Growth of Neurospora crassa in Unstirred Liquid Cultures

Gillie, O. J.

doi:10.1099/00221287-51-2-179

Cited by 11 publications

(7 citation statements)

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“…While the generation times of most fungi in our phylogeny are unknown, the generation times of model organisms in Saccharomycotina are thought to be shorter than those in Pezizomycotina. For example, the doubling time of the budding yeasts S. cerevisiae and C. albicans under optimal conditions is 90 min (39), while that of the filamentous fungi Aspergillus nidulans and Neurospora crassa is between 2 and 3 hours (40,41). It is also well established that absence or loss of DNA repair genes can lead to an increase in genomes' mutation rates (42).…”

Section: Contrasting Modes Of Genome Evolution In Fungal Phylum Ascommentioning

confidence: 99%

Genome-scale phylogeny and contrasting modes of genome evolution in the fungal phylum Ascomycota

et al. 2020

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Ascomycota, the largest and most well-studied phylum of fungi, contains three subphyla: Saccharomycotina (budding yeasts), Pezizomycotina (filamentous fungi), and Taphrinomycotina (fission yeasts). Despite its importance, we lack a comprehensive genome-scale phylogeny or understanding of the similarities and differences in the mode of genome evolution within this phylum. By examining 1107 genomes from Saccharomycotina (332), Pezizomycotina (761), and Taphrinomycotina (14) species, we inferred a robust genome-wide phylogeny that resolves several contentious relationships and estimated that the Ascomycota last common ancestor likely originated in the Ediacaran period. Comparisons of genomic properties revealed that Saccharomycotina and Pezizomycotina differ greatly in their genome properties and enabled inference of the direction of evolutionary change. The Saccharomycotina typically have smaller genomes, lower guanine-cytosine contents, lower numbers of genes, and higher rates of molecular sequence evolution compared with Pezizomycotina. These results provide a robust evolutionary framework for understanding the diversity and ecological lifestyles of the largest fungal phylum.

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Section: Contrasting Modes Of Genome Evolution In Fungal Phylum Ascommentioning

confidence: 99%

Genome-scale phylogeny and contrasting modes of genome evolution in the fungal phylum Ascomycota

et al. 2020

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“…Although there are many conditions, such as nonoptimal temperature and genetic constitution, that will markedly diminish this rate (38,57), the rate of advance of a mycelial front on a solid medium usually depends surprisingly little on nutrition. It is the density of branching behind the front that varies dramatically (56,57). Hence, on a rich, mediocre, or poor medium, Neurospora can generally push forward a mycelial front at a rate of 10 to 14 cm/ day.…”

Section: Miscellaneous Underexploitedmentioning

confidence: 99%

Implications of some genetic control mechanisms in Neurospora.

Metzenberg¹

1979

Microbiological Reviews

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INEODUCTION Apologia For the would-be writer of a review article, it can be sobering to ask whether the article is needed. On posing this question to myself, I realized that a number of very perceptive and, in some cases, extensive reviews have already appeared in print. These are listed below. I saw few ways to cover the same ground differently, and certainly no way to cover it better. It seemed more profitable to use this space as an opportunity (i) to examine a few of the special features of Neurospora crassa and closely related species that are likely to escape attention elsewhere and (ii) for some unabashed speculation about the evolutionary pressures that have shaped the control mechanisms extant in Neurospora and perhaps in other eucaryotes. This article is meant to be read with that in mind. Books, Reviews, and Other Sources of Information The following sources will be useful to those wishing to get abreast of the literature of Neurospora. Fincham and Day (45). General fungal genetics textbook, with emphasis on Neurospora. Chromosome mechanics, mutagenesis, mapping functions, fine structure determination, extrachromosomal inheritance, physiological genetics. Davis (37). A critical review of metabolic organization and compartmentation of function in Neurospora and other fungi. Prevention of futile cycling by summation of anabolism and catabolism. Marzluf (89). A valuable discussion of genetic regulation of metabolic pathways in fungi. Perkins and Barry (103). A remarkable, comprehensive review of cytogenetics, meiosis and mitosis, and chromosomal aberrations; life cycle of N. crassa and related heterothallic and pseudohomothallic species. Contains genetic maps and tabulations of kinds of auxotrophs and morphological, color, and other kinds of mutants. Perkins et al. (105). Natural history of the genus Neurospora. Taxonomy and chromosome homology of a large collection of isolates from nature. Srb et a1. (121). Genetics of development of the sexual apparatus. Nelson et al. (98). Biochemical and morphological changes during conidium formation, especially as revealed by mutational dissection. Schmit and Brody (116). Biochemistry and physiology of conidial germination. Brody (20) and Scott (117). Effect of enzyme levels and metabolic pools on cell wall and membrane composition and on morphology. Mishra (95). Various aspects of morphogenesis. Bachmann and Strickland (12). An inval-361

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“…The inoculum doubling-time as calculated above is remarkably close to the inoculum doubling-time determined (by using the same reasoning) for growth in unstirred liquid cultures (Gillie, 1968) of 1.84 hr for Neurospora strain STA (95% confidence limits 1-74 to 1-94 hr) and in reasonable agreement with doubling times for strain STA, grown in shaken logarithmic cultures, of 2.3 hr (95 yo confidence limits I 07-2.9 hr) (C. F. Curtis, personal communication).…”

Section: Growth Of Neurospora In Tubes I 9mentioning

confidence: 50%

“…It was not found practical to measure the very low yields obtained at low growth rates and so the relationship between the two parameters was not explored below about I mg. mycelium/tube. The relationship between inoculum size and lag in growth In a previous paper (Gillie, 1968) the relationship between inoculum size and growth in exponential cultures of micro-organisms was considered and it was shown that when the inoculum size was varied in a series of cultures and the weight of each culture after growth considered as a constant value (in practice by interpolating between experimental values), then from the exponential growth equation log, M -loge Mo = pt (where Mo is mass of inoculum and M is the mass of the culture at time t), it can be seen that if M is treated as constant, -1OgeMO is proportional to t. A relationship of this type was found to hold between 'lag' (time after inoculation when growth first becomes measurable) and inoculum size for linear growth of Neurospora in tubes. The experimental investigation of this relationship is described below.…”

Section: Growth Of Neurospora In Tubes 189mentioning

confidence: 99%

“…Models of spherical growth of fungi in liquid culture (discussed by Gillie, 1968), which assume a constant rate of increase of the radius of the sphere, are based on the linear rate of increase of the radius of fungal colonies on solid media, and tend to assume that the density of the organism is uniform throughout the 'sphere of growth' considered. However, the results described here show that an increase in yield takes place behind the growing front to a maximum value, after which a decrease in yield occurs.…”

Section: Growth Of Neurospora In Tubes I 9mentioning

confidence: 99%

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Observations on the Tube Method of Measuring Growth Rate in Neurospora crassa

Gillie¹

1968

Journal of General Microbiology

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S U M M A R YThe relationship between various parameters of growth of Neurospora crassa on solid media in tubes is described. These are (a) the relationship between culture weight and linear growth rate on various media; (b) the relationship between 'lag' in growth and inoculum size on various media. This latter relationship appears to obey a log law, enabling an inoculum doublingtime to be calculated. Adaptation of the arginine auxotroph arg-I (46004) to diminution of arginine in the medium first occurred by decrease in mycelial weight and then by decrease in linear growth rate. The inoculum doublingtime did not appear to vary systematically with arginine concentration.

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Growth of Neurospora crassa in Unstirred Liquid Cultures

Cited by 11 publications

References 1 publication

Genome-scale phylogeny and contrasting modes of genome evolution in the fungal phylum Ascomycota

Genome-scale phylogeny and contrasting modes of genome evolution in the fungal phylum Ascomycota

Implications of some genetic control mechanisms in Neurospora.

Observations on the Tube Method of Measuring Growth Rate in Neurospora crassa

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