Growth of new bone guided by implants in a murine calvarial model

Freilich, Martin; Patel, C.; Wei, Mei; Shafer, David; Schleier, Peter; Hortschansky, Peter; Kompali, Raji; Kuhn, Liisa T.

doi:10.1016/j.bone.2008.05.021

Cited by 23 publications

(31 citation statements)

References 34 publications

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“…However, osteoblasts do not form bone directly on these materials, which limits their usefulness in the study of osseointegration. Lastly, titanium ring implants were studied in murine calvaria [60]. However, this study was focused on vertical bone growth around the ring implants, rather than integration.…”

Section: Discussionmentioning

confidence: 99%

Surface contaminants inhibit osseointegration in a novel murine model

Bonsignore

Colbrunn

Tatro

et al. 2011

Bone

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Surface contaminants, such as bacterial debris and manufacturing residues, may remain on orthopaedic implants after sterilization procedures and affect osseointegration. The goals of this study were to develop a murine model of osseointegration in order to determine whether removing surface contaminants enhances osseointegration. To develop the murine model, titanium alloy implants were implanted into a unicortical pilot hole in the mid-diaphysis of the femur and osseointegration was measured over a five week time course. Histology, backscatter scanning electron microscopy and x-ray energy dispersive spectroscopy showed areas of bone in intimate physical contact with the implant, confirming osseointegration. Histomorphometric quantification of bone-to-implant contact and peri-implant bone and biomechanical pullout quantification of ultimate force, stiffness and work to failure increased significantly over time, also demonstrating successful osseointegration. We also found that a rigorous cleaning procedure significantly enhances bone-to-implant contact and biomechanical pullout measures by two-fold compared with implants that were autoclaved, as recommended by the manufacturer. The most likely interpretation of these results is that surface contaminants inhibit osseointegration. The results of this study justify the need for the development of better detection and removal techniques for contaminants on orthopaedic implants and other medical devices.

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Section: Discussionmentioning

confidence: 99%

Surface contaminants inhibit osseointegration in a novel murine model

Bonsignore

Colbrunn

Tatro

et al. 2011

Bone

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“…)], the calvaria of mice [17; (Freilich et al. )] and the mandible of monkeys [17; (Hurzeler et al. )].…”

Section: Resultsmentioning

confidence: 99%

Systematic review of pre‐clinical models assessing implant integration in locally compromised sites and/or systemically compromised animals

Thoma

Martín

Mühlemann

et al. 2012

J Clinic Periodontology

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Objective: The aim was to systematically search the dental literature for pre-clinical models assessing implant integration in locally compromised sites (part 1) and systemically compromised animals (part 2), and to evaluate the quality of reporting of included publications. Methods: A Medline search was performed, complimented by additional hand searching. The quality of reporting of the included publications was evaluated using the 20 items of the ARRIVE (Animals in Research In Vivo Experiments) guidelines. Results: One-hundred and seventy-six (part 1; mean ARRIVE score = 15.6 ± 2.4) and 104 (part 2; 16.2 ± 1.9) studies met the inclusion criteria. The overall mean score for all included studies amounted to 15.8 ± 2.2. Housing (38.3%), allocation of animals (37.9%), numbers analysed (50%) and adverse events (51.4%) of the ARRIVE guidelines were the least reported. Statistically significant differences in mean ARRIVE scores were found depending on the publication date (p < 0.05), with the highest score of 16.7 ± 1.6 for studies published within the last 2 years. Conclusions: A large number of studies met the inclusion criteria. The ARRIVE scores revealed heterogeneity and missing information for selected items in more than 50% of the publications. The quality of reporting shifted towards betterreported pre-clinical trials within recent years.

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“…The selection of appropriate animal models in the test process keeps developing along with the development and modification of biomaterial implants in orthopedic and dentistry (Pearce et al 2007). Some of animal models that are commonly used in in vivo material test in orthopedic and dentistry implants are small-size animals (Mapara et al 2012), for example mice (Freilich et al 2008;Yang et al 2011), rats (Piccinini et al 2014) and rabbits (Mapara et al 2012). Whereas large animals (Gardel et al 2013;Pearce et al 2007), like sheep (Ulum et al 2014a), goat , dog (Baas 2008), and pig (Ruehe et al 2009) are also used in in vivo test.…”

Section: Appropriate Animal Modelsmentioning

confidence: 99%

Animal Study and Pre-clinical Trials of Biomaterials

Noviana

Estuningsih

Ulum

2016

Advanced Structured Materials

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An in vivo study is one of the most important steps in the process of translating biomaterials to clinical applications. It is mostly conducted to confirm in vitro results before going further to clinical trials. Appropriate use of animal models in the in vivo studies of biomaterials and medical devices is mandatory and should meet the approved regulations and ethics as defined by both local and international regulatory bodies. These studies involve the use of various approaches and protocols in order to know the body responses both local and systemic and to find out the short-and long-term responses of the body toward the implanted biomaterials or devices. This chapter describes complete procedures and practices of in vivo studies starting from selection of appropriate animal model, pre-implantation, surgical procedure and post-implantation, and monitoring of material-host responses. Some experiences of in vivo studies done by Indonesian researchers and the development of new implants are also presented.

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Growth of new bone guided by implants in a murine calvarial model

Cited by 23 publications

References 34 publications

Surface contaminants inhibit osseointegration in a novel murine model

Surface contaminants inhibit osseointegration in a novel murine model

Systematic review of pre‐clinical models assessing implant integration in locally compromised sites and/or systemically compromised animals

Animal Study and Pre-clinical Trials of Biomaterials

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