Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function

Käding, Nadja; Kaufhold, Inga; Müller, Constanze; Szaszák, Márta; Shima, Kensuke; Weinmaier, Thomas; Lomas, Rodrigo; Conesa, Ana; Schmitt‐Kopplin, Philippe; Rattei, Thomas; Rupp, Jan

doi:10.3389/fcimb.2017.00499

Cited by 19 publications

(17 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nutrient acquisition is then one of the most fundamental aspects of bacterial pathogenesis. Colonization allows the organism to have continual access to a nutrient source, while immunoevasion and immunosuppression mechanisms allow the pathogen to continue to access the host's nutrient supply [7], but host cell metabolism is also influenced by many intracellular bacterial pathogens, and the microbiome also plays a role in metabolic hostpathogen interaction [8,9]. Intracellular pathogens are diverse in the types of hosts they parasitize, the nutrients they utilize, and the host cellular processes they target.…”

mentioning

confidence: 99%

Nutrition and Bipartite Metabolism of Intracellular Pathogens

Best

Kwaik

2019

Trends in Microbiology

View full text Add to dashboard Cite

The host is a nutrient-rich niche for microbial pathogens, but one that comes with obstacles and challenges. Many intracellular pathogens like Legionella pneumophila, Coxiella burnetii, Listeria monocytogenes, and Chlamydia trachomatis have developed bipartite metabolism within their hosts. This style of metabolic regulation enables pathogen sensing of specific nutrients to engage them into catabolic and anabolic processes, and contributes to temporal and spatial pathogen phenotypic modulation. Not only have intracellular pathogens adapted their metabolism to the host, they have also acquired idiosyncratic strategies to exploit host nutritional supplies and intercept metabolites. Francisella tularensis and Anaplasma phagocytophilum alter host autophagy, Shigella flexneri intercepts all host pyruvate, while L. pneumophila induces host protein degradation and blocks protein translation. Strategies of pathogen manipulation of host nutrients could serve as therapeutic targets.

show abstract

mentioning

confidence: 99%

Nutrition and Bipartite Metabolism of Intracellular Pathogens

Best

Kwaik

2019

Trends in Microbiology

View full text Add to dashboard Cite

show abstract

“…Moreover, PC cell lines have been shown to have an impaired mitochondrial respiration rate and exemplify the Warburg effect [27, 30], resulting in enhanced glycolysis, lactogenesis, and inefficient ATP synthesis. However, as recently reported, moderate hypoxia is highly beneficial for the chlamydial growth cycle in cultured cells and promotes the infection rate of both C. trachomatis and C. pneumoniae [28]. Additionally, as shown lately [11] C. trachomatis can sustain its own energy needs during the infectious cycle at least in part via sodium-dependent synthesis of ATP.…”

Section: Discussionmentioning

confidence: 95%

“…CWR-R1 cells are known constitutively to express functional elements of mitogen-activated protein kinase, phosphatidylinositol-3 kinase, and Akt pathways and to display hyperactivated Akt which develops due to an Akt-dependent increase in oxidative phosphorylation [26, 27]. These changes are known to cause an intracellular excess of reactive oxygen species (ROS) which are an essential stimulus for initiation and progression of the developmental cycle of C. trachomatis in the urogenital system [3, 28]. It has been shown recently that high ROS production promotes chlamydial growth and infective progeny formation via activation of caspase-1 and hypoxia-inducible factor 1 α [29].…”

Section: Discussionmentioning

confidence: 99%

Chlamydia trachomatis Growth and Cytokine mRNA Response in a Prostate Cancer Cell Line

Petyaev

Zigangirova

Morgunova

et al. 2019

Advances in Urology

View full text Add to dashboard Cite

In the present paper, we report that C. trachomatis can be efficiently propagated and affect mRNA expression for two major cytokines, relevant to tumor progression, in CWR-R1 cells, a malignant prostate cell line. CWR-R1 and McCoy cells, a classic cell line for chlamydial research, were grown and infected with C. trachomatis under similar conditions. Cell monolayers were harvested for RNA analysis and immunostaining with major outer membrane protein (MOMP) antibody at 24, 48, and 72 hours of the postinfection (hpi) period. It was shown that the infectious cycle of chlamydial pathogen in CWR-R1 cells resembles the progression of C. trachomatis infection in McCoy cells but with a few important differences. First of all, the initial stage of C. trachomatis propagation in CWR-R1 cells (24 hpi) was characterized by larger inclusion bodies and more intense, specific immunofluorescent staining of infected cells as compared with McCoy cells. Moreover, there was a corresponding increase in infective progeny formation in CWR-R1 cells along with mRNA for EUO, a crucial gene controlling the early phase of the chlamydial development cycle (24 hpi). These changes were more minimal and became statistically insignificant at a later time point in the infectious cycle (48 hpi). Altogether, these data suggest that the early phase of C. trachomatis infection in CWR-R1 cells is accompanied by more efficient propagation of the pathogen as compared with the growth of C. trachomatis in McCoy cells. Furthermore, propagation of C. trachomatis in CWR-R1 cells leads to enhanced transcription of interleukin-6 and fibroblast growth factor-2, genes encoding two important proinflammatory cytokines implicated in the molecular mechanisms of chemoresistance of prostate cancer and its ability to metastasize. The possible roles of reactive oxygen species and impaired mitochondrial oxidation in the prostate cancer cell line are discussed as factors promoting the early stages of C. trachomatis growth in CWR-R1 cells.

show abstract

“…while Chlamydia pneumoniae infection increases monocyte glucose uptake (Rupp et al, 2007) and mitochondrial functions, favouring bacteria intracellular growth (Käding et al, 2017). It has been shown that under hypoxia, macrophage HIF-1α was stabilised, and the TCA metabolites availability was reduced.…”

Section: Intracellular Oxygen Level Decreases Upon Bacterial Infectionmentioning

confidence: 99%

The selective advantage of facultative anaerobes relies on their unique ability to cope with changing oxygen levels during infection

Andre

Debande

Marteyn

2021

Cellular Microbiology

View full text Add to dashboard Cite

Bacteria, including those that are pathogenic, have been generally classified according to their ability to survive and grow in the presence or absence of oxygen: aerobic and anaerobic bacteria, respectively. Strict aerobes require oxygen to grow (e.g., Neisseria), and strict anaerobes grow exclusively without, and do not survive oxygen exposure (e.g., Clostridia); aerotolerant bacteria (e.g., Lactobacilli) are insensitive to oxygen exposure. Facultative anaerobes (e.g., E. coli) have the unique ability to grow in the presence or in the absence of oxygen and are thus well-adapted to these changing conditions, which may constitute an underestimated selective advantage for infection. In the WHO antibiotic-resistant 'priority pathogens' list, facultative anaerobes are overrepresented (8 among 12 listed pathogens), consistent with clinical studies performed in populations particularly susceptible to infectious diseases.Bacteria aerobic respiratory chain plays a central role in oxygen consumption, leading to the formation of hypoxic infectious sites (infectious hypoxia). Facultative anaerobes have developed a wide diversity of aerotolerance and anaerotolerance strategies in vivo. However, at a single cell level, the modulation of the intracellular oxygen level in host infected cells remains elusive and will be discussed in this review. In conclusion, the ability of facultative bacteria to evolve in the presence or the absence of oxygen is essential for their virulence strategy and constitute a selective advantage. Take Away• Most life-threatening pathogenic bacteria are facultative anaerobes.• Only facultative anaerobes are aerotolerant, anaerotolerant and capable of consuming O 2 .• Facultative anaerobes induce and are well adapted to cellular hypoxia.

show abstract

Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function

Cited by 19 publications

References 49 publications

Nutrition and Bipartite Metabolism of Intracellular Pathogens

Nutrition and Bipartite Metabolism of Intracellular Pathogens

Chlamydia trachomatis Growth and Cytokine mRNA Response in a Prostate Cancer Cell Line

The selective advantage of facultative anaerobes relies on their unique ability to cope with changing oxygen levels during infection

Contact Info

Product

Resources

About