Growth of an enveloped mycoplasmavirus and establishment of a carrier state

Putzrath, Resha M.; Maniloff, Jack

doi:10.1128/jvi.22.2.308-314.1977

Cited by 43 publications

(37 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With this acholeplasma, 90% of the virus was bound in 30 min. Adsorption was first order, and the k constant was calculated to be 2.2 x 10-10 cm3/ min, which is identical to that reported by Putzrath and Maniloff (9). No adsorption of MVL2 by A. oculi or A. laidlawii strain JAlr occurred even after 60 min, which is indicative of the specificity of the receptor sites.…”

Section: Resultssupporting

confidence: 86%

Interaction of mycoplasma virus type 2 with cellular components of Acholeplasma laidlawii strain JA1

Al-Shammari¹,

Smith²

1980

J Virol

View full text Add to dashboard Cite

Mycoplasma virus type 2 was shown to adsorb specifically to intact cells, membranes, and lipoglycan of Acholeplasma laidlawii strain JA1 but not to these components of Acholeplasma oculi. The oligosaccharide chain of the lipoglycan defined the specificity of the receptor site since deacylation not only did not reduce adsorption but increased it threefold. Actual adsorption of virus to lipoglycan was demonstrated by sucrose density gradient separation of the virus-lipoglycan complex. A strain of A. laidlawii, JA1r, resistant to infection with mycoplasma virus type 2, was incapable of adsorbing the virus and was devoid of lipoglycan.

show abstract

Section: Resultssupporting

confidence: 86%

Interaction of mycoplasma virus type 2 with cellular components of Acholeplasma laidlawii strain JA1

Al-Shammari¹,

Smith²

1980

J Virol

View full text Add to dashboard Cite

show abstract

“…Early exponential phase A. laidlawii JAl cells (absorbance at 640 nm = 0.07) grown in tryptose broth were infected with MVL2 at a multiplicity of infection of about 1. The growth curve of the virus was very similar to that described previously (23). After a latent period of approximately 60 min, progeny virus was released.…”

Section: Resultssupporting

confidence: 76%

“…This association may suggest that the cell membrane is the site of assembly of intact MVL2 particles, but it is as yet unclear what the significance of the association of these specific polypeptides is. The modification of A. laidlawii membranes during infection, resulting in an increased resistance to lysis, was described recently (23). The possibility that the increased osmotic stability is due to the association of virus-induced polypeptides with A. Iaidlawii membranes cannot be excluded and merits further study.…”

Section: Discussionmentioning

confidence: 98%

Composition and molecular organization of lipids and proteins in the envelope of mycoplasmavirus MVL2

Greenberg¹,

Rottem²

1979

J Virol

View full text Add to dashboard Cite

MVL2 virus was purified from culture supernatants of infected Acholeplasma laidlawii cells by differential centrifugation, followed by velocity centrifugation in sucrose gradients. The purified virus contained 0.08 to 0.1 ,tmol of lipid phosphorous per ml of viral protein. Thin-layer chromatography of viral lipids revealed the presence of phospho-, glyco-, and phosphoglycolipids identical with those found in the host cell membrane, but the relative amount of phosphatidylglycerol was much lower than that in the virus. The fatty acid compositition of the viral lipids was, however, the same as that of the host membrane lipids. The lipids incorporated into the virus included lipids synthesized before and after infection. The freedom of motion of spin-labeled fatty acids in MVL2 depended markedly on temperature and on the position of the nitroxide group on the hydrocarbon chain of the probe, suggesting that the local environment of the probe has the properties of a lipid bilayer. Nevertheless, the lipid hydrocarbon chains in MVL2 appear to be less mobile than those in membranes of the host cells. Polyacrylamide gel electrophoresis of purified MVL2 revealed four major and about five minor polypeptide bands. None of the polypeptide bands gave a positive periodic acid-Schiff reaction. Lactoperoxidase-mediated iodination, followed by proteolytic digestion of intact MVL2 particles, revealed that at least two major polypeptides are localized on the external surface of the viral envelope. Acholeplasma laidlawii harbors three distinct virus groups, designated MVL1, MVL2, and MVL3 (10, 11, 16, 19, 20). MVL2 was found to be sensitive to detergents and organic solvents, whereas MVL1 and MVL3 were not (12, 19). Electron microscopy of sectioned MVL2 preparations showed the spherical virus particles to be bounded by a membrane-like structure (12). This observation, when combined with sensitivity to detergents, was taken to suggest that MVL2 is an enveloped, lipid-containing virus. The aim of the present study was to characterize the components of the MVL2 envelope and to compare its composition and molecular organization with those of the cell membrane of the host. 0.5 ml of an overnight culture of A. laidlawii and spread over 100-mm-diameter tryptose agar plates. The plates were allowed to dry and then placed in a 37°C incubator. Plaques were counted after 24 h of incubation. 717

show abstract

“…It was evident that although the number, relative amounts, and molecular weights of membrane proteins as judged from SDS-polyacryl-amide gel electrophoresis of total membrane proteins were very similar, there were considerable differences in the CIE patterns for the Tween 20-soluble proteins between A. laidlawii strains. It was also evident that membrane proteins from persistently infected JA1(2R) and JA1(3R) cells, which released limited amounts of virus corresponding to approximately 1 PFU/ 10i cells (33,34), behaved as if they were modified or associated with different boundary lipids (10).…”

mentioning

confidence: 99%

Membrane composition and virus susceptibility of Acholeplasma laidlawii

Steinick¹,

Wieslander²,

Johansson³

et al. 1980

J Bacteriol

View full text Add to dashboard Cite

The membrane composition of 11 strains ofAcholeplasma laidlawii, including three strains persistently infected with mycoplasmaviruses MVL51, MVL2, and MVL3, was studied and correlated with mycoplasmavirus sensitivity. Membranes of the strains had similar sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns, and all strains were inhibited by an antiserum produced against membranes from one of the strains. The amounts of integral membrane proteins solubilized by the nonionic detergent Tween 20 differed considerably. Therefore, characteristic crossed immunoelectrophoresis patterns were obtained for each strain. Strains persistently infected with MVL2 and MVL3 were notably different from the noninfected host. The ability to propagate any of the viruses was not correlated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis or crossed immunoelectrophoresis patterns. The persistently infected strains had a characteristic lipid composition. MVL51-resistant strains, including a resistant clone selected from a sensitive strain, were characterized by a large monoglucosyldiglyceride/diglucosyldiglyceride ratio and trace amounts of diphosphatidylglycerol (as opposed to the sensitive strains). Differences in lipid composition in A. laidlawii seem to affect the relationship between cells and viruses.

show abstract

Growth of an enveloped mycoplasmavirus and establishment of a carrier state

Cited by 43 publications

References 13 publications

Interaction of mycoplasma virus type 2 with cellular components of Acholeplasma laidlawii strain JA1

Interaction of mycoplasma virus type 2 with cellular components of Acholeplasma laidlawii strain JA1

Composition and molecular organization of lipids and proteins in the envelope of mycoplasmavirus MVL2

Membrane composition and virus susceptibility of Acholeplasma laidlawii

Contact Info

Product

Resources

About