

Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Reorganization of the human neutrophil plasma membrane is associated with functional priming: implications for neutrophil preparations

Reduced dietary intake of pro-inflammatory Toll-like receptor stimulants favourably modifies markers of cardiometabolic risk in healthy men

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