Growth of lactic acid bacteria on oilseed crop pea- and chickpea-based media

Abstract

Six strains of lactic acid bacteria isolated from different biotopes were cultivated on oilseed crop pea-or chickpea-based media, supplemented with the usual nitrogen supplements, yeast extract, meat extract or tryptic casein peptone. Growth was compared to that recorded on the widely used MRS and M17 media. All the media tested favoured growth of the selected strains, since the final c.f.u. gained at least 3 logs. Vegetal substrate (pea) appeared especially efficient for growth of a LAB species isolated from plants, Lactobacillus plantarum, illustrating the relationship between the supplementation used and the natural biotope of the LAB strain; during growth on oilseed crop pea-and chickpea-based media, the final c.f.u. was 0.58 and 1.28 log higher than the final cell numeration recorded on the reference medium MRS. The better efficiency of chickpea to stimulate LAB growth, if compared to oilseed crop pea, must be related to its higher protein content, 24.15 ± 0.1 and 28.2 ± 0.5% respectively. A vegetal substrate with high protein content can be therefore used to replace, at least partially, the usual nitrogen supplements.

Development of a sweet whey-based medium for culture of Lactobacillus

“…Rich and complex media, like MRS (De Man et al, 1960) or M17 (Terzaghi and Sandine, 1975) (Kurbanoglu, 2004), corn steep liquor (Cornelius et al, 1996;Marcela et al, 2016), whey protein hydrolysates (Mc Comas and Gilliland, 2003;Watanabe et al, 2004), malt sprout extract, casein hydrolysates (Vahvaselkä and Linko, 1987;Chiarini et al, 1992;Horáčková, 2014), baker's yeast cells (Altaf et al, 2007), vegetal substrates (Gardner et al, 2001;Nancib et al, 2001;Charalampopoulos et al, 2002;Djeghri-Hocine et al, 2006;Djeghri-Hocine et al, 2007a), sugarcane juice (Preeti et al, 2016), Palmyra palm jaggery (Reddy Tadi et al, 2017), and de-lipidated egg yolk (DjeghriHocine et al, 2007b). The most efficient nitrogen source seems to be yeast extract (Aeschlimann and Von Stokar, 1990;Arasaratnam et al, 1996;Göksungur and Gűvenç, 1997;Guha et al, 2013;Vethakanraj et al, 2013).…”

Evaluation of de‐lipidated egg yolk and yeast autolysate as growth supplements for lactic acid bacteria culture

“…2002; Djeghri‐Hocine et al . 2006; Djeghri‐Hocine et al . 2007) or fish viscera hydrolysates (Horn et al .…”

Cultivation media for lactic acid bacteria used in dairy products

“…The authors indicated that horse bean extract was a cost-effective medium for the growth of LAB species isolated from plants. Oilseed crop pea and chickpea were likewise used to replace expensive nitrogen sources in a LAB cultivation medium (Djeghri-Hocine et al ., 2007 b ). The developed medium was supplemented with small amounts of yeast extract, meat extract or tryptic casein peptone, 5 g/l.…”

“…For example, oilseed crop pea and chickpea-based media showed higher bacterial populations than MRS. These plants are rich in protein and thus can be used to partially replace the expensive nitrogen sources that are typically used in LAB media (Djeghri-Hocine et al ., 2007 b ). A combination of sieved wheat stillage and a sugar production byproduct, molasses, was suggested for the low cost, industrial production of Lb.…”