Growth kinetics of candida biofilm on medical polymers: A long‐term in vitro study

Leonhard, Matthias; Tobudic, Selma; Moser, Doris; Zatorska, Beata; Bigenzahn, Wolfgang; Schneider‐Stickler, Berit

doi:10.1002/lary.23662

Cited by 17 publications

(14 citation statements)

References 18 publications

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“…Our measurements fell within this range so it seems likely that C. albicans hyphae can penetrate host cell membranes using mechanical force alone. Consistent with this, C. albicans hyphae growing in biofilms are known to penetrate soft medical silicone, which has a Young's modulus similar to that of human cartilage (Leonhard et al ., 2013 ). The mechanical piercing of the murine oocyte with a needle distended the plasma membrane by 45 μm before it was finally breached (Sun et al ., 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Contact-induced apical asymmetry drives the thigmotropic responses ofCandida albicanshyphae

et al. 2014

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Filamentous hyphae of the human pathogen, Candida albicans, invade mucosal layers and medical silicones. In vitro, hyphal tips reorient thigmotropically on contact with small obstacles. It is not known how surface topography is sensed but hyphae lacking the cortical marker, Rsr1/Bud1, are unresponsive. We show that, on surfaces, the morphology of hyphal tips and the position of internal polarity protein complexes are asymmetrically skewed towards the substratum and biased towards the softer of two surfaces. In nano-fabricated chambers, the Spitzenkörper (Spk) responded to touch by translocating across the apex towards the point of contact, where its stable maintenance correlated with contour-following growth. In the rsr1Δ mutant, the position of the Spk meandered and these responses were attenuated. Perpendicular collision caused lateral Spk oscillation within the tip until after establishment of a new growth axis, suggesting Spk position does not predict the direction of growth in C. albicans. Acute tip reorientation occurred only in cells where forward growth was countered by hyphal friction sufficient to generate a tip force of ∼ 8.7 μN (1.2 MPa), more than that required to penetrate host cell membranes. These findings suggest mechanisms through which the organization of hyphal tip growth in C. albicans facilitates the probing, penetration and invasion of host tissue.

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Section: Discussionmentioning

confidence: 99%

Contact-induced apical asymmetry drives the thigmotropic responses ofCandida albicanshyphae

et al. 2014

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“…The main focus of previous reports was on testing short‐term biofilm on silicone using traditional staining methods, which is different from the biofilm on silicone‐based voice prostheses in patient over a long‐term period. In our previous study, an in vitro model for generating permanent mixed biofilm on medical polymers over a long‐term period and assessing biofilm coverage with a noninvasive analysis software was developed . The present study uses the similar method to grow the mixed species biofilms of C. albicans, C. tropicalis, S. salivarius, R. dentocariosa, S. epidermidis, and L. gasseri on medical‐grade silicone surface and test the inhibition of CM‐chitosan on this mixed species biofilm formation, which can avoid any alteration of the biofilm formation process and quantify biofilm growth kinetics with continuous documentation.…”

Section: Discussionmentioning

confidence: 99%

Long‐term antibiofilm activity of carboxymethyl chitosan on mixed biofilm on silicone

et al. 2016

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“…[249][250][251][252] Despite these advantages, the www.advmat.de www.advancedsciencenews.com lining process itself is potentially lengthy and sophisticated, [249] and polyurethane has a greater vulnerability to biofilm formation compared to silicone. [253] This is largely due to the appearance of microcracks on the material surface when immersed in water. [254]…”

Section: Liningmentioning

confidence: 99%

Past, Present, and Future of Soft‐Tissue Prosthetics: Advanced Polymers and Advanced Manufacturing

et al. 2020

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past 30 years, [12,13] it is only the last decade that has seen its rapid development as part of the fourth industrial revolution. [14] This change in practice is evidenced by the significant fraction of recent literature describing advances in 3D printed prostheses, including technological advances [4,15-18] and clinical reports. [19,20] Further innovations involving collaborations between clinicians, academics, and industry, promise even greater capabilities. The future will see 3D printers that can mix materials as desired during fabrication [21] and those that can 3D print organic semiconductors and piezoelectric polymers for sensing and bionics [22-24] (Figure 1). This report is focused on polymers for soft tissue, external, personalized, prosthetics with clinical applications for the ear, [25] nose, [26] eye, [27] face, [28] breast, [29] and hand. [30] The characteristics of both traditional and 3D printable polymeric materials, as well as current advanced manufacturing technologies and those in development, are also reviewed. The earliest evidence of prosthetics dates back to ≈2300 BC, where Egyptian mummies have been discovered with eye, nose, and genital reconstructions fashioned from plaster packed with mud, sand, linen, butter, or soda (Figure 1). [31] Wood, cloth, natural waxes, resins, and metals were later used as the material of choice for rudimentary prostheses. [32] Prostheses remained relatively unchanged for thousands of years until the 16th century, where prosthetic noses and eyes were made from parchment, wax, wood, hard rubber, gold, silver, and copper. [33] Metals continued as a fundamental material throughout the 19th century, largely due to their ability to be shaped and molded as needed. [34,35] One of the first polymers to be used in prosthetics, poly(methyl methacrylate) (PMMA), was developed in response to the glass shortages in the World Wars of the 20th Century. [36] This polymer went on to become the most common prosthetic material of the time, and still finds use today in artificial eyes [33] and prosthetic substructures. [37] Further innovations in the 20th century produced many new polymers, such as vinyls, copolymers, plastisols, and one of the most significant materials in prosthetics, silicone. [38] Discovered in the early 1900s by Fredrick Kipping, silicone was first used in prosthetics by George W Barnhart in 1960. [39] This versatile polymer remains a primary prosthetic material today due to its soft tissue-like properties, ease of manipulation, chemical inertness, durability, and excellent biocompatibility. [40,41] The search for alternative materials Millions of people worldwide experience disfigurement due to cancers, congenital defects, or trauma, leading to significant psychological, social, and economic disadvantage. Prosthetics aim to reduce their suffering by restoring aesthetics and function using synthetic materials that mimic the characteristics of native tissue. In the 1900s, natural materials used for thousands of years in prosthetics were replaced by syntheti...

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Growth kinetics of candida biofilm on medical polymers: A long‐term in vitro study

Abstract: The in vitro model presented in this study mimics in vivo events of biofilm formation on medical polymers with continuous monitoring of living biofilm kinetics.

Cited by 17 publications

References 18 publications

Contact-induced apical asymmetry drives the thigmotropic responses ofCandida albicanshyphae

Contact-induced apical asymmetry drives the thigmotropic responses ofCandida albicanshyphae

Long‐term antibiofilm activity of carboxymethyl chitosan on mixed biofilm on silicone

Past, Present, and Future of Soft‐Tissue Prosthetics: Advanced Polymers and Advanced Manufacturing

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