Growth of Bacillus fastidiosus on glycerol and the enzymes of ammonia assimilation

Novel nitrogen sources for growth of Bacillus fastidiosus and their effect on the activity of NADP-dependent glutamate dehydrogenase

“…6.3.1.12) and glutamate synthase (E.C. 2.6.1.53) activities could not be demonstrated in cell-free extracts of B. fastidiosus [5].…”

“…fastidiosus it was shown that NADP-GDH activity was influenced by allantoin [5]. In this paper we report on three additional nitrogen sources for B.…”

“…Bacillus fastidiosus (DSM 83) was grown at 37°C with rotary shaking (200 rpm) in a minimal salts medium [5] supplemented with a carbon and a nitrogen source. To test for possible carbon sources which support growth, the minimal salts medium was supplemented with 2 mM allantoin and 10 mM of the carbon source, and when indicated 0.1% (w/v) BHI was added.…”

“…Cells were harvested at the end of the exponential phase and washed with 50 mM NazHPOa/KH2PO4 buffer (pH 7.0) containing 2 mM dithiothreitol. Cell-free extracts were prepared as described previously [5]. The protein content was determined by the Coomassie bril-liant blue G-250 method [8] using bovine serum albumin as the standard.…”

“…All attempts * Corresponding author. Tel: (080) 652940; Fax: (080) 553450. to grow the organism on other carbon and nitrogen sources, including tricarboxylic acid cycle intermediates, sugars, alcohols, amino acids, ammonium, urea and nitrate have failed [1][2][3][4][5]. Moreover, growth on complex media such as tryptone soy broth, nutrient broth and BHI (all tested at 3%, w/v), was very limited or absent [3].…”