Growth of Cells in Agitated Fluid Medium

Cultivation of L Strain (Earle) Mouse Cells in Bacteriological Media Combined with Horse Serum

“…The medium reported here supported the growth of Gey's MBIJI strain (mouse lymphoblasts) ( 19), Maben strain (a human epithelium-like cell derived from an adenocarcinoma of Ithe lung)(20), and a strain of guinea pig lung fibroblasts that wits isolated in this laboratory. Under these conditions, the yeast extract-proteose peptone #3 combination in the presence of 20% horse serum produced slightly more growth than dild the standard reference medium.…”

Cultural Characterization of Animal Cells

“…Whether giant cells, which may be multinucleate, differ in origin from smaller multinucleate cells, is not known. A possible variety of etiological factors is suggested by the description given by Phillips & Terryberry (291) of an L-cell substrain containing frequent giant cells, by an associ ation of frequency of giant cells with a reduction of magnesium ion in the medium observed by Owens et al (275 ), and by the inducing effect of bovine-serum albumin fraction on L cells reported by Mitamura (251). Variation in morphology of animal-cell colonies in the same dish was de scribed by Ackermann (4), for HeLa cells.…”

Training Hela and L Strain Cells to Grow in Fluid-Suspension

“…Among those works most cases were of mouse fibroblast L strain cells (Earle et al, 1954;Kuchler et al, 1956;Danes, 1957;and Siminovitch et al, 1957). Some others were of special cell types which had naturally gained a tendency to float out of glass surface during a continuous cultivation (Graham et al, 1955) or could reasonably be supposed to grow in suspension by their original characteristics : mouse lymphoblastoma cells reported by Owens et al (1954) and rat ascites hepatoma cells by Takaoka (1958). There are very few reports on the suspension culture of epithelial cells.…”

“…According to investigators, variously devised equipments were introduced such as tumbling tubes by Owens et al (1953Owens et al ( , 1954; rapid roller-tubes by Earle et al (1954), Graham et al (1955), Siminovitch et al (1957) and Takaoka (1958); a rotary shaker by Kuchler et al (1956); a magnetic stirrer by McLimans et al (1957); a glass stirrer by Danes (1957); and shaker flasks with aeration by Earle et al (1956) and Bryant et al (1958). While the successful production of massive population and steady growth rate in suspension were made with a few special cell types, the com paratively low growth rate, which was suppcsedly due to mechanical injuries, and the inconstant growth mode were the general trend with other types.…”