Growth of embryogenic sweet orange callus on media varying in the ratio of nitrate to ammonium nitrogen

Niedz, Randall P.

doi:10.1007/bf00037584

Cited by 32 publications

(13 citation statements)

References 17 publications

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“…Sodium is a trace element important for some plants (Salisbury and Ross 1992), but its role on the micropropagation process has not been evaluated. Finally, we can speculate that these variations in the mineral composition can interfere with membrane transport and affect the pH of plant cells, thus influencing the in vitro response (Niedz 1994). However, it should be noted that experiments performed with A. unedo and other Ericaceae indicate that different culture media, other cytokinins, and sucrose were also able to promote shoot proliferation.…”

Section: Discussionmentioning

confidence: 98%

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Gomes

Canhoto

2008

In Vitro Cell.Dev.Biol.-Plant

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Arbutus unedo L. is a species of strawberry tree, widely represented in the Mediterranean climates of southern Europe. Fruits are used to make jellies and a spirit called "medronheira." Shoot apices and nodal segments from epicormic and coppiced shoots of adult plants were used for plant propagation. Shoot apices from epicormic shoots, which were developed in a growth chamber, showed higher rates of in vitro establishment. The results also indicated that shoot apices are more effective for plant establishment than nodal segments, with rates of establishment significantly higher after 12 wk of culture. Of the three basal media used in combination with 9.0 µM benzyladenine and 0.087 M sucrose, the FS medium with the micronutrients of the Murashige and Skoog medium gave the highest rates of multiplication, especially when the parameter analyzed was the number of clusters formed. When shoot apices from selected adult plants (AL01-AL06) were tested, the multiplication rate was not significantly different among the plants. However, in the conditions tested, shoots from the clones AL1, AL2, and AL3 showed better development, whereas shoots from AL4, AL5, and AL6 showed an impaired development and could not be rooted. Rooting was achieved in all the conditions tested, even in the absence of auxin. The inclusion of an auxin significantly increased root formation, whereas the addition of charcoal did not improve root formation. Rooted plantlets were acclimatized, and some of them are now in the field for further study.

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Section: Discussionmentioning

confidence: 98%

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Gomes

Canhoto

2008

In Vitro Cell.Dev.Biol.-Plant

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“…The absorption of NO 3 -anions results in increased pH levels due to an excretion of HCO 3 -from the explant. Conversely, absorption of NH 4 + cations results in a release of H + reducing the pH of the medium (Niedz 1994). Thus, in the present work the latter negative effects of inorganic N sources were avoided by adding arginine, as organic nitrogen, to the culture medium.…”

Section: Effect Of Arginine On Induction Of Somatic Embryogenesismentioning

confidence: 95%

Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline

Nieves

Sagarra

Gonzalez

et al. 2008

Plant Cell Tiss Organ Cult

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The aim of this study was to evaluate the effect of arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and other nitrogenous compounds contents. Segments of leaves were used as explants to establish embryogenic cultures on media with 0 and 50.0 mg l -1 arginine. Somatic embryos formation and free polyamines, free proline and total soluble proteins contents were compared. Arginine significantly induced sugarcane somatic embryogenesis. Free proline and protein levels determined in embryogenic cell masses during embryo differentiation-maturation, showed an arginine-induced promotion associated to the enhancement of the embryogenic process. In addition, free putrescine and, in a minor extent, spermidine and spermine contents were enhanced by arginine.

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“…Studies with Pinus strobus L. revealed a total inhibition of callus growth if ammonium was the only nitrogen source available [42], and Oryza sativa L. suspension cultures showed inhibited growth if given ammonium [43]. Furthermore, Citrus sinensis (L.) Osbeck embryogenic callus growth was significantly reduced if the majority of inorganic nitrogen was supplied as ammonium [44]. Another study carried out with Oryza sativa cells found that L-glutamine supplementation had no positive effect on the internal ammonium content [45].…”

Section: Morphological and Developmental Characterization And Nitrogmentioning

confidence: 99%

Analysis of Nitrogen Utilization Capability during the Proliferation and Maturation Phases of Norway Spruce (Picea abies (L.) H.Karst.) Somatic Embryogenesis

Dahrendorf

Clapham

Egertsdotter

2018

Forests

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Somatic embryogenesis (SE) is a laboratory-based method that allows for cost-effective production of large numbers of clonal copies of plants, of particular interest for conifers where other clonal propagation methods are mostly unavailable. In this study, the effect of L-glutamine as an organic nitrogen source was evaluated for three contrasted media (containing NH 4 + NO 3 without glutamine, or glutamine + NO 3 , or glutamine without inorganic nitrogen) during proliferation and maturation of Norway spruce somatic embryos through analyses of activities of the key enzymes of nitrogen metabolism: nitrate reductase (NR), glutamine synthetase (GS) and arginase. A major change in nitrogen metabolism was indicated by the increased activity of GS from zero in the proliferation stage through maturation to high activity in somatic embryo-derived plantlets; furthermore, NR activity increased from zero at the proliferation stage to high activity in maturing embryos and somatic-embryo derived plantlets. In contrast, arginase activity was high at all stages. In accordance with the GS and NR data, proliferation was fastest on the glutamine media, the largest number of mature embryos developed on the glutamine medium without inorganic nitrogen, and the ammonium content of proliferating cultures was much higher than that of mature somatic embryos. The best developed embryos, judged by germination, were from the glutamine + NO 3 medium. These insights into nitrogen utilization will help to improve culture medium composition.

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Growth of embryogenic sweet orange callus on media varying in the ratio of nitrate to ammonium nitrogen

Cited by 32 publications

References 17 publications

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline

Analysis of Nitrogen Utilization Capability during the Proliferation and Maturation Phases of Norway Spruce (Picea abies (L.) H.Karst.) Somatic Embryogenesis

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